Front page Acta Diabetologica • Download Abstract Below
Abstract published in Acta Diabetologica 1998 of Iodixanol-UWS Human Islet Purification in 1998 at the Diabetes Research Institute in Miami. Abstracts book of the 18th Workshop of the AIDSPIT Study Group in Igls, Austria, Jan 1999.
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Abstract). Acta Diabetol 1998; 35: 247. View / Download abstract (file 1MEMO_20251102_1.pdf) below .
Abstract added in full post : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • 20241202 • URL michelvanderburg.com/2024/12/02 •
Citation info : Iodixanol-UWS Human Islet Purification 1998 Abstract • Michel van der Burg • Miracles.Media • @1MEMO 20251102 • URL michelvanderburg.com/2025/11/02/
Screenshot Post Saturday, July 12, 2008 at site michelvanderburg.com , hosted at Apple MobileMe.
Djembe video attracks a lot of visitors
Saturday, July 12, 2008
Tonight YouTube ranked “Channel012” as the “# 67 – Most viewed – Directors – Netherlands” channel. Thanks to the many visitors watching the Djembe-Fote video over the last day — great! Watch the video at this blog’s entry on July 9. Or just click here.
Djembe-Fote • @1MEMO 20080710
The video (10 min) was uploaded 2 days earlier , Jul 10, 2008 . “Djembé-Foté” performance on May 16, 2008 at the “Open Podium” in the “Agnietenhof Theatre” in Tiel (Netherlands). URL https://youtu.be/FGub8rXtg2U
The actual performance was 17 min. However 10 min was the max for YouTube uploads in 2008.
May 16, 2017 I uploaded de full film :
Djembé Foté ~ Full Film , Holland 2008 • @1MEMO 20170516 •
Citation info : YouTube # 67 Most Viewed Directors Netherlands • Michel van der Burg • Miracles•Media • @1MEMO 20251022 • TakeNode b2837064-e877-443b-a12a-e7ffd7ea2d77
#24 Most Viewed Today on YouTube • TRANSPORT XX – installation Brussels
My ‘Channel 012’ ranked the #24 Most Viewed Today YouTube channel in the Netherlands on the first day after uploading the captivating video Transport XX . The next day again it reached #23 Most Viewed Today.
Channel012 ranking was #68 in Most Viewed (This Week) , a week after uploading Transport XX.
Google purchased YouTube late 2006. I started late 2007, with a first upload. Between ~ 2007-2011 channel pages were equipped with view counters, bulletin boards, and were awarded badges for various rank-based achievements, such as ” “#68 – Most Viewed (This Week)”. The term “gurus” on YouTube referred to a specific category of content creators who focused on education. This category helped establish YouTube as a platform for influencers. You could change your account type to eg. Director, Comedian, Musician.
The film was uploaded close to midnight April 19th, 2009, registered by YouTube as April 20th, 2009. This short documentary is a video impression (2009) of the confrontation of passers-by with the TRANSPORT XX installation in Brussels, that presented photographic portraits of 1,200 of the 1,631 Jewish prisoners deported with the 20th train convoy to Auschwitz , the night of April 19th in 1943. Half an hour after the departure of this transport XX three young Belgians from Brussels, Youra Livschitz, Jean Franklemon and Robert Maistriau stopped the train between Boortmeerbeek and Haacht, opened one of the cars and liberated 17 prisoners. Later before the train reaches the German border over 200 other prisoners decide to attempt to escape and also jump out of the cars. This was the only documented attack on a death train during the Holocaust.
A 1minute edition of Transport XX premiered in the Paradiso theater in Amsterdam Nov. 28, 2010 – in The One Minutes – Where history starts …film festival . Here the registration : Transport XX in Paradiso | The One Minutes Collection | 20200607 | URL https://youtu.be/KuyQzLXQo3g
A behind the scenes film of the making of this film was published last year : Flow | Miracles Moment #1 | 20240513 | URL https://youtu.be/iwQQLvGtUm0
Citation info : Youtube Creator Top 25 Award • Transport XX • Michel van der Burg • Miracles•Media • 20251021 • TakeNode 39bdf260-095a-4749-acd7-e18adc9108c5 / 9f425f47-55b0-4637-9c1c-019879110895
Big surprise today , to encounter this nice news article by journalist Rob van Dijk reporting on our pancreatic islet transplantation breakthrough in the Leiden University Hospital (now LUMC) in the Amsterdam based Dutch national newspaper Parool , Saturday 6 January 1990 (1).
I was searching actually for a better copy of a different news article I have archived on my clinical islet transplantation work (Newspaper Volkskrant 10 April 1999) in the Delpher archive of the National Library of the Netherlands , scrolling the search results, and noticed this Parool article – I didn’t know it existed 😉
Journalist Rob van Dijk did not interview me for the Parool, so I assume his source was the interview I gave for publication in Cicero, the biweekly LUMC publication, and the Dutch newspaper ‘Leidsch Dagblad’, and posted a year ago (2).
The articles reports (my translation from dutch*) :
Transplantation
Islet transplantation has been possible for a few years now, but it doesn’t (yet?) work in humans. The technique itself isn’t that complicated: as many islets of Langerhans as possible are extracted from a pancreas, injected into the diabetic patient’s liver or spleen, and thus, start producing insulin. However, that “thus” is still a bit tricky… …
The major problems lie in the fact that the yield of properly functioning islets is too small (at least 30 percent of the islets must be obtained undamaged to achieve sufficient insulin production after a transplant) and that the suspensions are too impure. Nevertheless, progress is being made.
The best news comes from Leiden University Hospital. There, biologist M. van der Burg, MSc has developed a method to isolate a very high and pure yield of islets from a dog’s pancreas. Van der Burg succeeded in isolating 40 percent of the vital islets from a dog’s pancreas. Through special processing, he was able to completely purify these cell clumps of 90 percent of excess and complicating pancreatic tissue. These islets were injected into the spleen of the same dog, where they produced sufficient insulin. This initial experiment has since been repeated in Leiden with several dogs with the same success. However, major challenges remain, not the least of which is the rejection problem. The Leiden experiments used the dog’s own tissue. The big question remains how the human immune system reacts when islets from a donor are injected.
*NL (dutch) quote :
Transplantatie
Sinds een paar jaar is eilandjestransplantatie wel mogelijk, alleen, bij de mens werkt het (nog?) niet. De techniek is op zichzelf niet zo ingewikkeld: men haalt zoveel mogelijk eilandjes van Langerhans uit een pancreas, spuit die bij de suikerzieke in lever of milt in en dan gaan ze dus insuline maken. Met dat ‘dus’ ligt het echter nog moeilijk… …
De grote problemen schuilen hem erin dat de oogst aan goed werkende eilandjes te gering is (men moet ten minste 30 procent van de eilandjes onbeschadigd te pakken krijgen om na een transplantatie voldoende insulineproduktie te verkrijgen) en dat de suspensies te onzuiver zijn. Toch zit er schot in.
Het beste nieuws komt uit het academisch ziekenhuis te Leiden. Daar heeft de bioloog drs. M. van der Burg een methode ontwikkeld om uit een hondepancreas een zeer hoge en zuivere oogst aan eilandjes te isoleren. Van der Burg slaagde erin uit de alvleesklier van een hond 40 procent van de vitale eilandjes te isoleren, door een speciale bewerking kon hij deze celklompjes voor 90 procent absoluut zuiveren van overbodig en complicerend pancreasweefsel. Deze eilandjes werden ingespoten in de milt van dezelfde hond en produceren daar voldoende insuline. Dit eerste experiment is in Leiden intussen met meerdere honden met hetzelfde succes herhaald. Maar er zijn nog grote problemen op te lossen en bepaald niet het geringste daarvan is dat van de afstoting. Bij de Leidse experimenten werd gebruik gemaakt van eigen weefsel van de hond. De grote vraag blijft hoe het immuunsysteem van de mens reageert als er eilandjes van een donor worden ingespoten.
Citation info : Islet Transplantation LUMC News Surprise • Michel van der Burg • Miracles.Media • @1MEMO 20251020 • URL michelvanderburg.com/2025/10/20/
Jun 21, 2007, I started pioneering at YouTube, creating Channel012. The first YouTube video ‘Fire Breather’ was shot at a 4:3 aspect ratio (640×480 pixels) and produced at a 16:9 aspect ratio with black bars for my first YouTube upload on Dec 30, 2007.
Unlisted ‘Fire Breather • 2nd Edition’
Next a 2nd edition of ‘Fire Breather’ was produced at the original 4:3 aspect ratio, and uploaded the next month, Jan 18, 2008. YouTube displayed that 2nd edition at a higher quality. Sometime later though I decided to change visibility of that 2nd edition to ‘unlisted’ in order to preserve and favor the 1st edition views count.
Lost Forgotten Film
More recently that forgotten unlisted 2nd edition video appeared to be lost somehow…until I learned today that YouTube had changed visibility in 2021 of all unlisted videos uploaded before 2017 to ‘private’ automatically for security reasons with a worldwide system change.
Today I changed visibility at YouTube of that 2nd edition (video shown above) to public.
Summer 1997, Onno Terpstra, the head of our Surgery Department, asked me to explore our options for starting clinical islet isolation and transplantation in our center – the Leiden University Medical Center (LUMC), and prepare a roadmap.
September 1997, at the IPITA congress in Milan (1) , I learned from Camillo Ricordi, the scientific director of the Diabetes Research Institute (DRI) in Miami – where many guest collaborators work – that visiting the DRI some weeks might be a good option to gain sufficient first work experience in his clinical islet transplantation group.
December 1997, with a ‘Roadmap’, I got the green light to start the operationalization of clinical islet transplantation in the LUMC, first with a working visit to Miami to study the state of the art ‘Ricordi’ method of human islet isolation, and then start the human islet isolation work in our Surgery lab in Leiden.
In 1990 I had already gained some initial research experience in our Leiden Islet Laboratory with a small number of isolations of islets from human donor organs, using our innovative method of density gradient purification based on the UW solution (2).
During the first three months of 1998 I worked intensively with my student colleague Josephine Rijkelijkhuizen so that she would be able to continue our xenotransplantation research project as independently as possible in our Surgery research team.
April 1998, after participating in the 1st Workshop on Clinical Islet Transplantation in New Orleans (3) – the ideal introduction – a fruitful working visit to the DRI in Miami followed (4,5), with intensive collaboration with the DRI team on, among other things, six human islet isolations, and also the simultaneously (parallel) conducted research into the purification of the human islets in our Iodixanol gradient in UW-Solution (6).
Here a slideshow illustrating this team up with the DRI team in Miami , working on human islet isolation and purification in the innovative Iodixanol-UWS gradient, April-May 1998.
Notes
1) Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250128 • TakeNode • URL michelvanderburg.com/2025/01/28/
2) Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/01/14/
3) Invited participant 1st Workshop on Clinical Islet Transplantation, New Orleans (LA, USA) April 17–19, 1998
4) Working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA)
Citation info : Human Islet Isolation • DRI Miami 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250204 • TakeNode 86e4a319-a769-44d5-8fa5-de2ca90ab63c • URL michelvanderburg.com/2025/02/04/
Optiprep For Human Islet Purification • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250203 • TakeNode bad757b8-674c-4371-938c-ac73287bc457
Poster (#57) presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland (1,2)
Outcome of the first series of human islet purifications with the novel Iodixanol -UWS density gradient — developed in the Leiden Islet Laboratory of the LUMC — performed during my working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA).
Notes
1) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 .
2) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification (Abstract). Cell Transplant 1999; 8: 184. Download abstract below 1MEMO_20250202_3
Citation info : Optiprep For Human Islet Purification • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250203 • URL michelvanderburg.com/2025/02/03/
