Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • 20241203

Slides oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model. The pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.

Slideshow (pdf) oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241203_2 • TakeNode 5f53ae49-6fb8-4bef-a434-ac2ce8151c2a • File 1MEMO_20241203_2

1MEMO_20241203_2 Download

Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999

Updates

Update 20260123 : Vimeo video replaced by YouTube copy

Citation info : Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • 1MEMO 20241203 • TakeNode ea1f1abd-6227-4ae6-985b-f8188310cd24 • URL michelvanderburg.com/2024/12/03/

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • 20241202

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202_1

I started an archive at Archive.org November 11, 2010 with this slideshow (pdf) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).

The presented results of this innovative islet purification method — developed over nearly 10 years (from 1989) in our Leiden islet laboratory (LUMC) — were the outcome of work I had done spring 1998 with the human islet transplantation group of Camillo Ricordi at the Diabetes Research Institute in Miami , Florida, USA.

Slideshow (pdf) Archive.org

Description

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999

Slides oral presentation Michel van der Burg, Aidspit meeting 25 Jan 1999, in Igls, Austria

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification. 18th Workshop Aidspit, Igls (Austria) Jan. 24-26, 1999

Program :
18TH WORKSHOP OF THE AIDSPIT STUDY GROUP – JANUARY 24-26, 1999 – IGLS/AUSTRIA . MONDAY, JAN 25, 1999 – KONGRESSHAUS IGLS, MAIN LECTURE HALL
SESSION 2 – ISLET ISOLATION AND TRANSPLANTATION
CHAIRMEN:K. FEDERLIN, GIESSEN, GERMANY – G. POZZA, MILAN, ITALY 9.00 – 10.30 FREE COMMUNICATIONS
9.45- Efficacy of the novel iodixanol-UWS density gradient for human islet purification.
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C; Leiden, The Netherlands and Miami, USA

File : mvdb19990118_1534_AIDSPIT.pdf | Uploaded 20101111 and replaced by the same pdf with updated metadata of the document on 20101112 (see Notes section).

Citation info (Archive file) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL https://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet • TakeNode 54cc8b66-672e-4b13-913c-449038ed0521

Video Slideshow

The slideshow video embedded below, was first posted at 1-memo.com in 2018 as Human Islet Purification Miami 1998 • @1MEMO 20181102

Notes

The slideshow pdf file is available for download at Archive.org. I advise against the other download options presented by Archive.org , which do not correctly represent the slideshow.

The file was also uploaded to Scribd at the same time – URL https://www.scribd.com/document/42197150/Efficacy-of-the-novel-iodixanol-UWS-density-gradient-for-human-islet-purification-michelvanderburg-19990125

Abstract

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Abstract). Acta Diabetol 1998; 35: 247. View or download abstract below .

1MEMO_20251102_1 Download

Updates

20251101 – Vimeo video replaced by YouTube copy

20251101 – Added PDF copy abstract – file 1MEMO_20251102_1.pdf

Citation info (this post) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL michelvanderburg.com/2024/12/02/

ADD Diabetes Group 1989, St Edmund Hall, Oxford • 20241019

St Edmund Hall, University of Oxford, July 1989 • Michel van der Burg • Miracles•Media • 20241019_1 • TakeNode af426345-4fb3-48a3-8d5e-4368c56826aa

Venue of the Seventh Annual Meeting of the Anglo–Danish–Dutch Diabetes Group*, Oxford (UK) July 5–9, 1989.

The first international meeting were our new concept was presented, of pancreatic islet isolation in the University of Wisconsin Solution (UW-Solution or UWS) a solution designed recently for preservation of a donor pancreas during cold storage prior to transplantation.

Quote :

“…A few months ago, we adopted an entirely new approach to islet isolation.
Recently a new cold storage organ preservation solution was developed at the University of Wisconsin, the UW solution, allowing long term, 24 to 72 h, cold storage of the canine pancreas.
Since islet isolation is largely performed in the cold, we tested this preservation solution as the isolation medium throughout the isolation procedure. This slide shows that using the UW solution, purity has increased to over 90%…”

In addition our first successful pre-clinical transplant, we had performed the last month, was reported as breaking news at the end of the talk.

Quote :

“Three weeks ago we did our first autotransplantation of isolated islets from the whole gland by retrograde infusion into the spleen. The dog is doing fine: fasting glucose amounts to 5-6 mM and postprandial glucose between 6-8 mM ; postprandial insulin increases 6-7 fold. Thank you.”

St Edmund Hall, Oxford, 1989, 7th Meeting ADDD Group • Michel van der Burg • Miracles•Media • 20241019_2 • TakeNode ca40adf0-3b86-4ada-93a8-126c6768d420

Notes

Seventh Annual Meeting Anglo Danish Dutch Diabetes Group • Michel van der Burg • Miracles•Media • 20241019_3 • TakeNode bffdea47-9464-4f7b-8299-ec299d41ca4d

* Group supported through the Nordisk UK Professional Programme.

Van der Burg MPM, Gooszen HG, Scherft JP, Terpstra JL, Frölich M, Guicherit OR, Lemkes HHPJ, Bruijn JA. The isolation of canine pancreatic islets for transplantation. Seventh Annual Meeting Anglo Danish Dutch Diabetes Group, Oxford (UK) July 5–9, 1989.

Van der Burg MPM, Gooszen HG, Guicherit OR, Jansen JBMJ, Frölich M, Lamers CBHW. The mechanism of pancreatic duct obliteration induced hyperglycemia. Seventh Annual Meeting Anglo Danish Dutch Diabetes Group, Oxford (UK) July 5–9, 1989.

Citation info : ADD Diabetes Group 1989, St Edmund Hall, Oxford • Michel van der Burg • Miracles•Media • 20241019 • URL michelvanderburg•com

Powerbook 160 • 20240904

Printing journal on ‘F4UW’ (in porcine islet isolation) …Apple Powerbook 160 , Islet Laboratory, Leiden University Hospital 1990s. Found a snippet correspondence with Nycomed worker Bjørn Henriksen on F4UW :
“Dear Bjørn…I checked my journals re the use of ficoll. I have temporarily used Ficoll-400DL from Sigma in 1996 for making Optiprep-UWS gradients and UWS as the isolation solution. The reason was that I had no more Pentastarch left from a shipment received from DuPont…Most of the experiments used WOP prepared from 1:1 optiprep and either F4UWS or 8 percent Ficoll in UWS….”

Citation info : Powerbook 160 • Michel van der Burg • Miracles.Media • 20240904 • URL michelvanderburg.com/2024/09/04/ • TakeNode 7e2d8499-a369-4055-992c-0442a17027c4

Pittsburgh 1992 Cell Transplant Society • 20240812

Poster Cell Transplant Society, Pittsburgh (USA) June 3, 1992 • 20240812_1 • michelvanderburg•com • TakeNode ca498136-3e99-4486-b641-f9f61894f19c

Updated 20250115

Congress

Pittsburgh (USA) May 31–June 3, 1992. Founding meeting, and 1st Int. Congress of the Cell Transplant Society.

Michel van der Burg, Co-chair ‘Methods’ Poster Discussion CTS , Pittsburgh June 3, 1992 • 20240812_2 • michelvanderburg•com • TakeNode 509ce973-d218-4f9c-84ef-ffa7c4fdb0d1

Poster creation

The poster was designed and produced May 1992 on a Macintosh computer using MacDraw II (with bar graphs from Cricket Graph) and printed landscape across a chain of several sheets of paper using the Imagewriter dotmatrix printer.

For this 2024 remastered poster below, the 1992 MacDraw II file was converted and edited using LibreOffice Vanilla, and next Pixelmator Pro was used for additional ‘repair’ (conversion issues with the many layers used for ‘tubes’ creation) and the insertion of the photo scans.

Poster Pittsburgh 1992 Cell Transplant Society, remastered • 20240812_3 • michelvanderburg•com • TakeNode 26159e06-9f29-4fab-a5f3-a595469b352e

Notes

Van der Burg MPM, Guicherit OR, Scherft JP, Bruijn JA, Frölich M, Gooszen HG. Islet preservation during isolation: a new concept in cell transplantation. First International Congress of the Cell Transplant Society, Pittsburgh (USA) May 31 – June 3, 1992.

Van der Burg MPM, Guicherit OR, Frölich M, Bruijn JA, Gooszen HG. Islet preservation during isolation: a new concept in cell transplantation. Transplant Proc. 1992 Dec;24(6):2840-1. PMID: 1465965. PDF available below
PMID_1465965 🔗

Update dd 20250115 – proceedings paper PMID: 1465965 added

Citation info : Pittsburgh 1992 Cell Transplant Society • 20240812 • Michel van der Burg • michelvanderburg•com

Minneapolis 1989 Poster | 20240804

September 1989 at a symposium of two international societies in Minneapolis, USA (1) , I presented this first poster on the ‘ invention ‘ of using the UW solution ( the University of Wisconsin organ preservation solution) during pancreatic islet isolation, with the exciting discovery of near-complete purification of islets of Langerhans in the laboratory for transplantation in diabetic patients.

Here the remastered 20240804 edition of that Minneapolis 1989 poster presentation.

The UWS data had been added ad hoc in the poster – that is , it wasn’t part of the accepted abstract. The first proceedings paper on our UWS work was also submitted at this meeting , and was published April ’90 (3).

A previous edition of the remastered poster was published here two years ago (2) but that edition had a different photo print (higher magnification, and without the bar indicator) of the purified islet preparation inserted, not the original image. This has been corrected now. In addition the original line spacing is now restored.

Notes

1) Van der Burg MPM, Gooszen HG, Ploeg RJ, Scherft JP, Field MJ, Guicherit OR, Terpstra JL, Van de Woude FT, Frölich M, Bruijn JA. Comparison of islet isolation techniques in dogs: Over 90% purified islets using UW solution. 4th International Symposium on Organ Procurement and Preservation and 2nd International Congress on Pancreatic and Islet Transplantation, Minneapolis (USA) September 17–22, 1989.

2) Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2022/08/22

3). Van der Burg MPM, Gooszen HG, Ploeg RJ, Scherft JP, Field MJ, Guicherit OR, Terpstra JL, Van de Woude FJ, Frölich M, Bruijn JA. Comparison of islet isolation techniques in dogs: Over 90% purified islets using UW solution. Transplant Proc. 1990 Apr;22(2):795-6. PMID: 2158174.
This paper appears to be not readily available online – a reprint copy is available here PMID_2158174 🔗

PMID_2158174 Download

Updates

20241211 : Minor text updates

20260128 : Added Reference 3 and pdf file of the proceedings paper

Citation info : Minneapolis 1989 Poster | 20240804 | Michel van der Burg | michelvanderburg•com | TakeNode 91d70715-3fb3-4380-906a-0daf1b5ddd6a

PentaStarch UWS Pancreatic Islet Purification • 20240802

Following first introduction of the use UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options.
One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS (7) . Note that Pentastarch is a component of UWS.

The Percoll-UWS poster was posted recently (8)
Here the remastered poster presentation on Pentastarch-UWS :
Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 3 strips) were then glued on site along the perforation, creating the 36/24 inch poster.
Finally the photo print of the purified islets was pasted in the poster at the spot at the venue in Lyon. In Lyon a photo of a dithizone stained Pentastarch purified islet sample was shown. To save time the archive was not searched for that image, and another image of Pentastarch purified islets was inserted in this remastered edition, a reflection contrast micrograph of an ultrathin section of Pentastarch purified islets immuno-stained with gold on insulin in the islet cells.

Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla and now learned to use the app to further correct most conversion issues. Pixelmator Pro was used for additional ‘repair’ and insertion of the photo scan.

Notes

1. Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2022/08/22/minneapolis-1989-poster-uw-solution-islet-isolation-20220822/

2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

6. Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media | URL https://michelvanderburg.com/2022/11/25/pancreatic-islet-transplantation-thesis-repository-leiden-university-20221125/

7. Pentastarch is the synthetic colloid component of UWS (ViaSpan / Belzer solution), a purified fraction (250 kD) of hydroxyethyl starch. Pentastarch powder was used here to increase the Pentastarch concentration and density of the UWS.

8. Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com

Citation info : PentaStarch UWS Pancreatic Islet Purification • 20240802 | Michel van der Burg | michelvanderburg•com | TakeNode d11f1cef-a343-4466-8814-3e52a0261cae