Tag Archives: event

Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients


Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients.
Here a film made of my ‘powerpoint’ presentation Feb 22, 2000 – on behalf of our Surgical Department – at the Opening Symposium of the Interdivisional GMP (‘Good Manufacturing Practice’) facility of the Leiden University Medical Centre – the LUMC in Leiden, Holland.

Background
I first started – with a background in biochemistry and philosophy – some 20 years earlier (from 1981) research at the Department of Surgery of the Leiden University Hospital (AZL) in the field of transplantation of the pancreas organ to cure diabetes.

Succesful Experiment Islet Transplantation
From 1986 my focus shifted to innovative procedures for the isolation out of the pancreas organ, of the ca 1 million islets of Langerhans – the insulin producing cell clumps with a size between ca 0.1 to 1 mm , that is 0.004 to 0,04 inch.
In 1989 we performed the first successful autologous transplantations in Holland (in collaboration with the Minneapolis Islet Lab with Jane Field and David Sutherland) – for the first time world-wide with pure islets using our novel procedure of using the organ preservation UW solution (University of Wisconsin / collaboration with dr Robert Carter , Du Pont, UK) during isolation in a pre-clinical large animal diabetes model – with long term transplant functioning with near-normal blood sugar regulation.

First series of human islet isolations , 1989-1990
Next in 1989-1990 we performed a first series of islet isolations from human donor pancreases using our novel techniques of isolation and purification in UW organ preservation solution – in association with a European Concerted Action for the Treatment of Diabetes – the Brussels headed (Daniel Pipeleers) ‘Multicenter program on the treatment of diabetes by islet cell transplantation’.

Exploring human islet work
Subsequently – after some years of exploring options for animal to human islet transplantation , using pig pancreas islets – so-called xenotransplantation – I was asked in 1997 to explore the requirements for operationalization of clinical islet transplantation in the LUMC. December 1997 our Surgical Department decided to go for it.

Establishing the human islet isolation laboratory in Leiden.
In 1998 after a month working in dr Camillo Ricordi’s Diabetes Research Institute in Miami, I started a large series of human islet isolation procedures in Leiden, and from september 1998 documenting the detailed GMP (‘Good Manufacturing Practice’) standard operating procedures, as well as ‘building’ our clinical islet isolation clean room with help from the Miami team in close cooperation with dr. Amon Wafelman (department of Clinical Pharmacy & Toxicology) managing the building of our brand new Interdivisional GMP-facility LUMC (IGFL) of six clean rooms and two chemical product laboratories – that was opened officially Feb 22, 2000 with the symposium, a film, and a tour in the GMP facility.
One year later the GMP islet facility was fully operational and summer 2001 we got a cautious green light (GMP license) for clinical transplantation of any high quality – approved – islet preparations.
Around the same time islet transplantation suddenly became very promising with ‘The Edmonton protocol’. However with the fast increase of logistic and financial demands, and reorganization between LUMC divisions it took till 2007 to finally start first transplants by an expanded islet team in the LUMC clinical islet center in Leiden.
Worldwide since 2000 several hundred people have received islet transplants that generally do not result in long term insulin independence , but do help sugar regulation in the transplant recipients.

Notes :
Links to some publications, news paper reports etc will follow later.

Updates:

Post updated with more details 20191215

Presentation ‘Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients’ by Michel van der Burg, Feb 22, 2000 – Opening Symposium of the Interdivisional GMP facility of the Leiden University Medical Centre, Leiden, Netherlands. Film : 20191213 Michel van der Burg | miracles.media

ESPACE Simon GRONOWSKI

ESPACE Simon GRONOWSKI inauguration 19-02-2019 , Saint-Sépulcre S2J, Liège (BE) ① memo 20190220 ~ ESPACE Simon GRONOWSKI ~ Miracles.Media

Peter Wein’s Broadcast Interview Filmmaker Richard Bloom


① memo 20181118 ~ Premiere Robert Clary Story ~ Interview (segment shown here) of filmmaker Richard Bloom on his documentary From Buchenwald To Hollywood, The Robert Clary Story by Peter Wein (Internet Radio’s WEI Network – http://www.WEInetwork.com ) and guest co-host Anne Butters – in a live broadcast from the FT. Lauderdale International Film Festival on Saturday Nov. 10th , at the Savor Cinema in Fort Lauderdale , Florida , USA.
Watch the entire archived interview at Peter Wein’s facebook here https://www.facebook.com/peter.wein.16/videos/10215054282042911/
Thank you Peter Wein for providing your audio file that I synced in post in this short film with a segment of your video stream.
More on The Robert Clary Story here https://michelvanderburg.com/2018/09/23/robert-clary-story-world-premiere-13-nov-2018-in-hollywood-fl/ ———————

Premiere Robert Clary Story ~ Hollywood, Florida

① memo 20181113 ~ Premiere Robert Clary Story ~ Hollywood, Florida
More here https://michelvanderburg.com/2018/09/23/robert-clary-story-world-premiere-13-nov-2018-in-hollywood-fl/

Opening Night Fort Lauderdale International Film Festival

Last Friday Opening Night Fort Lauderdale International Film Festival interview Richard Bloom on screening The Robert Clary Story from around 13th minute mark – link :

https://www.facebook.com/FortLauderaleInternationalFilmFestival/videos/1965691876801249/

BELLA CIAO … Transport XX

Now also live on YouTube.

The Crescendo choir (conductor : Urbain Van Asch) sings Bella Ciao during today’s Transport XX commemoration in Boortmeerbeek , Belgium
① memo 20160417 ~ Film : Michel van der Burg – michelvanderburg.com | 1-memo.com

Transport XX – herdenking 2016

Transport XX herdenking 2016 – “Een radicale keuze…”  – Update April 22
① memo 20160419 ~ Radical Choice … Transport XX” ~ 2016 commemoration , theme “A radical choice…” in Boortmeerbeek , Belgium , April 17, 2016 .
Video online 19 April 2016.
UPDATE video 22 April 2016.
Film : Michel van der Burg – michelvanderburg.com | 1-memo.com .