Tag Archives: presentation

Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients


Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients.
Here a film made of my ‘powerpoint’ presentation Feb 22, 2000 – on behalf of our Surgical Department – at the Opening Symposium of the Interdivisional GMP (‘Good Manufacturing Practice’) facility of the Leiden University Medical Centre – the LUMC in Leiden, Holland.

Background
I first started – with a background in biochemistry and philosophy – some 20 years earlier (from 1981) research at the Department of Surgery of the Leiden University Hospital (AZL) in the field of transplantation of the pancreas organ to cure diabetes.

Succesful Experiment Islet Transplantation
From 1986 my focus shifted to innovative procedures for the isolation out of the pancreas organ, of the ca 1 million islets of Langerhans – the insulin producing cell clumps with a size between ca 0.1 to 1 mm , that is 0.004 to 0,04 inch.
In 1989 we performed the first successful autologous transplantations in Holland (in collaboration with the Minneapolis Islet Lab with Jane Field and David Sutherland) – for the first time world-wide with pure islets using our novel procedure of using the organ preservation UW solution (University of Wisconsin / collaboration with dr Robert Carter , Du Pont, UK) during isolation in a pre-clinical large animal diabetes model – with long term transplant functioning with near-normal blood sugar regulation.

First series of human islet isolations , 1989-1990
Next in 1989-1990 we performed a first series of islet isolations from human donor pancreases using our novel techniques of isolation and purification in UW organ preservation solution – in association with a European Concerted Action for the Treatment of Diabetes – the Brussels headed (Daniel Pipeleers) ‘Multicenter program on the treatment of diabetes by islet cell transplantation’.

Exploring human islet work
Subsequently – after some years of exploring options for animal to human islet transplantation , using pig pancreas islets – so-called xenotransplantation – I was asked in 1997 to explore the requirements for operationalization of clinical islet transplantation in the LUMC. December 1997 our Surgical Department decided to go for it.

Establishing the human islet isolation laboratory in Leiden.
In 1998 after a month working in dr Camillo Ricordi’s Diabetes Research Institute in Miami, I started a large series of human islet isolation procedures in Leiden, and from september 1998 documenting the detailed GMP (‘Good Manufacturing Practice’) standard operating procedures, as well as ‘building’ our clinical islet isolation clean room with help from the Miami team in close cooperation with dr. Amon Wafelman (department of Clinical Pharmacy & Toxicology) managing the building of our brand new Interdivisional GMP-facility LUMC (IGFL) of six clean rooms and two chemical product laboratories – that was opened officially Feb 22, 2000 with the symposium, a film, and a tour in the GMP facility.
One year later the GMP islet facility was fully operational and summer 2001 we got a cautious green light (GMP license) for clinical transplantation of any high quality – approved – islet preparations.
Around the same time islet transplantation suddenly became very promising with ‘The Edmonton protocol’. However with the fast increase of logistic and financial demands, and reorganization between LUMC divisions it took till 2007 to finally start first transplants by an expanded islet team in the LUMC clinical islet center in Leiden.
Worldwide since 2000 several hundred people have received islet transplants that generally do not result in long term insulin independence , but do help sugar regulation in the transplant recipients.

Notes :
Links to some publications, news paper reports etc will follow later.

Updates:

Post updated with more details 20191215

Presentation ‘Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients’ by Michel van der Burg, Feb 22, 2000 – Opening Symposium of the Interdivisional GMP facility of the Leiden University Medical Centre, Leiden, Netherlands. Film : 20191213 Michel van der Burg | miracles.media

Push The Reset Button – Album Didier Jans


Singer Didier Jans and the author of his songs Béatrice Dethy together presented their latest album ‘Push The Reset Button’ (Remettre à zéro les compteurs) to Koenraad Tinel last Saturday , Feb. 9th 2019, in the Handelsbeurs theater in Gand , Belgium.

Koenraad Tinel
The extraordinary live performance of Koenraad Tinel last Saturday in the play As Long As He Does Not Know Himself was a great opportunity to watch Koenraad’s live work , and after the show to meet Koenraad and get the chance to personally present him their new album.

Liberated – tribute to the friendship between Simon Gronowski and Koenraad Tinel
The album includes the chanson ‘Liberated’ (LIBERES) they made in tribute to the extraordinary friendship between Simon Gronowski and Koenraad Tinel – inspired by the belgian documentary War Children (director Marianne Soetewey- VRT, 2012) and the book Liberated at Last (2013 ) that Simon and Koenraad together made on their friendship .

① memo 20190212 ~ Push The Reset Button – Album Didier Jans

Links :

Didier Jans
http://www.didierjans.com

Koenraad Tinel
http://www.koenraadtinel.be

Handelsbeurs theater in Gent / Gand , Belgium
https://www.handelsbeurs.be/en

Live performance – As Long As He Does Not Know Himself (Zolang Hij Niet Zichzelve Kent) https://www.lod.be/en/productions/zolang-hij-niet-zichzelve-kent

LIBERES chanson Liberated , Didier Jans
https://michelvanderburg.com/2018/11/20/liberes-chanson-didier-jans-2/

Finally liberated after 70 years
https://michelvanderburg.com/2013/03/13/finally-liberated-fr/

Liberated at Last – book presentation
https://michelvanderburg.com/2013/05/10/finally-liberatated-no-blame-no-victim-book-presentation-30-april-2013-in-brussels/mvdb20130430-165947_frames01/

Simon Gronowski and Koenraad Tinel present "Finally Free after 70 years" on Sept. 7, 2013 at the 13th Internationales Literaturfestival Berlin

Simon Gronowski and Koenraad Tinel present “Finally Free after 70 years” on Sept. 7, 2013 at the 13th Internationales Literaturfestival Berlin – with Vincent von Wroblewsky (interpreter) and Christine Eichel (host), Joachim Sartorius (Berliner Festspiele), Ulrich Schreiber (festival director), and Joke Schauvliege (Flemish Minister for Culture) in the Haus der Berliner Festspiele.
Photo report (BUM10022V01): Michel van der Burg – michelvanderburg.com

Finally liberated - no blame, no victim. Book presentation 30 April 2013 in the Filigranes library Brussels by Herman Van Goethem (Conservator Kazerne Dossin) and the authors Simon Gronowski and Koenraad Tinel.  Available in French as 'Enfin libérés' and in Dutch as 'Eindelijk bevrijd'. More info Photo report (video frames): Michel van der Burg

Finally liberated – no blame, no victim.
Book presentation 30 April 2013 in the Filigranes library Brussels by Herman Van Goethem (Conservator Kazerne Dossin) and the authors Simon Gronowski and Koenraad Tinel.
Available in French as ‘Enfin libérés‘ and in Dutch as ‘Eindelijk bevrijd‘. More info
Photo report (video frames): Michel van der Burg