Tag Archives: research

Flammable Films Bunker

Flammable Films Bunker
Former german bunker (Atlantic Wall) in the dunes along the North Sea near Scheveningen , The Hague, with special safes to store the flammable nitrate films of dutch history from the national dutch archive – historic films for safety reasons not allowed within built-up areas.
Here the Westerbork film footage also was examined in the 1990s for clues about the deportation trains and the name of the girl with the ‘working’ name Esther (by researcher Aad Wagenaar) – later to be identified as Settela.
Here a 1980 Polygoon newsreel on the restauration of this RVD archive bunker with new safes.

Source : Polygoon-Profilti (producer) / Netherlands Institute for Sound and Vision (curator).
Film edit ‘Flammable Films Bunker’ (20191216) by Michel van der Burg | Settela.com – licensed CC BY-SA 4.0

Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients

Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients.
Here a film made of my ‘powerpoint’ presentation Feb 22, 2000 – on behalf of our Surgical Department – at the Opening Symposium of the Interdivisional GMP (‘Good Manufacturing Practice’) facility of the Leiden University Medical Centre – the LUMC in Leiden, Holland.

I first started – with a background in biochemistry and philosophy – some 20 years earlier (from 1981) research at the Department of Surgery of the Leiden University Hospital (AZL) in the field of transplantation of the pancreas organ to cure diabetes.

Succesful Experiment Islet Transplantation
From 1986 my focus shifted to innovative procedures for the isolation out of the pancreas organ, of the ca 1 million islets of Langerhans – the insulin producing cell clumps with a size between ca 0.1 to 1 mm , that is 0.004 to 0,04 inch.
In 1989 we performed the first successful autologous transplantations in Holland (in collaboration with the Minneapolis Islet Lab with Jane Field and David Sutherland) – for the first time world-wide with pure islets using our novel procedure of using the organ preservation UW solution (University of Wisconsin / collaboration with dr Robert Carter , Du Pont, UK) during isolation in a pre-clinical large animal diabetes model – with long term transplant functioning with near-normal blood sugar regulation.

First series of human islet isolations , 1989-1990
Next in 1989-1990 we performed a first series of islet isolations from human donor pancreases using our novel techniques of isolation and purification in UW organ preservation solution – in association with a European Concerted Action for the Treatment of Diabetes – the Brussels headed (Daniel Pipeleers) ‘Multicenter program on the treatment of diabetes by islet cell transplantation’.

Exploring human islet work
Subsequently – after some years of exploring options for animal to human islet transplantation , using pig pancreas islets – so-called xenotransplantation – I was asked in 1997 to explore the requirements for operationalization of clinical islet transplantation in the LUMC. December 1997 our Surgical Department decided to go for it.

Establishing the human islet isolation laboratory in Leiden.
In 1998 after a month working in dr Camillo Ricordi’s Diabetes Research Institute in Miami, I started a large series of human islet isolation procedures in Leiden, and from september 1998 documenting the detailed GMP (‘Good Manufacturing Practice’) standard operating procedures, as well as ‘building’ our clinical islet isolation clean room with help from the Miami team in close cooperation with dr. Amon Wafelman (department of Clinical Pharmacy & Toxicology) managing the building of our brand new Interdivisional GMP-facility LUMC (IGFL) of six clean rooms and two chemical product laboratories – that was opened officially Feb 22, 2000 with the symposium, a film, and a tour in the GMP facility.
One year later the GMP islet facility was fully operational and summer 2001 we got a cautious green light (GMP license) for clinical transplantation of any high quality – approved – islet preparations.
Around the same time islet transplantation suddenly became very promising with ‘The Edmonton protocol’. However with the fast increase of logistic and financial demands, and reorganization between LUMC divisions it took till 2007 to finally start first transplants by an expanded islet team in the LUMC clinical islet center in Leiden.
Worldwide since 2000 several hundred people have received islet transplants that generally do not result in long term insulin independence , but do help sugar regulation in the transplant recipients.

Notes :
Links to some publications, news paper reports etc will follow later.


Post updated with more details 20191215

Presentation ‘Isolation of Islets of Langerhans for Transplantation in Type-1 Diabetic Patients’ by Michel van der Burg, Feb 22, 2000 – Opening Symposium of the Interdivisional GMP facility of the Leiden University Medical Centre, Leiden, Netherlands. Film : 20191213 Michel van der Burg | miracles.media

RIOD 1948 ~ National Institute for War Documentation in Holland

① memo 20190808 ~ RIOD 1948 ~ National Institute for War Documentation in Holland ~ In the first years after the war, the National Institute for War Documentation in Holland , known as the ‘RIOD’ – Rijksinstituut voor Oorlogsdocumentatie – started collecting original documents about the Netherlands and the Dutch East Indies during the Second World War. In addition to collecting and organising all these documents RIOD also did research, performed historical studies into the Second World War and the Holocaust. In 1946 the RIOD started the collection of the footage filmed by the Jewish camp prisoner Rudolf Werner Breslauer spring 1944 in the Westerbork transit camp.
Here in this Polygoon cinema newsreel week 3 in 1948 the RIOD’s director Loe de Jong gives the dutch cinema public a look behind the scenes of the RIOD institute.

Credit info :

RIOD 1948 ~ National Institute for War Documentation in Holland
Source 1948 Polygoon newsreel week 3, 1948 (WEEKNUMMER480-HRE0000DED7_1757240_1937680) courtesy of Polygoon Hollands Nieuws (producer) / Nederlands Instituut voor Beeld en Geluid (OpenImages, public domain).
RIOD 1948 (20190808) Michel van der Burg | Settela.com – CC BY 4.0 .