Islet Isolation Assessment • 4th IPITA Amsterdam 1993 • @1MEMO 20260201

4th IPITA , Amsterdam, 1993 • Michel van der Burg • Miracles•Media • 1MEMO_20260201_11 • TakeNode 1c776460-e3a6-46df-8b8b-f43f30517021

Slideshow (handout slides, partially remastered) of my talk titled ‘Assessment of islet isolation efficacy in dogs’ presented at the 4th International Congress on Pancreas and Islet Transplantation (4th IPITA), Amsterdam (The Netherlands) June 27–30, 1993 • Michel van der Burg • Miracles•Media • 1MEMO_20260201_1 • TakeNode 628b273c-b8e1-4e23-8f8b-1550c7960cb3 • PDF available below.

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Text of talk ‘Assessment of islet isolation efficacy in dogs’ presented at the 4th International Congress on Pancreas and Islet Transplantation (4th IPITA), Amsterdam (The Netherlands) June 27–30, 1993 • Michel van der Burg • Miracles•Media • 1MEMO_20260201_12 • TakeNode 9bc525ba-dfdb-4d92-8af4-bdd21ee1faaf • PDF available below.

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The proceedings paper delivered at the 4th IPITA is included below in the Notes , as well as references of the related full papers and related chapters of PhD thesis.

This IPITA meeting in Amsterdam was also a good opportunity to catch up with colleague Nick London (see photo) from the Leicester (UK) group discussing our Leiden and Leicester islet isolation work using the University of Wisconsin organ preservation solution (UWS). The Leicester group were one of the first groups to examine and adopt our novel method of islet isolation in UWS.

Several groups reported at this 4th IPITA symposium on the effects of using (modified) UW solution (UWS) during islet isolation : Leicester (pig, human, islets), Oxford (rat, rabbit islets), Minneapolis (human islets), Paris (pig islets), and Giessen (clinical human allo islet transplantation).

Michel van der Burg (Leiden) and Nick London (Leicester) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Matthias Ohimer , Michel van der Burg • Miracles•Media • @1MEMO_20260201_3 • TakeNode c4451ac4-84fc-4068-823a-b475dc0bb35e • #islets #LUMC #transplant #IPITA #diabetes #Amsterdam #Leicester #Leiden

During the 4th IPITA, I discussed in detail the rationale of our UWS work, the important components of UWS, and basal solution for islet purification (Percoll-UWS) with Yoko Mullen (in particular) and her co-worker Charles Brunicardi (see photo) of the UCLA Islet Transplant Team (LA, USA)…contributing to the development of Yoko’s Los Angeles preservation solution #1 (LAP-1) patented in 1997 (see Notes, Patent US5679565A).

Michel van der Burg (Leiden), Charles Brunicardi (LA), and (? Norm Kneteman, Edmonton) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Matthias Ohimer , Michel van der Burg • Miracles•Media • @1MEMO_20260201_4 • TakeNode 93930fd4-ee21-4b18-90ca-83e1731102e1 • #islets #LUMC #transplant #IPITA #diabetes #Amsterdam #Leiden #LosAngeles #LA #Edmonton

The official founding meeting of the IPITA was actually at this 4th International Congress on Pancreas and Islet Transplantation — June 27–30, 1993 in Amsterdam (The Netherlands) hosted by Dr. Reinout van Schilfgaarde (see photo).

Reinout van Schilfgaarde (Groningen) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Matthias Ohimer , Michel van der Burg • Miracles•Media • @1MEMO_20260201_5 • TakeNode 9a713895-1dc6-462a-9621-1160ddeb2d8e • #islets #LUMC #transplant #IPITA #diabetes #Amsterdam #Leiden

Below some of the photos I took during a break at the 4th IPITA in Amsterdam, near the Rijksmuseum (corner Spiegelgracht / Tweede Weteringdwarsstraat ) , and (Jane Field) at the congress venue (RAI Amsterdam).

Garth Warnock (Edmonton), Bernhard Hering (Giessen), Nick London (Leicester) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Michel van der Burg • Miracles•Media • @1MEMO_20260201_6 • TakeNode 4a31bed0-8308-4a51-9291-06d53549ef3d • #islets #LUMC #transplant #IPITA #diabetes #Amsterdam #Leiden #Edmonton #Giessen #Leicester

Garth Warnock (Edmonton), Bernhard Hering (Giessen) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Michel van der Burg • Miracles•Media • @1MEMO_20260201_8 • TakeNode ffbe0d97-be31-4dc9-bc94-9e2b708ccb47 • #IPITA #Amsterdam #islets #LUMC #transplant #diabetes #Edmonton #Giessen #Leiden

Jane Field (Minneapolis) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Michel van der Burg • Miracles•Media • @1MEMO_20260201_9 • TakeNode d1921eb6-2c38-49c2-9693-d288d940268d • #IPITA #Amsterdam #islets #LUMC #transplant #diabetes #Minneapolis #Leiden

Notes

Oral presentation 4th IPITA : Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Prins FA, Bruijn JA, Gooszen HG. Assessment of islet isolation efficacy in dogs. 4th International Congress on Pancreas and Islet Transplantation, Amsterdam (The Netherlands) June 27–30, 1993.

Oral presentation (selection of slides Assessment of islet isolation efficacy in dogs). Van der Burg MPM. Workshop: Guidelines for the standardized assessment of retrieval and transplantation of islets. 4th International Congress on Pancreas and Islet Transplantation, Amsterdam (The Netherlands) June 27–30, 1993.

Proceedings paper 4th IPITA : Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Prins FA, Bruijn JA, Gooszen HG. Morphometry of native and isolated islets: a new approach to isolation assessment. Transplant Proc. 1994 Apr;26(2):632-3. PMID: 7513459 . PDF available below

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This slideshow had been presented earlier that year with a different title at the 12th Workshop of the AIDSPIT Study Group in Igls :
Oral presentation 12th AIDSPIT : Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Bruijn JA, Prins FA, Gooszen HG. Quantification of islet isolation efficiency in dogs. 12th Workshop of the AIDSPIT Study Group of the European Association for the Study of Diabetes, Igls (Austria) January 24–26, 1993.

Title Slide (remastered) 12th AIDSPIT, Igls (Austria), 1993 • Michel van der Burg • Miracles•Media • 1MEMO_20260201_2 • TakeNode 2cfe23eb-cbb0-4942-afcb-56755ebcb467

Abstract 12th AIDSPIT : Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Bruijn JA, Prins FA, Gooszen HG. Quantification of islet isolation efficiency in dogs (Abstract). Horm Metab Res 1993; 25: 52. 1MEMO_20260201_10 PDF available below.

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Corresponding Full Papers (Thesis chapters)

Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Bruijn JA, Gooszen HG. Impact of donor-related variables on islet isolation outcome in dogs. Diabetologia. 1994 Jan;37(1):111-4. doi: 10.1007/BF00428786. PMID: 8150223 . Article PDF Free download.

Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Prins FA, Bruijn JA, Gooszen HG. Assessment of islet isolation efficacy in dogs. Cell Transplant. 1994 Jan-Feb;3(1):91-101. doi: 10.1177/096368979400300113. PMID: 8162296

Chapters (2,3) Doctoral Thesis (corresponding with full papers) : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Dec 4th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 . PDF free download available via link.

Patent US5679565

Method of Preserving Pancreatic Islets , Mullen et al. United States Patent US5679565 . Download PDF https://patentimages.storage.googleapis.com/80/60/f6/744731dd250c48/US5679565.pdf

Online history of patent :
US5679565A – Method of preserving pancreatic islets – Google Patents URL https://patents.google.com/patent/US5679565A

Portfolio

Online Portfolio – Science : https://michelvanderburg.com/category/science/

#islet #IPITA #AIDPIT #Amsterdam #Igls #Innsbruck #UWS #density #purification #history #AIDSPIT #Leiden #Leicester #Netherlands #Austria #Langerhans #isolation #transplantation #pancreas #diabetes #insulin #donor #assessment #michelvanderburg #symposium #workshop #science #EPITAsymposium #EASD #ESOT #Edmonton #Giessen #Minneapolis

Citation info : Islet Isolation Assessment • 4th IPITA Amsterdam 1993 • Michel van der Burg • Miracles•Media • @1MEMO 20260201 • URL https://michelvanderburg.com/2026/02/01

24th AIDPIT Workshop Igls • @1MEMO 20260125

Short impression of the 24th Workshop of the AIDPIT Study Group (Artificial Insulin Delivery, Pancreas and Islet Transplantation), in Igls (Innsbruck) Austria, 23-25 January 2005.

Video from January 25th featuring :

(i) the Igls Congress park ;

(ii) the morning opening lecture by Mathias Brendel, with chairs Bart Keymeulen (Brussels, Belgium) and Mathias Brendel (Giessen, Germany), and, in the lecture hall : president Annika Tibell (Sweden) and honorary member Reinhard Bretzel (Germany) ;

(iii) a lunch time meeting with José Oberholzer (Chicago, USA) … not familar yet 😉 with that new little Sony T1 camera with a periscopic lens inside .. ;

(iv) one of the afternoon discussion groups (Clinical Islet Transplantation) ;

(v) clips from the plenary oral abstract session with chairs Karin Ulrichs (Germany) and Richard Smith (Bristol , UK) and lectures by Norma Sue Kenyon (Miami , USA) and Piero Marchetti (Pisa, Italy) with discussion by Daniel Brandhorst.

Notes

AIDPIT was known first as AIDSPIT, short for : Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation.
The AIDSPIT study group of the European Association for the Study of Diabetes (EASD) was established in the early 1980s.
In 2000 the acronym AIDSPIT was replaced by ‘AIDPIT’, in order to avoid confusion with the AIDS disease.

The annual meetings are generally held in the little town of Igls, close to Innsbruck in Austria — the first years, early in February, and next, late in January. Once, in the 1990s (following a discussion ‘…why always in Igls?’, the meeting was held in Amsterdam.
From 2011, the annual workshops of AIDPIT continued as a joined meeting with the EPITA — the European Pancreas and Islet Transplant Association — part of the European Society for Organ Transplantation (ESOT).
From 2024 — following the 12th EPITA Symposium & 41st AIDPIT Workshop in 2023 — this symposium is referred to as the EPITA Symposium.

The original film (2016) was uploaded to Vimeo (size 640×480), and posted Jan 25, 2016 (20160125) at blogging site 1-memo.com , URL https://1-memo.com/2016/01/25/24th-aidpit-workshop-igls/

A second, remastered, video edition (2022) was produced by upscaling the original video file (converted to size 1920 × 1440) and adding a caption, and uploaded to YouTube Jan 25, 2022.

This second edition is published now – Jan 25th 2026 – as part of the Science series of posts at my site michelvanderburg.com, started in 2022 to make available online a portfolio of the science section of my CV, a project I hope to finish soon… in a few months.

Portfolio – Science : https://michelvanderburg.com/category/science/

#AIDPIT #EPITAsymposium #Igls #Innsbruck #Tyrol #history #ESOT_EPITA ##AIDSPIT #Austria #islet #Langerhans #isolation #transplantation #pancreas #diabetes #insulin #michelvanderburg #symposium #EASD #ESOT #workshop #EPITA #science

Citation info : 24th AIDPIT Workshop Igls • Michel van der Burg • Miracles•Media • @1MEMO 20260125 • TakeNode 3baced38-ee79-4127-932d-0136293529e7 • URL https://michelvanderburg.com/2026/01/25

Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients

Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients.
Here a film made of my ‘powerpoint’ presentation Feb 22, 2000 – on behalf of our Surgical Department – at the Opening Symposium of the Interdivisional GMP (‘Good Manufacturing Practice’) facility of the Leiden University Medical Centre – the LUMC in Leiden, Holland.

Background
I first started – with a background in biochemistry and philosophy – some 20 years earlier (from 1981) research at the Department of Surgery of the Leiden University Hospital (AZL) in the field of transplantation of the pancreas organ to cure diabetes.

Succesful Experiment Islet Transplantation
From 1986 my focus shifted to innovative procedures for the isolation out of the pancreas organ, of the ca 1 million islets of Langerhans – the insulin producing cell clumps with a size between ca 0.1 to 1 mm , that is 0.004 to 0,04 inch.
In 1989 we performed the first successful autologous transplantations in Holland (in collaboration with the Minneapolis Islet Lab with Jane Field and David Sutherland) – for the first time world-wide with pure islets using our novel procedure of using the organ preservation UW solution (University of Wisconsin / collaboration with dr Robert Carter , Du Pont, UK) during isolation in a pre-clinical large animal diabetes model – with long term transplant functioning with near-normal blood sugar regulation.

First series of human islet isolations , 1989-1990
Next in 1989-1990 we performed a first series of islet isolations from human donor pancreases using our novel techniques of isolation and purification in UW organ preservation solution – in association with a European Concerted Action for the Treatment of Diabetes – the Brussels headed (Daniel Pipeleers) ‘Multicenter program on the treatment of diabetes by islet cell transplantation’.

Exploring human islet work
Subsequently – after some years of exploring options for animal to human islet transplantation , using pig pancreas islets – so-called xenotransplantation – I was asked in 1997 to explore the requirements for operationalization of clinical islet transplantation in the LUMC. December 1997 our Surgical Department decided to go for it.

Establishing the human islet isolation laboratory in Leiden.
In 1998 after a month working in dr Camillo Ricordi’s Diabetes Research Institute in Miami, I started a large series of human islet isolation procedures in Leiden, and from september 1998 documenting the detailed GMP (‘Good Manufacturing Practice’) standard operating procedures, as well as ‘building’ our clinical islet isolation clean room with help from the Miami team in close cooperation with dr. Amon Wafelman (department of Clinical Pharmacy & Toxicology) managing the building of our brand new Interdivisional GMP-facility LUMC (IGFL) of six clean rooms and two chemical product laboratories – that was opened officially Feb 22, 2000 with the symposium, a film, and a tour in the GMP facility.
One year later the GMP islet facility was fully operational and summer 2001 we got a cautious green light (GMP license) for clinical transplantation of any high quality – approved – islet preparations.
Around the same time islet transplantation suddenly became very promising with ‘The Edmonton protocol’. However with the fast increase of logistic and financial demands, and reorganization between LUMC divisions it took till 2007 to finally start first transplants by an expanded islet team in the LUMC clinical islet center in Leiden.
Worldwide since 2000 several hundred people have received islet transplants that generally do not result in long term insulin independence , but do help sugar regulation in the transplant recipients.

Notes :
Links to some publications, news paper reports etc will follow later.

Updates:

Post updated with more details 20191215

Presentation ‘Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients’ by Michel van der Burg, Feb 22, 2000 – Opening Symposium of the Interdivisional GMP facility of the Leiden University Medical Centre, Leiden, Netherlands. Film : 20191213 Michel van der Burg | miracles.media