1999 — Pioneering the LUMC Clinical Islet Transplant Project • 1MEMO_20260705

by Michel van der Burg , Sunday 5 July 2026 , Updated 10 July 2026

Abstract

In 1997, Onno Terpstra, Head of Surgery at Leiden University Medical Center (LUMC), asked Michel van der Burg to develop a plan for a clinical islet isolation and transplantation program. Following a working visit to the Diabetes Research Institute in Miami in spring 1998, where the Iodixanol-UWS purification method was tested on human islets, van der Burg began the islet isolation program at LUMC and the setting up of the Clinical Islet Laboratory (Klinisch Eilandlaboratorium, KEL) within a new GMP facility at LUMC, in collaboration with Amon Wafelman, executive head of the GMP facility. A business plan was submitted to the LUMC Board of Directors in November 1998 and approved on February 22, 1999, formally launching the project. The first series of human islet isolations, using manual pancreas digestion and Iodixanol-UWS purification, began in March 1999. The results were published in December 1999 and presented at the AIDSPIT Workshop in January 2000. From 2000 onward, the laboratory adopted the automated Ricordi method, using equipment supplied through BioRep Technologies in Miami.

Citation info : 1999 — Pioneering the LUMC Clinical Islet Transplant Project • 1MEMO_20260705 • Michel van der Burg • Miracles•Media • URL michelvanderburg.com/2026/07/05

1999 — No Loss of Human Islets in LUMC • 1MEMO_20260705_4_0 • Michel van der Burg • Miracles•Media • TakeNode 28097648-0b1d-4c1e-94cc-52e57738f04a • Presentation of the outcome of the first series of human islet isolation performed in 1999 in the Leiden University Medical Center (LUMC).

The LUMC clinical islet transplantation project began in 1997 on the initiative of the Department of Surgery at the LUMC, where I had been conducting animal-experimental research within the Transplantation Section since 1981 — first into the optimal method for pancreas transplantation, and subsequently into the transplantation of islets of Langerhans following isolation and purification of the islets from the pancreas (1-11).

Following successful animal-experimental islet transplantation, and a brief period of experimental human islet isolation in the early 1990s (8,9), Onno Terpstra, the head of our Surgery Department, asked me in the summer of 1997 to explore our options for starting clinical islet isolation and transplantation at our center — the Leiden University Medical Center (LUMC) — and to prepare a roadmap.

After first gaining experience in the spring of 1998 with the human islet transplant program in Miami — during which, in the course of isolating human islets of Langerhans, our new islet purification method also proved successful (11,12) — I was able to begin actually setting up the human islet project in Leiden in the summer of 1998.

Our innovative procedure for purifying islets in Iodixanol-UWS was further developed and published that summer of 1998 in collaboration with Nycomed (10).

The LUMC had recently begun renovating a wing of the building to establish a GMP facility with six so-called cleanrooms, intended to guarantee maximum protection and quality in the manufacture of medicinal products. After the summer of 1998, I was just in time to join those renovation plans, working together with Amon Wafelman — the executive head of the future GMP facility — to realize the necessary modifications in the GMP wing, with one of the six cleanrooms specially fitted out for use as our clinical islet laboratory. Although the cleanrooms had not originally been intended for permanent use, round-the-clock use of a cleanroom for the islet project could nonetheless become an option, with support from the Board of Directors.

By November 1998, I had incorporated a plan for setting up the Clinical Islet Laboratory (KEL) — and for the first 20 islet isolation procedures — into a business plan presented on behalf of our Department of Surgery to the LUMC Board of Directors (13; 1MEMO_20260705_1).

Business Plan CITX • 1MEMO_20260705_1_Front • Michel van der Burg • Miracles•Media • TakeNode 84c8fbb7-1e21-4e63-8940-3a79db30a0fa • Business plan Clinical Islet Transplantation Project (CITX) prepared November 3, 1998 for the Board of Directors of the LUMC . Front page image of the PDF file in Notes (13; 1MEMO_20260705_1) .

On February 22, 1999, the LUMC Executive Board approved the plan and authorized the necessary material resources, officially launching the islet transplantation project — as announced that month in the LUMC magazine ‘Cicero’ (14; 1MEMO_20260705_2).

‘The Transplantation of Islets of Langerhans: A Leiden First’, a pull quote from the feature story “Ernstiger ziek en wèl genezen” by Evert Pronk, in the LUMC news magazine Cicero, 5 February 1999 (ISSN 0920-2900) • 1MEMO_20260705_2

Quote (my translation):

“The Transplantation of Islets of Langerhans: A Leiden First

… The isolation of islets will take place at the LUMC. What remains is the completion of the GMP facility (Good Manufacturing Practice), the laboratory whose quality standards are so stringent that “products” can be manufactured that are suitable for administration to humans …”

The project was registered with the Eurotransplant organization for the allocation of donor organs — marginal donor organs, i.e. not suitable for clinical transplantation — to start up the islet isolation procedure, without transplantation being the aim at that stage.

In March 1999, we received the first donor organs via Eurotransplant for an initial experimental series of islet isolations. With the approval of the Board of Directors — since the GMP facility was still under construction — work could already begin in the Surgery Laboratory, gaining hands-on experience with human islet isolation, the donor procedure, islet culture, and all other facets of operating an “islet bank.”

Help for diabetic glands • April 1999 News Volkskrant • 1MEMO_20260705_3 • Miracles•Media • TakeNode ccbd39ce-9498-4bc9-aefb-96ae6f9e5791 • Help for diabetic glands (dutch: Hulp voor suikerzieke klieren) in Dutch national newspaper De Volkskrant , 10 April 1999 , by John Ekkelboom

In April 1999, the Dutch national newspaper ‘De Volkskrant’ announced the LUMC project in the article “Help for diabetic glands” (“Hulp voor suikerzieke klieren”) by John Ekkelboom (15; 1MEMO_20260705_3), with contributions from Jan Ringers (Head of the Transplantation Section of our department) and myself.
Excerpts (translated from Dutch):

“Help for diabetic glands

Some patients with juvenile diabetes may benefit from a transplantation of islets of Langerhans. Cells from these islets replenish the insulin deficiency. The Leiden University Medical Center (LUMC) will soon treat several patients with this new method.

… Dr. Jan Ringers, a transplant surgeon at the LUMC, says that the chance of success has increased to such an extent that his hospital now also considers the procedure justifiable …

… Dr. Michel van der Burg attributes the fact that this scenario did not go as well abroad in the early years to, among other things, less suitable enzymes and the premature insertion of the transplant. As a scientific researcher at the LUMC, he focuses on the isolation of the islets …”

— John Ekkelboom, April 10, 1999, Volkskrant

First series of human islet isolations in Leiden

In March 1999, the first series of human islet isolations began in the LUMC Surgery Laboratory, applying the manual method of donor pancreas digestion, with purification of the islets in the new Iodixanol-UWS gradients.

Human islets of Langerhans prepared by purification in the Iodixanol-UWS solution by Michel van der Burg at the start of the LUMC islet transplant project, March 1999, Leiden University Medical Center (LUMC), Leiden, Holland • 1MEMO_20260623_1 • Michel van der Burg • Miracles•Media • TakeNode 30b1f224-f963-480e-838e-42b4f8dfa559

This Iodixanol-UWS purification method had been developed in our animal-experimental research on the isolation and purification of porcine islets for successful transplantations, first in diabetic mice, then in rats and monkeys (7,16).

The Iodixanol-UWS purification method had also proved promising the previous year in my pilot research during human islet isolations at the Diabetes Research Institute in Miami, where the purified human islets were successfully transplanted into nude mice (11).

The results of our first series of human islet isolations at the LUMC in 1999 were published in December 1999 and subsequently presented at the 19th AIDSPIT Workshop of the European Association for the Study of Diabetes (EASD) in Igls, Austria, in January 2000 (17,18,19,20; 1MEMO_20260705_4).

No Loss of Human Islets • AIDSPIT 2000 • 1MEMO_20260705_4 • Michel van der Burg • Miracles•Media • Outcome 1st series in 1999 of human islet isolation in LUMC presented at the 19th AIDSPIT Workshop of the European Association for the Study of Diabetes (EASD) in Igls, Austria, January 23–25, 2000 . Details , plus handout and abstract for download in Notes (17,18,19,20).

State of the Art — Ricordi Method & BioRep Technologies

Work then began on the second series of isolations, this time using the state-of-the-art “Ricordi Automated Method” for pancreas digestion (21), as further developed by Camillo Ricordi, first in David Scharp’s team in Paul Lacy’s laboratory in St. Louis (USA) and eventually in Miami together with Ramon Poo of BioRep Technologies, Inc.

In setting up the state-of-the-art procedure for islet isolation from the pancreas using the Ricordi Automated Method at the LUMC, I worked at the end of 1998 together with Gerard Verschagen, head of the precision mechanics workshop of the Laboratory of Physiology of the LUMC (22), on developing a compressed-air-driven shaking device for the Ricordi chamber, for which modifications to the GMP complex were also planned.

Earlier in 1998, during my working visit to the Diabetes Research Institute in Miami, I had met Mr. Ramon Poo, who — alongside his day job as a designer and manufacturer of plastic bottles in Miami (Altira, Inc.) — also contributed on a voluntary basis to Ricordi’s islet project in Miami. Through his firm founded specifically for this purpose, BioRep Technologies, Inc., Poo designed and manufactured the special equipment for the Ricordi isolation method, including the digestion chamber and a shaking device for the Ricordi chamber (23,24). During that working visit, Mr. Poo also gave me a stainless-steel Ricordi chamber and other components for the “Ricordi setup” to start up the procedure in our laboratory in Leiden, Holland, in the LUMC.

During discussions at the end of 1998, by phone and fax, with Mr. Poo about our design in Leiden of the shaking device based on the BioRep model, it emerged that BioRep could supply us with a new model of shaking device — the Wrist Shaker, a model not yet patented — with the permission of Camillo Ricordi’s team, on condition of “no disclosure.”

From 1999 onward, the Wrist Shaker (Model No. B-3-G) and other BioRep equipment were imported, after which, from 2000 onward, the Ricordi Automated Method for pancreas digestion was applied for islet isolation at the LUMC…to be continued…

Notes

1) Pancreatic islet transplantation Thesis Repository Leiden University • 20221125 • Michel van der Burg • Miracles•Media • . Persistent URL https://hdl.handle.net/1887/3486604

2) Leiden Islet Laboratory History • 20240803 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/08/03/leiden-islet-laboratory-history-20240803/

3) Pancreas & Islet Transplantation Program • 20240912 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/09/12/pancreas-islet-transplantation-program-20240912/

4) Pancreatic Islet Isolation With UW Solution • A New Concept • Michel van der Burg • Miracles.Media • @1MEMO 20241224 • URL michelvanderburg.com/2024/12/24/

5) Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/01/14/

6) Islet Transplantation Breakthrough in Leiden University Hospital • 20240830 • Michel van der Burg • michelvanderburg•com URL https://michelvanderburg.com/2024/08/30/islet-transplantation-breakthrough-in-leiden-university-hospital-20240830/

7) Successful Islet Transplantation After Purification in Iodixanol-UWS • IPITA 1999 Sydney • @1MEMO 20260326 • Michel van der Burg • Miracles.Media • URL michelvanderburg.com/2026/03/26/

8) 1989 — First series of human islet isolations in Leiden. In 1989-1990 we performed a first series of islet isolations from human donor pancreases using our novel techniques of isolation and purification in UW organ preservation solution – in association with a European Concerted Action for the Treatment of Diabetes – the Brussels headed (Daniel Pipeleers) ‘Multicenter program on the treatment of diabetes by islet cell transplantation’.

9) Pittsburgh 1992 Cell Transplant Society • 20240812 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/08/12/pittsburgh-1992-cell-transplant-society-20240812/

10) Islet purification from bench to bedside • @1MEMO_20260623 • Michel van der Burg • Miracles.Media • URL michelvanderburg.com/2026/06/23/

11) Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL michelvanderburg.com/2024/12/02/

12) Human Islet Isolation • DRI Miami 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250204 • URL michelvanderburg.com/2025/02/04/

13) Business Plan CITX • 1MEMO_20260705_1 • Michel van der Burg • Miracles•Media • TakeNode 62317860-28ff-46a6-9e2b-20910f7f4aa6 • Business plan Clinical Islet Transplantation Project (CITX) prepared November 3, 1998 for the Board of Directors of the LUMC (English translation by author). PDF for viewing and download below 1MEMO_20260705_1.

1MEMO_20260705_1 Download

14) A Leiden First • 1MEMO_20260705_2 • Miracles•Media • TakeNode d3d3effe-ee4c-479c-8051-c78a90746eb1 • “The Transplantation of Islets of Langerhans : A Leiden First “. Pull quote from the feature story ‘Ernstiger ziek en wèl genezen’ by Evert Pronk, in the LUMC news magazine Cicero, 5 February 1999 (ISSN 0920-2900) .

15) Help for diabetic glands • April 1999 News Volkskrant • 1MEMO_20260705_3 • Miracles•Media • TakeNode ccbd39ce-9498-4bc9-aefb-96ae6f9e5791 • Help for diabetic glands (dutch: Hulp voor suikerzieke klieren) in Dutch national newspaper De Volkskrant , 10 April 1999 , by John Ekkelboom . Text (only) online in Volkskrant archive URL https://www.volkskrant.nl/wetenschap/hulp-voor-suikerzieke-klieren~b7046616/

16) Primate Islet Isolation and Purification in Iodixanol-UWS • @1MEMO_20260425 • Michel van der Burg • Miracles.Media • URL michelvanderburg.com/2026/04/25/

17) Van der Burg MPM, Ringers J, Baranski A, Bouwman E, Terpstra OT. No loss of human (mantle-) islets by OptiPrep-UWS purification following isolation in UWS. 19th AIDSPIT Workshop of the European Association for the Study of Diabetes (EASD) in Igls (Austria) Jan 23–25, 2000 . Abstract (Page 230) in : Acta Diabetol 36, 205–231 (1999). https://doi.org/10.1007/s005920050168 . PMID: 10664329

18) No Loss of Human Islets • AIDSPIT 2000 • 1MEMO_20260705_4 • Michel van der Burg • Miracles•Media • Video of slideshow TakeNode bb4091a1-374f-4a04-86a1-67e371074109 • URL https://youtu.be/txSFax_nR2I

19) Ibid. Handout of slideshow : 1MEMO_20260705_4_PDF • Michel van der Burg • Miracles•Media • TakeNode 35766a9b-d1b1-47af-9371-4edca9c3ad0d • Download PDF below ( 1MEMO_20260705_4_PDF )

1MEMO_20260705_4_PDF Download

20) Van der Burg MPM, Ringers J, Baranski A, Bouwman E, Terpstra OT. No loss of human (mantle-) islets by OptiPrep-UWS purification following isolation in UWS (Abstract). Acta Diabetol 36, 230 (1999). URL https://doi.org/10.1007/s005920050168 . PMID: 10664329

Ibid. Abstract copy : 1MEMO_20260705_4_PMID_10664329 • Michel van der Burg • Miracles•Media • TakeNode fa10d5e9-3df9-46e0-b8cf-7f9f9f853526 • Download PDF below ( 1MEMO_20260705_4_PMID_10664329 )

1MEMO_20260705_4_PMID_10664329 Download

21) Piemonti L, Pileggi A. 25 YEARS OF THE RICORDI AUTOMATED METHOD FOR ISLET ISOLATION. CellR4 Repair Replace Regen Reprogram. 2013;1(1):e128. PMID: 30505878; PMCID: PMC6267808. URL https://pmc.ncbi.nlm.nih.gov/articles/PMC6267808/

22) Leiden Islet Isolation Chamber • Michel van der Burg • Miracles.Media • @1MEMO 20250116 • URL michelvanderburg.com/2025/01/16/

23) How a Family Business Has Buoyed the Diabetes Research Institute (DRI) URL https://www.mwhoskinsmedia.com/2019/12/how-family-business-has-buoyed-diabetes.html

24) Who we are. Biorep. URL https://biorep.com/who-we-are/

#islets #islet #Langerhans #isolation #purification #isletbank #first #launch #Cicero #Volkskrant #newspaper #news #GMP #cleanroom #facility #Surgery #KEL #CITX #LUMC #Holland #Netherlands #Leiden #hospital #Eurotransplant #human #donor #surgery #clinical #laboratory #optiprep #iodixanol #ViaSpan #density #gradient #UWS #pancreas #UW #university #Wisconsin #transplantation #diabetes #man #history #research #history #memoir #1memo #michelvanderburg #BioRep #CamilloRicordi #DRI #Miami #Nycomed #Visipaque

Citation info : 1999 — Pioneering the LUMC Clinical Islet Transplant Project • 1MEMO_20260705 • Michel van der Burg • Miracles•Media • URL michelvanderburg.com/2026/07/05

Genocide • 20260519

Settela Steinbach — The Girl with the Headscarf • 1MEMO_20260519_1 • Settela•Com • Frame from camera original film reel of the Westerborkfilm (1).

On May 19, 1944, at the Westerbork transit camp, a glimpse of Sinti girl Settela Steinbach wearing a headscarf appears between the sliding doors of a cattle car awaiting deportation to Auschwitz (1,2,3).
In May 1945, her father, Moeselman Steinbach, wrote to “Repatriation” in the Netherlands: “…I very politely request you to inform me whether my wife and 10 children have arrived, or only children (Gypsy children) from the Auschwitz concentration camp in Poland.” (4).

The 9-year-old dutch Sinti-girl Anna Maria ‘Settela’ Steinbach peeks outside , at the last moment just before the sliding door is closed , standing inside a cattle car with 74 people on May 19 , 1944 in the Westerbork transit camp in Holland , when this deportation train leaves for Auschwitz-Birkenau – where Settela is murdered a few months later in one of the gas chambers (5).

While Settela peeks outside , her mother cries behind her in that cattle car : “Get out of there, or soon your head gets in between!”

She is wearing a headscarf made from a torn sheet because the Nazis shaved her head upon arrival at Westerbork transit camp on May 16, 1944, following the “Gypsy raid” carried out that same day at the Zwaaikom caravan site in Eindhoven, the Netherlands (6).

Settela Film • 20220630

Settela was filmed only a few seconds by the Jewish prisoner filmmaker Rudolf Breslauer as part of a documentary film being made in 1944 on the Westerbork camp .
Those seconds , also in slow-motion are shown in the 2022 Settela Film • 20220630 (7)

The toddler Settela in the arms of her older sister Elisabeth Steinbach at the Heksenberg Sinti caravan site in 1935 • Photo Jan de Jong • 1MEMO_20260518_4

Anna Maria (‘Settela’) Steinbach was born 23 December 1934 in Buchten, Netherlands, and photographed at age ~1 , in the arms of her older sister Elisabeth Steinbach, with others of the Steinbach family, and other families, at the nearby Sinti caravan site ‘Heksenberg’, October 1935, by photographer Jan de Jong (8) • 1MEMO_20260518_4

Settela was deported together with her brothers and sisters (Willy “Celestinus”, Willem, Elisabeth, Johanna, Philibert, Florentina, Willem, Anna), and mother Toetela (Emilia) Steinbach (born 23 March 1902 in Antwerp, Belgium), with other Steinbach and other nomad families – all together ca 245 Sinti and Roma and ca 450 Jews – on May 19th 1944 from the dutch Camp Westerbork to the Auschwitz and Bergen-Belsen camps (5,6).

Toetela’s eldest child Moekela (Magdalena; born 14 Sep 1922) had gone to Belgium and had been deported earlier – 15 Jan 1944 – with her 6 months old baby Jeanette – Toetela’s granddaughter – on the Z-Transport from transit camp Kazerne Dossin in Mechelen to Auschwitz, were they were murdered on arrival.

Settela’s father Heinrich (‘Moeselman’) Steinbach (born Nov 11, 1901 in Gründorf in Germany) died alone of grief June 6, 1946 in Maastricht in the Netherlands – his wife and 10 children had not survived the camps.

To : “Repatriation” in Maastricht (Netherlands) — “Dear Sirs, I very politely request you to inform me whether my wife and 10 children have arrived, or only children (Gypsy children) from the Auschwitz concentration camp in Poland.
From May 15, 1944, my children and wife were taken there; no Jews. And Weiss had to come along too. — Heinrich Steinbach. Caravan site Eindhoven , North Brabant” • 1MEMO_20260519_2 • Settela•Com

One year earlier , May 22, 1945, two weeks after the liberation of Holland, Heinrich Steinbach — living at the caravan site in Eindhoven (North Brabant, Netherlands) — inquires about the fate of his wife and ten children on a postcard written to the “Repatriation” in Maastricht (Netherlands). The text on the postcard reads — translated from dutch (4) :

“Dear Sirs, I very politely request you to inform me whether my wife and 10 children have arrived, or only children (Gypsy children) from the Auschwitz concentration camp in Poland.

From May 15, 1944, my children and wife were taken there; no Jews. And Weiss had to come along too.

Heinrich Steinbach. Caravan site Eindhoven N.B.”

Notes

1 – Settela Steinbach in Westerbork Film 🎦 2021 | 20220302 | Michel van der Burg | Settela•Com | ISSN 2949-9313 (accessed 2026 May 18) URL: https://www.youtube.com/watch?v=ZiLNDziwEtc&t=1012s

4 – May 1945 Postcard Heinrich Steinbach • 1MEMO_20260519_2 • Settela•Com | ISSN 2949-9313 | Source : Collectie HCL, archief Militair Gezag, Maastricht.
‘Moeselman’ Heinrich Steinbach — living at the caravan site in Eindhoven (North Brabant, Netherlands) — inquires about the fate of his wife and ten children on a postcard written to the “Repatriation” in Maastricht (Netherlands). The dutch text on the postcard reads :
“Geachte Heeren, Ik verzoek u zeer beleeft om mijn te willen berichten op mijn vrouw en 10 kinders aan gekomen zein of alleen kinders (zigeunerkinders) uit contrasie kamp uaschwietsch Polen.
Van 15 mei 1944 zein mijn kindeers en vrouw naar toe gebracht, geen joden. En ook Weiss moet ook mee komen. Heinrich Steinbach. Woonwagenkamp Eindhoven N.B.”

6 – ‘Zigeunerrazzia’ 16 mei 1944. Peter Jorna. Stichting 18 September (Eindhoven) URL https://stichting18september.nl/zigeunertransport/

8- Sinti Caravan Site • 20260518 | Michel van der Burg | Settela•Com | ISSN 2949-9313 | URL https://settela.com/2026/05/18

TAGS #Settela #girl #headscarf #Steinbach #Westerborkfilm #Westerbork #Breslauer #Roma #Sinti #gypsy #zigeuner #nomad #caravan #razzia #raid #Eindhoven #postcard #Holocaust #Porajmos #genocide #children #camp #deportation #Jew #Netherlands #railcar #train #station #transitcamp #UNESCO #film #letter #Heksenberg #Zwaaikom #Auschwitz #SettelaCom #MiraclesMedia #michelvanderburg

Liberation • 1MEMO_20260508

Liberation • 1MEMO_20260508 • Michel van der Burg • Miracles•Media • Cheering crowds greet British troops entering Brussels, 4 September 1944 . TakeNode 43cdc95e-4db6-44db-bd5b-fec92f198f86

Celebrating crowds, while the Allied troops drive the Germans out of the city, Sunday 3 September 1944 in Brussels, Belgium .

Source Image : BUM20200418_38 • Miracles.Media (BU483 , WikiMedia Commons)

Source Video : Brussels Liberated • @1MEMO_20200415 • Michel van der Burg • Miracles.Media • TakeNode 00e3f7fb-2455-4239-b1da-120f31e85fc3 • From : The Year 1944 United Newsreel ( 208 UN 1034 USHMM RG-60.3053 | Film ID: 2396 )
#liberation #celebration #occupation #street #crowd #dance #Brussels #Belgium #war #ww2 #MiraclesMedia #allies

Yodeling • Tyrolean Folksy Hootenanny 2026 #1 • Via Romea #53 • @1MEMO_20260327

Yodeling • Tyrolean Folksy Hootenanny 2026 #1 • Via Romea #53 • @1MEMO_20260327 • Michel van der Burg • Miracles•Media • TakeNode 2e07f99b-1bbc-48e9-aac6-02baec4a4339 •
Simple yodel song, celebrating spring at the Hoangascht – Hootenanny – along the Jakobsweg route — also the Via Romea here — in Gasthof Turmbichl, Vill, Tyrol, Austria, March 2026

Casual get-together of locals singing and playing folk & schlager music. for more of this Tyrolean folksy music , check it out at my channel, searching for ‘Hoangascht’ • https://www.youtube.com/@michelvanderburg/search?query=Hoangascht

Compilations
Viller Musikanten Hoangascht • @1MEMO 20180321 • https://youtu.be/2T8kc5vSGuM Recorded March 2016.

Inge’s Tyrolean Folk Music • @1MEMO 20250323 • https://youtu.be/6kUpDvVesKU

More Tyrol musical film work in the YouTube Playlist ‘Tyrol Music’ – https://youtube.com/playlist?list=PL_SggYHVHwj3eL6D8AzUm3o84OrU1JX_7

#music #folk #hoangascht #hootenanny #Vill #Innsbruck #Via #Romea #Tirolensis #pilgrim #route #path #Tyrol #Jakobsweg #Camino #Santiago #Alps #musicvideo #art

Successful Islet Transplantation After Purification in Iodixanol-UWS • IPITA 1999 Sydney • @1MEMO 20260326

Pig islets after isolation in Iodixanol-UWS • 7th IPITA World Congress 1999 • 1MEMO_20260326_1 • Michel van der Burg • Miracles•Media • TakeNode 467e773c-fc4b-416e-99d5-c88df76ddff4 • Presentation by Michel van der Burg (Notes #5).

Shortly after our introduction at the previous IPITA World Congress, September 1997 in Milan (Italy) of the novel Iodixanol -UWS solution for isolation of juvenile pig islets (1), we reported successful xeno-transplantation in diabetic Swiss nude (immunodeficient) mice of adult pig islets isolated with this Iodixanol -UWS method, first Tuesday, Jan 27, 1998 at the 17th Workshop of the AIDSPIT Study Group in Igls, Austria (2a), and next April 1998 at the 10th Congress of the Dutch Transplantation Society (2b). We had started these first pig islet xenotransplants in mice also that same month of September 1997 — highlighted in the image below ( 1MEMO_20260326_12 )

First pig islet xeno transplants in mice • 1MEMO_20260326_12 • Michel van der Burg • Miracles•Media • TakeNode d4cc1f11-c107-4011-8dbe-9649da76e3ff • September 1997, we performed our first two pig islet xenotransplants into diabetic Swiss nude mice. The pig islets were isolated with Iodixanol-UWS from an adult pig pancreas (top panel: sample of the islets stained red with dithizone, fresh after isolation and purification). These pure islets were kept 1 week in a culture solution, before transplantation of the islets by injecting the islets under the kidney capsule in 2 diabetic Swiss nude mice. Both mice had normal blood glucose from the day one after transplantation, and upon removal of the kidneys for examination at 1 month after transplantation , we observed excellent islets under the kidney capsule , as shown in the bottom panel image of a kidney section stained with H&E (note : the inserted portrait of the Swiss nude mouse, actually is our #7 pig islet xenograft, alive and kicking, Dec 1997).

Starting with the September 1997 IPITA meeting in Milan, I was sharing in detail the materials and methods of our purification procedure, face to face and in post-congress correspondence, with my colleagues of the other islets centers, thus facilitating fast implementation by Bernhard Hering and Shinichi Matsumoto of the Iodixanol-UWS purification method in the Minneapolis islet center, contributing to their successful single-donor pig islet allo-transplants, as reported by Shinichi Matsumoto May 1998 at the 24th Annual Scientific Meeting of the American Society of Transplant Surgeons, in Chicago (3).

That month, May 1998, I also finished the first series of human islet purifications with the novel Iodixanol-UWS density gradient, while working on human islet isolations in the Miami team at the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami , in Miami, Florida, USA . The successful outcome of the human islet purification work done in Miami was reported from January 1999 in a talk and poster presentations , including a poster presentation at the 7th World Congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 1999 (4).

Viability of Fresh vs Cultured Pig Islets for Transplants • 1MEMO_20260326_2

Slideshow presentation by Josephine Rijkelijkhuizen • 7th IPITA World Congress 1999 • Viability of Fresh vs Cultured Pig Islets for Transplants • 1MEMO_20260326_2 • Michel van der Burg • Miracles•Media • TakeNode 7164b724-65bf-4d3f-8a40-89b188957d1b • Notes #6

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Handout • PDF (Viability of Fresh vs Cultured Pig Islets for Transplants)
7th IPITA World Congress 1999 • 1MEMO_20260326_3 • Michel van der Burg • Miracles•Media • TakeNode 995e90b3-d797-435c-a510-00a0e35bc424 • Handout presentation by Josephine Rijkelijkhuizen (Notes #6).

In our oral presentations at the 7th World Congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 1999, we demonstrated both the complete recovery of pure intact pig islets during isolation in UWS and purification in the novel solution of Iodixanol (Optiprep) in UWS, and the superior viability of these islets following short-term storage in culture solution (‘culture’) , allowing successful xeno transplantation of the pig islets in mice (5,6), rats (7), and monkeys (8) .

The 7th IPITA congress abstracts were published online only at the official website of the IPITA, in Oxford at the time (www.jr2.ox.ac.uk/ipita), and cannot be retrieved now (9). However, I kept an archive with photocopies of our abstracts published in print in the Abstract Book of the 7th IPITA , posted below.

One of our papers was included among the top 10 papers selected for submission of a full paper in a special issue of the journal Cell Transplantation — see below.

Josephine Rijkelijkhuizen at the 7th IPITA World Congress 1999 • 1MEMO_20260326_4 • Photo by Michel van der Burg • Miracles•Media • TakeNode 0c2ef6fb-bdff-4895-9dde-eb242f957cb9

Top 10 Paper

Our study — collaborative research of our Surgery Department (LUMC), and workers of the Biomedical Primate Research Centre in Rijswijk (Netherlands) — presented by our student researcher Josephine Rijkelijkhuizen, showing that cultured pig islets, isolated and purified in Iodixanol-UWS, can be transplanted without hyperacute rejection in cynomolgous monkeys , was selected in the top 10 outstanding studies at the memorable Sidney World Congress of the International Pancreas & Islet Transplant Association (9) , and the full paper included in a special issue of the journal Cell Transplantation (10).

Our studies , together, demonstrated the importance of both donor organ selection (choosing adult organs), selection of non-toxic, endotoxin-free media, such as iodixanol and UWS, as the isolation solutions, and selection of islets that survive storage in culture, for a successful outcome after transplantation in this difficult model of xeno-transplantation, grafting porcine islets in other species.

Notes

1) Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250128 • URL michelvanderburg.com/2025/01/28/

2a) Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250131 • URL michelvanderburg.com/2025/01/31/

2b) Fresh vs Cultured Pig Islets for Xenografts • NTV Bootcongres 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250201 • URL michelvanderburg.com/2025/02/01/

3) Matsumoto S, Zhang H J, Gilmore T, van der Burg MPM, Sutherland DER, Hering BJ. Large scale isopycnic islet purification utilizing non-toxic, endotoxin-free media facilitate immediate single-donor pig islet allograft function (Abstract). Transplantation 1998; 66: S30 • 1MEMO_20260326_10 • Michel van der Burg • Miracles.Media • TakeNode a7e8ec21-1757-45f4-90c6-1a5f457e0d9f , View/download PDF below.

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Presentation at the 24th Annual Scientific Meeting of the American Society of Transplant Surgeons, Chicago (USA) May 1998. 24th ASTS Program book, Abstract #A-120 • 1MEMO_20260326_5 • Michel van der Burg • Miracles.Media • TakeNode 9efdd5cb-f6da-4dec-b9d9-63cf0b254b30

4) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. New powerful tool for human islet purification: OptiPrep-UWS? 7th World Congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Abstract in Programme and Abstract Book, page 169, P298 . In : New Powerful Tool For Human Islet Purification • OptiPrep-UWS ? • IPITA 1999 Sydney • @1MEMO 20260325 • Michel van der Burg • Miracles.Media • URL michelvanderburg.com/2026/03/25/

5) Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Programme and Abstract Book, page 164, #281 . 1MEMO_20241203_3 • In : Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • 1MEMO 20241203 • Michel van der Burg • Miracles.Media • TakeNode 64ace253-d25b-4b6d-af5b-08060712c471 • URL michelvanderburg.com/2024/12/03/

6) Rijkelijkhuizen JKRA, Bouwman E, Van der Burg MPM. Pre-transplant culture markedly improves pig islet graft survival in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Programme and Abstract Book, page 117, #162 . 1MEMO_20260326_6 • Michel van der Burg • Miracles.Media • TakeNode 5ce0c54f-a6a1-4cc9-8f68-9a1cd723badf

7) Rijkelijkhuizen JKRA, Van der Burg MPM, Bouwman E. Longterm survival of porcine islets in the rat. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Programme and Abstract Book, page 112, #152 . 1MEMO_20260326_7 • Michel van der Burg • Miracles.Media • TakeNode bc9a48ee-23ff-48f1-ab2c-5bcef071ebaa

8) Rijkelijkhuizen JKRA, Bouwman E, Van der Burg MPM, Ringers J, Ossevoort MA, Kuhn EM, Frost P, Jonker M. Successful suppression of the early rejection of pig islets in monkeys. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Programme and Abstract Book, page 144, #231 . 1MEMO_20260326_8 • Michel van der Burg • Miracles.Media • TakeNode 29bb03b4-8b02-4aa9-9b33-715ca006c28e

9) Ricordi C. Prime Time for Pancreas and Islet Transplantation at the Turn of the Millennium. Cell Transplant. 2000 Nov;9(6):853-855. doi: 10.1177/096368970000900611. PMID: 28886292. URL https://journals.sagepub.com/doi/epdf/10.1177/096368970000900611 (Free Full Paper)

10) Rijkelijkhuizen JK, Bouwman E, van der Burg MP, Ringers J, Ossevoort MA, Kuhn EM, Frost P, Jonker M. Successful suppression of the early rejection of pig islets in monkeys. Cell Transplant. 2000 Nov-Dec;9(6):909-12. doi: 10.1177/096368970000900618. PMID: 11202577. URL https://journals.sagepub.com/doi/epdf/10.1177/096368970000900618 (Free Full Paper) • Collaborative research From Biomedical Primate Research Centre (M.O., E.M.K.,
J.W., K.H., M.J.), Rijswijk, and Leiden University Medical Centre (J.K.R.A.R., J.R., M.vdB., E.B.), Leiden, The Netherlands .

Sydney Convention Center • 1MEMO_20260326_9 • Michel van der Burg • Miracles.Media • TakeNode 1ce6bbf2-adf1-4ce3-8c69-cd2d1339be81 • Sydney Convention Center – Darling Harbour (demolished in 2013)

Cultured pig islets for xenotransplantation • 1MEMO_20260326_11 • Michel van der Burg • Miracles•Media • TakeNode bd15f087-29cd-4e99-a555-9fbfbd1729bc • Reflection contrast microscopy (ultrathin section) of 1-week cultured pig islets . During storage in culture remodeling of the isolated islets occurs, showing rounded islets , with some central necrosis after prolonged culture. Transplantation of 1250 islets (IEQs) from this same 1-week cultured pig islet preparation cured all (n=3) diabetic nude mice indefinitely (over 150 days). In contrast transplants of freshly isolated islets (not cultured) using the same dose of 1250 islets (IEQs) from this same islet preparation, did not cure diabetic nude mice (n=2). Adult pig pancreas procurement by Michel van der Burg from the nearby Westgeest slaughterhouse (in Zoetermeer) facility for large sows processing by Ballering Export (Son, Netherlands; a company of second cousins, of mine).
. Islet isolation (using Iodixanol-UWS) and transplants team work by Josephine Rijkelijkhuizen and Michel van der Burg, Leiden Islet Laboratory (LUMC), May 1998. Reflection contrast microscopy by Frans A. Prins and Michel van der Burg. Not previously published data and photo by Michel van der Burg.

#IPITA #Sydney #surgery #DRI #Miami #clinical #laboratory #purification #optiprep #iodixanol #density #gradient #UWS #pancreas #human #pig #rat #mouse #monkey #xeno #islets #islet #Langerhans #isolation #UW #university #Wisconsin #transplantation #diabetes #man #history #Holland #Netherlands #Leiden #hospital #LUMC #1memo #michelvanderburg #Rijkelijkhuizen #Matsumoto #Hering #Minneapolis #Ricordi

Citation info : Successful Islet Transplantation After Purification in Iodixanol-UWS • IPITA 1999 Sydney • @1MEMO 20260326 • Michel van der Burg • Miracles.Media • URL michelvanderburg.com/2026/03/26/

24th AIDPIT Workshop Igls • @1MEMO 20260125

Short impression of the 24th Workshop of the AIDPIT Study Group (Artificial Insulin Delivery, Pancreas and Islet Transplantation), in Igls (Innsbruck) Austria, 23-25 January 2005.

Video from January 25th featuring :

(i) the Igls Congress park ;

(ii) the morning opening lecture by Mathias Brendel, with chairs Bart Keymeulen (Brussels, Belgium) and Mathias Brendel (Giessen, Germany), and, in the lecture hall : president Annika Tibell (Sweden) and honorary member Reinhard Bretzel (Germany) ;

(iii) a lunch time meeting with José Oberholzer (Chicago, USA) … not familar yet 😉 with that new little Sony T1 camera with a periscopic lens inside .. ;

(iv) one of the afternoon discussion groups (Clinical Islet Transplantation) ;

(v) clips from the plenary oral abstract session with chairs Karin Ulrichs (Germany) and Richard Smith (Bristol , UK) and lectures by Norma Sue Kenyon (Miami , USA) and Piero Marchetti (Pisa, Italy) with discussion by Daniel Brandhorst.

Notes

AIDPIT was known first as AIDSPIT, short for : Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation.
The AIDSPIT study group of the European Association for the Study of Diabetes (EASD) was established in the early 1980s.
In 2000 the acronym AIDSPIT was replaced by ‘AIDPIT’, in order to avoid confusion with the AIDS disease.

The annual meetings are generally held in the little town of Igls, close to Innsbruck in Austria — the first years, early in February, and next, late in January. Once, in the 1990s (following a discussion ‘…why always in Igls?’, the meeting was held in Amsterdam.
From 2011, the annual workshops of AIDPIT continued as a joined meeting with the EPITA — the European Pancreas and Islet Transplant Association — part of the European Society for Organ Transplantation (ESOT).
From 2024 — following the 12th EPITA Symposium & 41st AIDPIT Workshop in 2023 — this symposium is referred to as the EPITA Symposium.

The original film (2016) was uploaded to Vimeo (size 640×480), and posted Jan 25, 2016 (20160125) at blogging site 1-memo.com , URL https://1-memo.com/2016/01/25/24th-aidpit-workshop-igls/

A second, remastered, video edition (2022) was produced by upscaling the original video file (converted to size 1920 × 1440) and adding a caption, and uploaded to YouTube Jan 25, 2022.

This second edition is published now – Jan 25th 2026 – as part of the Science series of posts at my site michelvanderburg.com, started in 2022 to make available online a portfolio of the science section of my CV, a project I hope to finish soon… in a few months.

Portfolio – Science : https://michelvanderburg.com/category/science/

#AIDPIT #EPITAsymposium #Igls #Innsbruck #Tyrol #history #ESOT_EPITA ##AIDSPIT #Austria #islet #Langerhans #isolation #transplantation #pancreas #diabetes #insulin #michelvanderburg #symposium #EASD #ESOT #workshop #EPITA #science

Citation info : 24th AIDPIT Workshop Igls • Michel van der Burg • Miracles•Media • @1MEMO 20260125 • TakeNode 3baced38-ee79-4127-932d-0136293529e7 • URL https://michelvanderburg.com/2026/01/25

Glycemic control mechanisms after canine islet autografting • IPITA 1995• @1MEMO 20251204

Glycemic control mechanisms after canine islet autografting • IPITA 1995 • Michel van der Burg • Miracles.Media • @1MEMO 20251204_1 • Talk, Tuesday June 20, 1995 at 10:30, 5th IPITA, Eden Roc Resort Hotel, Miami Beach, FL (USA) • Digitally remastered by author, publisher, Michel van der Burg • TakeNode 5227c200-e029-4406-b344-15350fe3b93d • #GLP1 #GIP #diabetes #islets #transplant

Pioneer work on GLP-1 and GIP incretin hormones

Slideshow (digitally remastered) of my talk Tuesday June 20, 1995 at 10:30, at the 5th International Congress on Pancreas and Islet Transplantation (IPITA), in Miami Beach, FL (USA). My first talk at a world congress presenting the outcome of all our detailed metabolic studies in our preclinical model of canine islet autotransplantation , including our pioneer work on the incretin effects of the gut hormones GLP-1 (glucagon-like peptide-1) and GIP (Glucose-dependent Insulinotropic Polypeptide) — now, in 2025, well-known from the drugs that mimic the action of these natural hormones, to treat type 2 diabetes and obesity, by stimulating insulin release, improving blood sugar control, and reducing appetite.

May 1992 we were the first to show direct potentiation of the insulin response from isolated islets by (near-)physiological doses of both the gut hormones GLP-1 and GIP during perifusion (in vitro) of canine islets at 7.5-10 mM glucose levels (1,2), and January 1994 we next confirmed the incretin effect of a (near-)physiological dose of GLP-1 after canine islet transplantation , in a poster presented at the 13th Workshop of the AIDSPIT (aka AIDPIT) Study Group , Igls, Austria, January 23–25, 1994 (3).

Background of the work was posted earlier this year (4) and the work was published Nov 1994 in my PhD thesis Pancreatic islet transplantation , available online (5).

Welcome Reception 5th IPITA on Sunday 18 June 1995, at the congress venue , the Eden Roc Resort Hotel, in Miami Beach, FL (USA) • Michel van der Burg • Miracles.Media • @1MEMO 20251204_2 • TakeNode 8eb04df1-8280-4de3-a206-4b63413c3025

June 1995, 5th IPITA Talk

Tuesday June 20, 1995 at 10:30, at the 5th International Congress on Pancreas and Islet Transplantation (IPITA), in Miami Beach, FL (USA), was my first talk at a world congress presenting the outcome of all our detailed metabolic studies in the canine islet transplants , including the incretin effect of GLP-1, and also our findings with isolated canine islets during perifusion, in vitro, of the direct insulinotropic effects of the gut hormones GIP and GLP-1 (6) . That slideshow presentation (digitally remastered*) is posted here (1MEMO 20251204_1).

The proceedings paper delivered at the 5th IPITA is included in the Notes (7) , as well as the referenced previous papers (8,9)

Notes

1. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Functie na intralienale transplantatie van eilandjes van Langerhans in de hond ( Function after intrasplenic transplantation of islets of Langerhans in dogs ). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992. In : Gut factors controlling pancreatic islets • 20240910 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/09/10/gut-factors-controlling-pancreatic-islets-20240910/

2. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

3. Metabolic control after canine islet transplantation. M.P.M. van der Burg, P.T.R. van Suylichem, O.R. Guicherit, J.H.M. van Deijnen, M. Frölich, and H.G. Gooszen (Universities of Leiden & Groningen, The Netherlands). Poster presented at the 13th Workshop of the AIDSPIT Study Group , Igls, Austria, January 23–25, 1994 • In : GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • Michel van der Burg • Miracles.Media • @1MEMO 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • URL https://michelvanderburg.com/2025/11/14

4. Background of this work was posted in Dutch Islets Transplant Team • @1MEMO 20250125 • URL https://michelvanderburg.com/2025/01/25/dutch-islets-transplant-team-1memo-20250125/

5. Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Dec 4th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .

6. Oral presentation : Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. 5th International Congress on Pancreas and Islet Transplantation (IPITA), Miami Beach (USA) June 20, 1995.

7. 5th IPITA Proceedings publication : Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. Transplant Proc. 1995 Dec;27(6):3187-8. PMID: 8539903. PDF available below PMID_8539903

PMID_8539903 Download

Referenced previous publications :

8. Van der Burg MPM, Gooszen HG. The metabolic efficiency of islet grafts: an overview. In: Islet transplantation — current status of clinical application and experimental results. Hesse UJ , Pichlmaier H, Eds., Lengerich Germany, Wolfgang Pabst Verlag, 1992, p.93–99. Pabst hash code 0383b3abad3ae774b146ea0425c2227a PDF available below 1MEMO_20251204_4

1MEMO_20251204_4 Download

9. Van der Burg MPM, Guicherit OR, Scherft JP, Lemkes HHPJ, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors (Abstract). Neth J Med 1992; 41: A25. PDF available below 1MEMO_20251204_3

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Other previous publications :

10. Van der Burg MPM, Guicherit OR, Van Suylichem PTR, Frölich M, Van Deijnen JHM, Gooszen HG. Importance of the entero-insular axis for islet graft function (Abstract). Cell Transplant 1994; 3: 257. Abstract – Second International Congress of the Cell Transplant Society May 1–4, 1994 Hyatt Regency Hotel • Minneapolis, Minnesota . https://doi.org/10.1177/096368979400300302 (Abstracts published , posters not presented). PDF available below 10.1177_096368979400300302

10.1177_096368979400300302 Download

11. Chapter 8, Doctoral Thesis, Leiden University, November 1994 : Metabolic control after intrasplenic islet autotransplantation in dogs: ß-cell secretory capacity, insulin action, and the enteroinsular axis. Michael P.M. van der Burg, Paul T.R. van Suylichem, Onna R. Guicherit, Marijke Frölich, Herman H.P.J. Lemkes, and Hein G. Gooszen. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 8). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .
Direct link to file Chapter 8 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486615/view .

12. Doctoral Thesis, Leiden University, November 1994 (Chapter 6) : lnsulinotropic properties of cholecystokinin, gastric inhibitory polypeptide, and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Michael P.M. van der Burg, Onna R. Guicherit, Marijke Frölich and Hein G. Gooszen. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 6). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .
Direct link to file Chapter 6 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486613/view

Full papers

13. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

14. Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glucoregulation after canine islet transplantation: contribution of insulin secretory capacity, insulin action, and the entero-insular axis. Cell Transplant. 1997 Sep-Oct;6(5):497-503. doi: 10.1177/096368979700600509. PMID: 9331501. PDF link https://pure.rug.nl/ws/portalfiles/portal/58359942/096368979700600509.pdf

PDF (CC-BY-NC) from publisher via ResearchGate – https://www.researchgate.net/publication/317805208_Glucoregulation_after_Canine_Islet_Transplantation_Contribution_of_Insulin_Secretory_Capacity_Insulin_Action_and_the_Entero-Insular_Axis

* 1MEMO 20251204_1 . Digitally remastered presentation, using the images from the original slides handout, and the handout of the text spoken during the original presentation , now read using my personal synthesized voice.

#GLP1 #GIP #diabetes #islets #transplant

Updates

Update 20260203 – added ResearchGate link ref 14

Citation info : Glycemic control mechanisms after canine islet autografting • IPITA 1995 • Michel van der Burg • Miracles.Media • @1MEMO 20251204 • URL https://michelvanderburg.com/2025/12/04