Islet Isolation Assessment • 4th IPITA Amsterdam 1993 • @1MEMO 20260201

4th IPITA , Amsterdam, 1993 • Michel van der Burg • Miracles•Media • 1MEMO_20260201_11 • TakeNode 1c776460-e3a6-46df-8b8b-f43f30517021

Slideshow (handout slides, partially remastered) of my talk titled ‘Assessment of islet isolation efficacy in dogs’ presented at the 4th International Congress on Pancreas and Islet Transplantation (4th IPITA), Amsterdam (The Netherlands) June 27–30, 1993 • Michel van der Burg • Miracles•Media • 1MEMO_20260201_1 • TakeNode 628b273c-b8e1-4e23-8f8b-1550c7960cb3 • PDF available below.

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Text of talk ‘Assessment of islet isolation efficacy in dogs’ presented at the 4th International Congress on Pancreas and Islet Transplantation (4th IPITA), Amsterdam (The Netherlands) June 27–30, 1993 • Michel van der Burg • Miracles•Media • 1MEMO_20260201_12 • TakeNode 9bc525ba-dfdb-4d92-8af4-bdd21ee1faaf • PDF available below.

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The proceedings paper delivered at the 4th IPITA is included below in the Notes , as well as references of the related full papers and related chapters of PhD thesis.

This IPITA meeting in Amsterdam was also a good opportunity to catch up with colleague Nick London (see photo) from the Leicester (UK) group discussing our Leiden and Leicester islet isolation work using the University of Wisconsin organ preservation solution (UWS). The Leicester group were one of the first groups to examine and adopt our novel method of islet isolation in UWS.

Several groups reported at this 4th IPITA symposium on the effects of using (modified) UW solution (UWS) during islet isolation : Leicester (pig, human, islets), Oxford (rat, rabbit islets), Minneapolis (human islets), Paris (pig islets), and Giessen (clinical human allo islet transplantation).

Michel van der Burg (Leiden) and Nick London (Leicester) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Matthias Ohimer , Michel van der Burg • Miracles•Media • @1MEMO_20260201_3 • TakeNode c4451ac4-84fc-4068-823a-b475dc0bb35e • #islets #LUMC #transplant #IPITA #diabetes #Amsterdam #Leicester #Leiden

During the 4th IPITA, I discussed in detail the rationale of our UWS work, the important components of UWS, and basal solution for islet purification (Percoll-UWS) with Yoko Mullen (in particular) and her co-worker Charles Brunicardi (see photo) of the UCLA Islet Transplant Team (LA, USA)…contributing to the development of Yoko’s Los Angeles preservation solution #1 (LAP-1) patented in 1997 (see Notes, Patent US5679565A).

Michel van der Burg (Leiden), Charles Brunicardi (LA), and (? Norm Kneteman, Edmonton) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Matthias Ohimer , Michel van der Burg • Miracles•Media • @1MEMO_20260201_4 • TakeNode 93930fd4-ee21-4b18-90ca-83e1731102e1 • #islets #LUMC #transplant #IPITA #diabetes #Amsterdam #Leiden #LosAngeles #LA #Edmonton

The official founding meeting of the IPITA was actually at this 4th International Congress on Pancreas and Islet Transplantation — June 27–30, 1993 in Amsterdam (The Netherlands) hosted by Dr. Reinout van Schilfgaarde (see photo).

Reinout van Schilfgaarde (Groningen) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Matthias Ohimer , Michel van der Burg • Miracles•Media • @1MEMO_20260201_5 • TakeNode 9a713895-1dc6-462a-9621-1160ddeb2d8e • #islets #LUMC #transplant #IPITA #diabetes #Amsterdam #Leiden

Below some of the photos I took during a break at the 4th IPITA in Amsterdam, near the Rijksmuseum (corner Spiegelgracht / Tweede Weteringdwarsstraat ) , and (Jane Field) at the congress venue (RAI Amsterdam).

Garth Warnock (Edmonton), Bernhard Hering (Giessen), Nick London (Leicester) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Michel van der Burg • Miracles•Media • @1MEMO_20260201_6 • TakeNode 4a31bed0-8308-4a51-9291-06d53549ef3d • #islets #LUMC #transplant #IPITA #diabetes #Amsterdam #Leiden #Edmonton #Giessen #Leicester

Garth Warnock (Edmonton), Bernhard Hering (Giessen) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Michel van der Burg • Miracles•Media • @1MEMO_20260201_8 • TakeNode ffbe0d97-be31-4dc9-bc94-9e2b708ccb47 • #IPITA #Amsterdam #islets #LUMC #transplant #diabetes #Edmonton #Giessen #Leiden

Jane Field (Minneapolis) • 4th IPITA , Amsterdam (The Netherlands) June 27–30, 1993 • Photo by Michel van der Burg • Miracles•Media • @1MEMO_20260201_9 • TakeNode d1921eb6-2c38-49c2-9693-d288d940268d • #IPITA #Amsterdam #islets #LUMC #transplant #diabetes #Minneapolis #Leiden

Notes

Oral presentation 4th IPITA : Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Prins FA, Bruijn JA, Gooszen HG. Assessment of islet isolation efficacy in dogs. 4th International Congress on Pancreas and Islet Transplantation, Amsterdam (The Netherlands) June 27–30, 1993.

Oral presentation (selection of slides Assessment of islet isolation efficacy in dogs). Van der Burg MPM. Workshop: Guidelines for the standardized assessment of retrieval and transplantation of islets. 4th International Congress on Pancreas and Islet Transplantation, Amsterdam (The Netherlands) June 27–30, 1993.

Proceedings paper 4th IPITA : Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Prins FA, Bruijn JA, Gooszen HG. Morphometry of native and isolated islets: a new approach to isolation assessment. Transplant Proc. 1994 Apr;26(2):632-3. PMID: 7513459 . PDF available below

PMID_7513459 Download

This slideshow had been presented earlier that year with a different title at the 12th Workshop of the AIDSPIT Study Group in Igls :
Oral presentation 12th AIDSPIT : Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Bruijn JA, Prins FA, Gooszen HG. Quantification of islet isolation efficiency in dogs. 12th Workshop of the AIDSPIT Study Group of the European Association for the Study of Diabetes, Igls (Austria) January 24–26, 1993.

Title Slide (remastered) 12th AIDSPIT, Igls (Austria), 1993 • Michel van der Burg • Miracles•Media • 1MEMO_20260201_2 • TakeNode 2cfe23eb-cbb0-4942-afcb-56755ebcb467

Abstract 12th AIDSPIT : Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Bruijn JA, Prins FA, Gooszen HG. Quantification of islet isolation efficiency in dogs (Abstract). Horm Metab Res 1993; 25: 52. 1MEMO_20260201_10 PDF available below.

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Corresponding Full Papers (Thesis chapters)

Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Bruijn JA, Gooszen HG. Impact of donor-related variables on islet isolation outcome in dogs. Diabetologia. 1994 Jan;37(1):111-4. doi: 10.1007/BF00428786. PMID: 8150223 . Article PDF Free download.

Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Prins FA, Bruijn JA, Gooszen HG. Assessment of islet isolation efficacy in dogs. Cell Transplant. 1994 Jan-Feb;3(1):91-101. doi: 10.1177/096368979400300113. PMID: 8162296

Chapters (2,3) Doctoral Thesis (corresponding with full papers) : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Dec 4th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 . PDF free download available via link.

Patent US5679565

Method of Preserving Pancreatic Islets , Mullen et al. United States Patent US5679565 . Download PDF https://patentimages.storage.googleapis.com/80/60/f6/744731dd250c48/US5679565.pdf

Online history of patent :
US5679565A – Method of preserving pancreatic islets – Google Patents URL https://patents.google.com/patent/US5679565A

Portfolio

Online Portfolio – Science : https://michelvanderburg.com/category/science/

#islet #IPITA #AIDPIT #Amsterdam #Igls #Innsbruck #UWS #density #purification #history #AIDSPIT #Leiden #Leicester #Netherlands #Austria #Langerhans #isolation #transplantation #pancreas #diabetes #insulin #donor #assessment #michelvanderburg #symposium #workshop #science #EPITAsymposium #EASD #ESOT #Edmonton #Giessen #Minneapolis

Citation info : Islet Isolation Assessment • 4th IPITA Amsterdam 1993 • Michel van der Burg • Miracles•Media • @1MEMO 20260201 • URL https://michelvanderburg.com/2026/02/01

Glycemic control mechanisms after canine islet autografting • IPITA 1995• @1MEMO 20251204

Glycemic control mechanisms after canine islet autografting • IPITA 1995 • Michel van der Burg • Miracles.Media • @1MEMO 20251204_1 • Talk, Tuesday June 20, 1995 at 10:30, 5th IPITA, Eden Roc Resort Hotel, Miami Beach, FL (USA) • Digitally remastered by author, publisher, Michel van der Burg • TakeNode 5227c200-e029-4406-b344-15350fe3b93d • #GLP1 #GIP #diabetes #islets #transplant

Pioneer work on GLP-1 and GIP incretin hormones

Slideshow (digitally remastered) of my talk Tuesday June 20, 1995 at 10:30, at the 5th International Congress on Pancreas and Islet Transplantation (IPITA), in Miami Beach, FL (USA). My first talk at a world congress presenting the outcome of all our detailed metabolic studies in our preclinical model of canine islet autotransplantation , including our pioneer work on the incretin effects of the gut hormones GLP-1 (glucagon-like peptide-1) and GIP (Glucose-dependent Insulinotropic Polypeptide) — now, in 2025, well-known from the drugs that mimic the action of these natural hormones, to treat type 2 diabetes and obesity, by stimulating insulin release, improving blood sugar control, and reducing appetite.

May 1992 we were the first to show direct potentiation of the insulin response from isolated islets by (near-)physiological doses of both the gut hormones GLP-1 and GIP during perifusion (in vitro) of canine islets at 7.5-10 mM glucose levels (1,2), and January 1994 we next confirmed the incretin effect of a (near-)physiological dose of GLP-1 after canine islet transplantation , in a poster presented at the 13th Workshop of the AIDSPIT (aka AIDPIT) Study Group , Igls, Austria, January 23–25, 1994 (3).

Background of the work was posted earlier this year (4) and the work was published Nov 1994 in my PhD thesis Pancreatic islet transplantation , available online (5).

Welcome Reception 5th IPITA on Sunday 18 June 1995, at the congress venue , the Eden Roc Resort Hotel, in Miami Beach, FL (USA) • Michel van der Burg • Miracles.Media • @1MEMO 20251204_2 • TakeNode 8eb04df1-8280-4de3-a206-4b63413c3025

June 1995, 5th IPITA Talk

Tuesday June 20, 1995 at 10:30, at the 5th International Congress on Pancreas and Islet Transplantation (IPITA), in Miami Beach, FL (USA), was my first talk at a world congress presenting the outcome of all our detailed metabolic studies in the canine islet transplants , including the incretin effect of GLP-1, and also our findings with isolated canine islets during perifusion, in vitro, of the direct insulinotropic effects of the gut hormones GIP and GLP-1 (6) . That slideshow presentation (digitally remastered*) is posted here (1MEMO 20251204_1).

The proceedings paper delivered at the 5th IPITA is included in the Notes (7) , as well as the referenced previous papers (8,9)

Notes

1. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Functie na intralienale transplantatie van eilandjes van Langerhans in de hond ( Function after intrasplenic transplantation of islets of Langerhans in dogs ). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992. In : Gut factors controlling pancreatic islets • 20240910 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/09/10/gut-factors-controlling-pancreatic-islets-20240910/

2. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

3. Metabolic control after canine islet transplantation. M.P.M. van der Burg, P.T.R. van Suylichem, O.R. Guicherit, J.H.M. van Deijnen, M. Frölich, and H.G. Gooszen (Universities of Leiden & Groningen, The Netherlands). Poster presented at the 13th Workshop of the AIDSPIT Study Group , Igls, Austria, January 23–25, 1994 • In : GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • Michel van der Burg • Miracles.Media • @1MEMO 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • URL https://michelvanderburg.com/2025/11/14

4. Background of this work was posted in Dutch Islets Transplant Team • @1MEMO 20250125 • URL https://michelvanderburg.com/2025/01/25/dutch-islets-transplant-team-1memo-20250125/

5. Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Dec 4th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .

6. Oral presentation : Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. 5th International Congress on Pancreas and Islet Transplantation (IPITA), Miami Beach (USA) June 20, 1995.

7. 5th IPITA Proceedings publication : Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. Transplant Proc. 1995 Dec;27(6):3187-8. PMID: 8539903. PDF available below PMID_8539903

PMID_8539903 Download

Referenced previous publications :

8. Van der Burg MPM, Gooszen HG. The metabolic efficiency of islet grafts: an overview. In: Islet transplantation — current status of clinical application and experimental results. Hesse UJ , Pichlmaier H, Eds., Lengerich Germany, Wolfgang Pabst Verlag, 1992, p.93–99. Pabst hash code 0383b3abad3ae774b146ea0425c2227a PDF available below 1MEMO_20251204_4

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9. Van der Burg MPM, Guicherit OR, Scherft JP, Lemkes HHPJ, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors (Abstract). Neth J Med 1992; 41: A25. PDF available below 1MEMO_20251204_3

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Other previous publications :

10. Van der Burg MPM, Guicherit OR, Van Suylichem PTR, Frölich M, Van Deijnen JHM, Gooszen HG. Importance of the entero-insular axis for islet graft function (Abstract). Cell Transplant 1994; 3: 257. Abstract – Second International Congress of the Cell Transplant Society May 1–4, 1994 Hyatt Regency Hotel • Minneapolis, Minnesota . https://doi.org/10.1177/096368979400300302 (Abstracts published , posters not presented). PDF available below 10.1177_096368979400300302

10.1177_096368979400300302 Download

11. Chapter 8, Doctoral Thesis, Leiden University, November 1994 : Metabolic control after intrasplenic islet autotransplantation in dogs: ß-cell secretory capacity, insulin action, and the enteroinsular axis. Michael P.M. van der Burg, Paul T.R. van Suylichem, Onna R. Guicherit, Marijke Frölich, Herman H.P.J. Lemkes, and Hein G. Gooszen. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 8). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .
Direct link to file Chapter 8 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486615/view .

12. Doctoral Thesis, Leiden University, November 1994 (Chapter 6) : lnsulinotropic properties of cholecystokinin, gastric inhibitory polypeptide, and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Michael P.M. van der Burg, Onna R. Guicherit, Marijke Frölich and Hein G. Gooszen. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 6). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .
Direct link to file Chapter 6 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486613/view

Full papers

13. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

14. Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glucoregulation after canine islet transplantation: contribution of insulin secretory capacity, insulin action, and the entero-insular axis. Cell Transplant. 1997 Sep-Oct;6(5):497-503. doi: 10.1177/096368979700600509. PMID: 9331501. PDF link https://pure.rug.nl/ws/portalfiles/portal/58359942/096368979700600509.pdf

PDF (CC-BY-NC) from publisher via ResearchGate – https://www.researchgate.net/publication/317805208_Glucoregulation_after_Canine_Islet_Transplantation_Contribution_of_Insulin_Secretory_Capacity_Insulin_Action_and_the_Entero-Insular_Axis

* 1MEMO 20251204_1 . Digitally remastered presentation, using the images from the original slides handout, and the handout of the text spoken during the original presentation , now read using my personal synthesized voice.

#GLP1 #GIP #diabetes #islets #transplant

Updates

Update 20260203 – added ResearchGate link ref 14

Citation info : Glycemic control mechanisms after canine islet autografting • IPITA 1995 • Michel van der Burg • Miracles.Media • @1MEMO 20251204 • URL https://michelvanderburg.com/2025/12/04

Islet Transplantation LUMC News Surprise • @1MEMO 20251020

Diabetic (dutch ‘Suikerzieke) … Parool, 6 Jan 1990 • @1MEMO 20251020

Big surprise today , to encounter this nice news article by journalist Rob van Dijk reporting on our pancreatic islet transplantation breakthrough in the Leiden University Hospital (now LUMC) in the Amsterdam based Dutch national newspaper Parool , Saturday 6 January 1990 (1).

I was searching actually for a better copy of a different news article I have archived on my clinical islet transplantation work (Newspaper Volkskrant 10 April 1999) in the Delpher archive of the National Library of the Netherlands , scrolling the search results, and noticed this Parool article – I didn’t know it existed 😉

Journalist Rob van Dijk did not interview me for the Parool, so I assume his source was the interview I gave for publication in Cicero, the biweekly LUMC publication, and the Dutch newspaper ‘Leidsch Dagblad’, and posted a year ago (2).

The articles reports (my translation from dutch*) :

Transplantation

Islet transplantation has been possible for a few years now, but it doesn’t (yet?) work in humans. The technique itself isn’t that complicated: as many islets of Langerhans as possible are extracted from a pancreas, injected into the diabetic patient’s liver or spleen, and thus, start producing insulin. However, that “thus” is still a bit tricky… …
The major problems lie in the fact that the yield of properly functioning islets is too small (at least 30 percent of the islets must be obtained undamaged to achieve sufficient insulin production after a transplant) and that the suspensions are too impure. Nevertheless, progress is being made.
The best news comes from Leiden University Hospital. There, biologist M. van der Burg, MSc has developed a method to isolate a very high and pure yield of islets from a dog’s pancreas. Van der Burg succeeded in isolating 40 percent of the vital islets from a dog’s pancreas. Through special processing, he was able to completely purify these cell clumps of 90 percent of excess and complicating pancreatic tissue. These islets were injected into the spleen of the same dog, where they produced sufficient insulin. This initial experiment has since been repeated in Leiden with several dogs with the same success. However, major challenges remain, not the least of which is the rejection problem. The Leiden experiments used the dog’s own tissue. The big question remains how the human immune system reacts when islets from a donor are injected.

*NL (dutch) quote :

Transplantatie

Sinds een paar jaar is eilandjestransplantatie wel mogelijk, alleen, bij de mens werkt het (nog?) niet. De techniek is op zichzelf niet zo ingewikkeld: men haalt zoveel mogelijk eilandjes van Langerhans uit een pancreas, spuit die bij de suikerzieke in lever of milt in en dan gaan ze dus insuline maken. Met dat ‘dus’ ligt het echter nog moeilijk… …
De grote problemen schuilen hem erin dat de oogst aan goed werkende eilandjes te gering is (men moet ten minste 30 procent van de eilandjes onbeschadigd te pakken krijgen om na een transplantatie voldoende insulineproduktie te verkrijgen) en dat de suspensies te onzuiver zijn. Toch zit er schot in.
Het beste nieuws komt uit het academisch ziekenhuis te Leiden. Daar heeft de bioloog drs. M. van der Burg een methode ontwikkeld om uit een hondepancreas een zeer hoge en zuivere oogst aan eilandjes te isoleren. Van der Burg slaagde erin uit de alvleesklier van een hond 40 procent van de vitale eilandjes te isoleren, door een speciale bewerking kon hij deze celklompjes voor 90 procent absoluut zuiveren van overbodig en complicerend pancreasweefsel. Deze eilandjes werden ingespoten in de milt van dezelfde hond en produceren daar voldoende insuline. Dit eerste experiment is in Leiden intussen met meerdere honden met hetzelfde succes herhaald. Maar er zijn nog grote problemen op te lossen en bepaald niet het geringste daarvan is dat van de afstoting. Bij de Leidse experimenten werd gebruik gemaakt van eigen weefsel van de hond. De grote vraag blijft hoe het immuunsysteem van de mens reageert als er eilandjes van een donor worden ingespoten.

Notes

1) Transplantatie. “Het Parool”. Amsterdam, 06-01-1990. Retrieved in Delpher on 20-10-2025, https://resolver.kb.nl/resolve?urn=ABCDDD:010834140:mpeg21:p017

2) Islet Transplantation Breakthrough in Leiden University Hospital • 20240830 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/08/30/islet-transplantation-breakthrough-in-leiden-university-hospital-20240830/

Citation info : Islet Transplantation LUMC News Surprise • Michel van der Burg • Miracles.Media • @1MEMO 20251020 • URL michelvanderburg.com/2025/10/20/

Transplants of UW-Isolated Islets • Transplantation Society 1990 Paper • @1MEMO 20250118

Congress of The Transplantation Society, Lobby of the Fairmont Hotel, San Francisco, California, USA, August 1990 • Michel van der Burg • Miracles.Media • @1MEMO 20250118_1 • TakeNode 4af71bca-edaa-4adb-9dce-556534844f5b

Following papers presented in 1989 demonstrating that over 90% pure islets are isolated from pancreases when using the UW organ preservation solution (1), the outcome of transplantation of highly purified UW-isolated islets was reported in a proceedings paper presented August 1990 at the XIII International Congress of The Transplantation Society in San Francisco, USA (2,3).
The Transplantation Proceedings paper, published Feb 1991 is now available online below (3).

Notes

1) Pancreatic Islet Isolation With UW Solution • A New Concept • Michel van der Burg • Miracles.Media • @1MEMO 20241224 • URL michelvanderburg.com/2024/12/24/

2) Van der Burg MPM, Guicherit OR, Ploeg RJ, Frölich M, Bruijn JA, Scherft JP, Gooszen HG.
Metabolic control after autotransplantation of highly purified canine pancreatic islets isolated in UW-solution. XIII International Congress of The Transplantation Society, San Francisco (USA) August 19–24, 1990.

3) van der Burg MPM, Guicherit OR, Ploeg RJ, Frölich M, Bruijn JA, Scherft JP, Gooszen HG. Metabolic control after autotransplantation of highly purified canine pancreatic islets isolated in UW solution. Transplant Proc. 1991 Feb;23(1 Pt 1):785-6. PMID: 1990691. PDF available below

PMID_1990691🔗Download

Citation info : Transplants of UW-Isolated Islets • Transplantation Society 1990 Paper • Michel van der Burg • Miracles.Media • @1MEMO 20250118 • URL michelvanderburg.com/2025/01/18/

Diabetes Discoveries Display • @1MEMO 20250117

Montage of 100 years diabetes discoveries in educational wall display (created in 1998) from the Joslin Diabetes Center and Roche Diagnostics.

Music : Someday, Maybe by Maya Belsitzman & Matan Ephrat | Artlist

Citation info : Diabetes Discoveries Display • Michel van der Burg • Miracles.Media • @1MEMO 20250117 • TakeNode 00ff4392-ea87-40fb-90a2-ada2cbb77efb • URL michelvanderburg.com/2025/01/17/

Pancreatic Islets UWS Series 1989 • Kodak PhotoCD • @1MEMO 20250103

4th ESOT Slide #19 • Pancreatic Islets UWS Series 1989 • Kodak PhotoCD • Michel van der Burg • Miracles.Media • 20250103_1 • TakeNode eeb55729-c02b-4f73-ab0e-9f71b65bf85e

Slide #19 of talk by Michel van der Burg at the 4th ESOT congress in Barcelona (Spain) Nov 1989 on the paper ‘Pancreatic Islet Isolation With UW Solution • A New Concept’ (1,2). Details of this talk were posted recently (2) with a scan of a paper print of slide #19 – slighly cropped with printing.
Here the full image of the original slide, scanned by Kodak PhotoCD in 1994, with minor color correction added (20250103_1) in this post.

This ‘Slide #19’ shows the final dithizone stained sample of the UW-isolated islets after dextran density gradient purification — a micrograph made April 18, 1989 , the second islet isolation experiment performed using the UW-Solution as the isolation solution.

In the lab journal of the first isolation experiment with UW-Solution, Wednesday April 12th 1989, I noted on the final purified islet preparation : ‘handpicking for encapsulation was not necessary: preparation more than 90% pure ( translated from dutch : “handpicken voor inkapseling was niet nodig: preparaat meer dan 90% zuiver”)’.

‘Handpicking’ of islets had been done at the start of our islet lab in 1985-1987 for counting rodent islets in cell culture dishes, and monitoring islet insulin secretion in tubes (3). Soon, ‘handpicking’ was no longer needed , when monitoring the insulin secretion of isolated islets in perifusion chambers (4,5). However, the handpicking method was used again in a pilot study on encapsulation of islets in 1989 by medical student Joost Clemens (6). Joost had just started on this research project, and was assisting me with islet ‘counting’ (yield and purity assessment) in the ‘duplo’ samples from this first series of UWS isolations.

Handpicking Pancreatic Islets • Michel van der Burg • Miracles.Media • 20250103_2 • TakeNode 7988ce39-6777-4db3-82c5-8191acdb13d6

Macro photography (Photo paper print, September 1987, AVC, Leiden University) of the aspiration of isolated canine pancreatic islets, in a suspension still contaminated with exocrine pancreatic fragments, at the tip of a hand blown glass handpicking pipette (custom made at the Leiden University) collected and transferred into tubes for monitoring insulin secretion the first year of pilot work in the Leiden islet laboratory (4).

Notes

1) Pancreatic Islet Isolation With UW Solution • A New Concept • Michel van der Burg • Miracles.Media • @1MEMO 20241224 • URL https://michelvanderburg.com/2024/12/24/pancreatic-islet-isolation-with-uw-solution-a-new-concept-20241224/

2) Pancreatic Islet Isolation in UW Solution & Transplantation • ESOT 1989 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20241229 • URL michelvanderburg.com/2024/12/29/

3) Lucky #1 Islet • 20240811 • Michel van der Burg • michelvanderburg•com

4) Cradle of Islet Transplants • 20240829 • Michel van der Burg • michelvanderburg•com

5) Monitoring pancreatic islets in perifusion • 20240911 | Michel van der Burg | michelvanderburg•com

6) Joost A.M. Clemens (MSc student in Medicine) at the Department of Biomaterials (Prof. dr K. de Groot) and Department of Surgery of the Leiden University (Michel van der Burg , Hein Gooszen). Pilot study of the encapsulation of dog islets using the alginate polylysine and poly-HEMA-phospate method (1989).
At that time , both the Biomaterials department and the Islet Lab of the Surgery department were housed in the building of the Cell Biology department of the Leiden University Hospital (AZL, now LUMC). Below a portrait snapshot I took of Klaas de Groot at a Garden party organized in 1989 by the Department of Cell Biology, Leiden University.

Klaas de Groot, Biomaterials Department of the Leiden University in 1989. Photo by Michel van der Burg • Miracles.Media • 20250103_3 • TakeNode c25a87b7-09a4-49ce-8c42-14142da1e066

Citation info : Pancreatic Islets UWS Series 1989 • Kodak PhotoCD • Michel van der Burg • Miracles.Media • @1MEMO 20250103 • URL michelvanderburg.com/2025/01/03/

Pancreatic Islet Isolation in UW Solution & Transplantation • ESOT 1989 Talk • @1MEMO 20241229

Oral Presentation Slide 19 ~ Pure Islets Isolated in UW Solution • Miracles.Media • 20241229_1 • TakeNode ce420e5a-1df7-4e4f-bcb6-6831434ffac7

In November 1989, I had my first chance for a presentation at a major international conference, the 4th ESOT meeting in Barcelona, of a paper centered on the rationale for employing the University of Wisconsin (UW) organ preservation solution in pancreatic islet isolation.

Venue 4th Congress of the European Society for Organ Transplantation, Barcelona (Spain) November 1–4, 1989 • Michel van der Burg • Miracles.Media • 20241224 • TakeNode 103c4c3c-5d36-4111-a5f8-f60e81c5db4d

This innovative islet isolation work with the UW-Solution had been accepted as a poster presentation for the 4th ESOT meeting (1).
I also took the opportunity to present this new work during a talk on previous isolation work (2,3) at this conference.

Barcelona Nov 1989 4th ESOT • Talk by Michel van der Burg • Miracles.Media • 20241229_2 • Photo : Grup Jaume Muntaner (Barcelona) • TakeNode 96957e2f-1462-48e1-aca1-4745198d9a54

‘Behind the scenes’ I had first remade my lost poster at the congress venue (1), and then I rewrote my speech in the hotel room on the older research (2) updated with the more recent work with the UW-Solution, and including the outcome of our first islet transplantations.

Barcelona 89 • Handout 4th ESOT Talk

Below a scan of that handout of the final speech, written out at full lenght in a notepad from a recent working visit in Minneapolis (4).

Barcelona 89 • Handout 4th ESOT Talk • Michel van der Burg • Miracles.Media • 20241229_3 • TakeNode f8595a82-fba5-4a89-a01f-4c37976d06d7 • PDF FILE 20241229_3 copy is available here

20241229_3 🔗Download

Handout Page 1 • 20241229_3 • Miracles.Media

Speech Excerpts

…

( finishing speech first part on our previous islet isolation methods using the conventional RPMI solution – a solution originally designed for tissue culture, not for cold storage of organs )

Slide 18

This slide shows one of the purest preparations obtained, with an estimated purity of 65% islets.
• On the average however a purity of only 30% was obtained.

Recently we adopted an entirely new approach to islet isolation.
• Since UW, the new organ cold storage solution has been shown to allow long-term cold storage of the canine pancreas;
• and, since islet isolation too is largely performed in the cold;
• we tested this preservation solution as the isolation medium, throughout the isolation procedure in another 6 dogs;
• as compared to the commonly used solutions like RPMI tissue culture medium.

Slide 19

This slide shows the final dithizone stained preparation after dextran purification of UW-isolated islets.
• UW did not affect islet yield
• however, UW markedly improved purity :
• acinar tissue is virtually absent.

…

Notes

2) Van der Burg MPM, Gooszen HG, Field MJ, Scherft JP, Terpstra JL, Van de Woude FT, Guicherit OR, Frölich M, Bruijn JA. Comparison of current islet isolation techniques in dogs. 4th Congress of the European Society for Organ Transplantation, Barcelona (Spain) November 1–4, 1989. Oral presentation. (1)

3) Van der Burg MPM, Gooszen HG, Field MJ, Scherft JP, Terpstra JL, van de Woude FJ, Guicherit OR, Frölich M, Bruijn JA. Comparison of current islet isolation techniques in dogs. Transplant Proc. 1990 Aug;22(4):2044-5. PMID: 1697121.
This paper appears to be not readily available online – a reprint copy is available at this site (1)

4) Working visit Sep – Oct 1989, joining the pancreatic islet isolation team of the pioneer transplant surgeon David Sutherland at the University of Minnesota in Minneapolis.

Citation info : Pancreatic Islet Isolation in UW Solution & Transplantation • ESOT 1989 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20241229 • URL michelvanderburg.com/2024/12/29/