PentaStarch UWS Pancreatic Islet Purification • 20240802

Following first introduction of the use UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options.
One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS (7) . Note that Pentastarch is a component of UWS.

The Percoll-UWS poster was posted recently (8)
Here the remastered poster presentation on Pentastarch-UWS :
Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 3 strips) were then glued on site along the perforation, creating the 36/24 inch poster.
Finally the photo print of the purified islets was pasted in the poster at the spot at the venue in Lyon. In Lyon a photo of a dithizone stained Pentastarch purified islet sample was shown. To save time the archive was not searched for that image, and another image of Pentastarch purified islets was inserted in this remastered edition, a reflection contrast micrograph of an ultrathin section of Pentastarch purified islets immuno-stained with gold on insulin in the islet cells.

Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla and now learned to use the app to further correct most conversion issues. Pixelmator Pro was used for additional ‘repair’ and insertion of the photo scan.

Notes

1. Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2022/08/22/minneapolis-1989-poster-uw-solution-islet-isolation-20220822/

2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

6. Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media | URL https://michelvanderburg.com/2022/11/25/pancreatic-islet-transplantation-thesis-repository-leiden-university-20221125/

7. Pentastarch is the synthetic colloid component of UWS (ViaSpan / Belzer solution), a purified fraction (250 kD) of hydroxyethyl starch. Pentastarch powder was used here to increase the Pentastarch concentration and density of the UWS.

8. Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com

Citation info : PentaStarch UWS Pancreatic Islet Purification • 20240802 | Michel van der Burg | michelvanderburg•com | TakeNode d11f1cef-a343-4466-8814-3e52a0261cae

Percoll UWS Pancreatic Islet Purification • 20240731

Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e

Following first introduction of the use of UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options.
One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS . Note that Pentastarch is a component of UWS.

Here one of these poster presentations, on Percoll-UWS :
Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application, inserted with a bar graph prepared in the Cricket Graph app.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 5 strips) were then glued on site along the perforation, creating a 36/41 inch poster.
Finally the RCM photo print of the purified human islets was pasted in the poster at the spot at the venue in Lyon.

Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla (open source app) with nice results. Only minor issues appeared with text spacing of the top row of the table , and the caption in the photo section. Some color is showing in the graphs , not evident on my grayscale equipment in the 90s. …. The poster image of 20240731 has been replaced by a remastered image , see Note Update 20240808 below.

Notes

Update 20240808

Remastered poster ‘Lyon 1991 Percoll Poster | 20240731 • 20240808’

Following the first publication here last month (20240731) of the original 1991 MacDraw poster opened (converted) in LibreOffice Vanilla (1), I learned how to do some editing in the LibreOffice app to further restore poster layout details such as line spacing, and font style. An archive photo of another poster presentation in Pittsburgh in 1992 (that will be posted here soon) guided this restoration.

Current workflow :
1) opening the MacDraw II file in LibreOffice Vanilla
2) editing in in LibreOffice Vanilla , saving as LibreOffice Vanilla file (OpenDocument Drawing – extension odg).
3) opening the photo tiff image in the Mac’s Preview app, and copying the displayed image to the clipboard
4) pasting the clipboard image in the LibreOffice doc , and dragging to adjust size and location
5) exporting the file as PDF (image resolution 300 DPI ; PDF/A-1b ; PDF/UA).
6) Converting the PDF in the Preview app to TIFF 144 pixels/inch.
7) Adding caption , metadata etc with JPEG conversion in PhotoMill.

This 20240731 post now has this updated 20240808 – poster image.

Citation info : Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com

Lancet Letter 1989 UW Solution Islet Isolation | 20220823

Lancet Letter 1989 UW Solution Islet Isolation | 20220823 | Michel van der Burg | michelvanderburg•com | June 1989 preparing first submission for publication as letter in the Lancet medical journal the new concept of using the UW solution ( the University of Wisconsin organ preservation solution) for isolation and complete purification of islets of Langerhans in the laboratory for transplantation in diabetic patients. Later that year that exciting discovery was presented at meetings in Minneapolis, USA, and Barcelona, Spain. Details in yesterday’s post. Submission for publication as a letter in the Lancet had had no succes 😉
Final draft text produced on my personal Macintosh ED and Imagewriter-II matrix printer with Geneva font.

TakeNode ID: 9f11f523-9b5f-47e8-be99-b242a1ba9b40

TAGS #Lancet #journal #UWS #UW #solution #preservation #organ #islet #isletofLangerhans #Langerhans #pancreas #poster #concept #purification #transplantation #congress #meeting #Minneapolis #Netherlands #Leiden #LUMC #AZL #Barcelona #Spain #LibreOffice #Vanilla #MacDraw #MacintoshED #512K #Imagewriter #TakeNode #1Memo #michelvanderburg

Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822

Minneapolis 1989 Poster • 20240804 • michelvanderburg•com

September 1989 at a symposium of two international societies in Minneapolis, USA (1) , I presented this first poster on the ‘ invention ‘ of using the UW solution ( the University of Wisconsin organ preservation solution) during pancreatic islet isolation, with the exciting discovery of near-complete purification of islets of Langerhans in the laboratory for transplantation in diabetic patients.

The next month at a meeting in Barcelona, Spain, this rather radical new concept was introduced (2, 3). June 1989 submission for publication as a letter in the Lancet had had no succes 😉

Poster designed and produced on my personal Macintosh ED and Imagewriter-II matrix printer, allowing full width printing over multiple pages in landscape (a low cost do-it-yourself discovery by me, at a time when posters were prepared on poster boards by graphical professionals using photography and special lettering techniques for around $400). Original MacDraw II file conversion with LibreOffice Vanilla.

Notes

1) Van der Burg MPM, Gooszen HG, Ploeg RJ, Scherft JP, Field MJ, Guicherit OR, Terpstra JL, Van de Woude FT, Frölich M, Bruijn JA. Comparison of islet isolation techniques in dogs: Over 90% purified islets using UW solution. 4th International Symposium on Organ Procurement and Preservation and 2nd International Congress on Pancreatic and Islet Transplantation, Minneapolis (USA) September 17–22, 1989.

2) Van der Burg MPM, Gooszen HG, Ploeg RJ, Guicherit OR, Scherft JP, Terpstra JL, Bruijn JA, Frölich M. Pancreatic islet isolation with UW solution: a new concept. 4th Congress of the European Society for Organ Transplantation, Barcelona (Spain) November 1–4, 1989.

3) Van der Burg MPM, Gooszen HG, Ploeg RJ, Guicherit OR, Scherft JP, Terpstra JL, Bruijn JA, Frölich M. Pancreatic islet isolation with UW solution: a new concept. Transplant Proc 1990; 22: 2050–2051.

Updates

20240804 – This remastered 20240804 edition of the Minneapolis 1989 poster , now shows the original photo of the islet prep as posted at the meeting in 1989. In addition the correct line spacing is now restored.
The previous poster published online two years ago (20220822) showed a different photo print (higher magnification, and without the bar indicator) of the same islet prep, not the correct image.

20241211 – Minor text updates

Citation info : Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | TakeNode 91d70715-3fb3-4380-906a-0daf1b5ddd6a

Floaters

Floaters ~ One minute at the border of the Silent Rhine from under the ‘Fish-bridge’ at the confluence of the Old and New Rhine, where Leyden was born, October 2009, Leyden, Holland.
First published in 2010 (20101023) via my now obsolete YouTube channel iClip , as : 1’2009 Stille Rijn – Leiden – Holland.
Next remade and submitted as ‘floaters’ for The One Minutes Collection (# 3877 , file mvdb20120829_floaters ) August 31, 2012. That version upscaled for today ‘s online version here.
Film : Floaters (20101023 – 20120831 – 20200513) Michel van der Burg | michelvanderburg.com | miracles.media

Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients

Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients.
Here a film made of my ‘powerpoint’ presentation Feb 22, 2000 – on behalf of our Surgical Department – at the Opening Symposium of the Interdivisional GMP (‘Good Manufacturing Practice’) facility of the Leiden University Medical Centre – the LUMC in Leiden, Holland.

Background
I first started – with a background in biochemistry and philosophy – some 20 years earlier (from 1981) research at the Department of Surgery of the Leiden University Hospital (AZL) in the field of transplantation of the pancreas organ to cure diabetes.

Succesful Experiment Islet Transplantation
From 1986 my focus shifted to innovative procedures for the isolation out of the pancreas organ, of the ca 1 million islets of Langerhans – the insulin producing cell clumps with a size between ca 0.1 to 1 mm , that is 0.004 to 0,04 inch.
In 1989 we performed the first successful autologous transplantations in Holland (in collaboration with the Minneapolis Islet Lab with Jane Field and David Sutherland) – for the first time world-wide with pure islets using our novel procedure of using the organ preservation UW solution (University of Wisconsin / collaboration with dr Robert Carter , Du Pont, UK) during isolation in a pre-clinical large animal diabetes model – with long term transplant functioning with near-normal blood sugar regulation.

First series of human islet isolations , 1989-1990
Next in 1989-1990 we performed a first series of islet isolations from human donor pancreases using our novel techniques of isolation and purification in UW organ preservation solution – in association with a European Concerted Action for the Treatment of Diabetes – the Brussels headed (Daniel Pipeleers) ‘Multicenter program on the treatment of diabetes by islet cell transplantation’.

Exploring human islet work
Subsequently – after some years of exploring options for animal to human islet transplantation , using pig pancreas islets – so-called xenotransplantation – I was asked in 1997 to explore the requirements for operationalization of clinical islet transplantation in the LUMC. December 1997 our Surgical Department decided to go for it.

Establishing the human islet isolation laboratory in Leiden.
In 1998 after a month working in dr Camillo Ricordi’s Diabetes Research Institute in Miami, I started a large series of human islet isolation procedures in Leiden, and from september 1998 documenting the detailed GMP (‘Good Manufacturing Practice’) standard operating procedures, as well as ‘building’ our clinical islet isolation clean room with help from the Miami team in close cooperation with dr. Amon Wafelman (department of Clinical Pharmacy & Toxicology) managing the building of our brand new Interdivisional GMP-facility LUMC (IGFL) of six clean rooms and two chemical product laboratories – that was opened officially Feb 22, 2000 with the symposium, a film, and a tour in the GMP facility.
One year later the GMP islet facility was fully operational and summer 2001 we got a cautious green light (GMP license) for clinical transplantation of any high quality – approved – islet preparations.
Around the same time islet transplantation suddenly became very promising with ‘The Edmonton protocol’. However with the fast increase of logistic and financial demands, and reorganization between LUMC divisions it took till 2007 to finally start first transplants by an expanded islet team in the LUMC clinical islet center in Leiden.
Worldwide since 2000 several hundred people have received islet transplants that generally do not result in long term insulin independence , but do help sugar regulation in the transplant recipients.

Notes :
Links to some publications, news paper reports etc will follow later.

Updates:

Post updated with more details 20191215

Presentation ‘Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients’ by Michel van der Burg, Feb 22, 2000 – Opening Symposium of the Interdivisional GMP facility of the Leiden University Medical Centre, Leiden, Netherlands. Film : 20191213 Michel van der Burg | miracles.media

Paul Verhoeven : Jesus The Man

PAUL VERHOEVEN : JESUS THE MAN

NL – 10 jaar geleden – dinsdag 31 maart 2009 – kwam regisseur en schrijver Paul Verhoeven op uitnodiging van het Instituut der Godsdienstwetenschappen van de Leidse universiteit naar Leiden , om te praten over zijn boek ‘Jezus van Nazaret’ …. ‘een realistisch portret’ dat eind 2008 was uitgegeven (geschreven in samenspraak met Rob van Scheers).
Naast cinema kent Nederlands grootste filmregisseur Paul Verhoeven nóg een levenslange fascinatie: Jezus van Nazareth. Na zijn vertrek uit Nederland in 1985 meldde hij zich daarom in Californië aan bij het zogeheten Jesus Seminar, een hoog aangeslagen wetenschappelijke denktank van zo’n zeventig eminente professoren in godgeleerdheid, filosofie, linguïstiek en bijbelgeschiedenis, opgericht door Amerika’s meest vooraanstaande liberale exegeet Robert W. Funk.
Als enige niet-theoloog mocht Verhoeven aanschuiven bij de discussies die tot doel hadden de historische figuur Jezus van de mythische bijbelfiguur Jezus te bevrijden, hem te ontdoen van tweeduizend jaar christelijke inkleuring. Als bijdrage aan dit onderzoek schreef Paul Verhoeven – in Leiden afgestudeerd in wis- en natuurkunde – vele wetenschappelijke papers die vervolgens de basis vormden voor zijn spraakmakende boek Jezus van Nazaret – inmiddels in meerdere vertalingen uitgegeven .
Een discussie met Paul Verhoeven in een panel met Prof. Jürgen Zangenberg, dr Wim Hofstee, en studenten – met een introductie door dr Peter Verstraten – in de Lokhorstkerk te Leiden.
Een reportage van 45 min beeld van de 2 uur durende discussie – gefilmd met een Sony T500 compact camera.
NB : Een deel (ca 17 min) van deze reportage verscheen eerder, 3 april 2019, in een post getiteld ‘JESUS THE MAN – FILMMAKER PAUL VERHOEVEN’ (20190403 – https://michelvanderburg.com/2019/04/03/jesus-the-man-filmmaker-paul-verhoeven/ ).

EN – Filmmaker Paul Verhoeven’s project Jesus of Nazareth – a realistic portrait. Discussion with Paul Verhoeven March 31 , 2009 in dutch on his fascination for ‘Jesus the man’, his study in the Jesus Seminar, and the making of his book (based on interviews with Rob van Scheers) published a few months earlier – 10 years ago.
Paul Verhoeven was invited by the Institute of Religious Studies at the Leiden University on March 31 , 2009 in the Lokhorst church in Leiden , Holland.
A 45 min report of the 2 hours discussion – filmed with a Sony T500 compact camera.
Note : Part of this film (ca 17 minutes) was reported April 3, 2019 in the preliminary post JESUS THE MAN – FILMMAKER PAUL VERHOEVEN (20190403 – https://michelvanderburg.com/2019/04/03/jesus-the-man-filmmaker-paul-verhoeven/ ).

Credits
PAUL VERHOEVEN : JESUS THE MAN
March 31 , 2009 – Discussion Paul Verhoeven with Leiden University Prof Jürgen Zangenberg, dr Wim Hofstee, dr Peter Verstraten, students and public in Lokhorstkerk church , Leiden , Holland.
Film ‘PAUL VERHOEVEN : JESUS THE MAN’ (20191117) by Michel van der Burg – michelvanderburg.com | miracles.media