Tag: mouse

Iodixanol-UWS Purification and Culture of Pig Islets Xeno Transplants • Cell Transplant Society 1999 • @1MEMO 20250202


Iodixanol-UWS Purification and Culture of Pig Islets Xeno Transplants • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250202

Slideshow (digitally remastered) oral presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland (1)

The Leiden Islet Laboratory developed a novel Iodixanol-UWS density gradient for purification of the islets of Langerhans from the pancreas in the difficult pig model.

Pig islet isolation from the pancreas of slaughterhouse sows was markedly improved by keeping the islets in the UW donor organ preservation solution (UWS) both during the isolation of islets (using the Liberase enzyme blend) and purification of the islets using our novel Iodixanol-UWS density gradient.

Xeno transplantation of these 1-day-cultured pig islets restored normal blood sugar levels in diabetic mice.

PDF slideshow oral presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland

Iodixanol-UWS Purification and Culture of Pig Islets Xeno Transplants • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250202_2 • TakeNode 8bd79256-78b2-4d8e-8d48-6da41102dc2d • Download file available below 1MEMO_20250202_2

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Notes

1) Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Adult pig islet recovery during Liberase isolation, OptiPrep purification and culture for transplantation in nude mice. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 .

2) Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Adult pig islet recovery during Liberase isolation, OptiPrep purification and culture for transplantation in nude mice (Abstract). Cell Transplant 1999; 8: 185. Download abstract, available below 1MEMO_20250202_3

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Citation info : Iodixanol-UWS Purification and Culture of Pig Islets Xeno Transplants • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250202 • TakeNode f9d1fdc5-21e4-4932-b995-4c97639945fe • URL michelvanderburg.com/2025/02/02/

Fresh vs Cultured Pig Islets for Xenografts • NTV Bootcongres 1998 • @1MEMO 20250201

Fresh vs Cultured Pig Islets for Xenografts • NTV Bootcongres 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250201_1 • TakeNode 71d39e7e-ab5e-42b6-8cf2-a17d464ce132


Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Culture of isolated pig islets pre-xenotransplantation greatly improves the quality and survival of the graft (Kweek van geïsoleerde varkenseilandjes pre-xenotransplantatie verbetert aanzienlijk de kwaliteit en overleving van het transplantaat). Tenth Congress of the Dutch Transplantation Society (Bootcongres 1998), Kerkrade (The Netherlands) April 21–23, 1998.

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EN – Abstract

PRE-TRANSPLANT SHORT-TERM CULTURE OF ISOLATED PIG ISLETS MARKEDLY IMPROVES THE QUALITY AND SURVIVAL OF THE XENOGRAFT
Van der Burg MPM,* Rijkelijkhuizen JKRA, Zwaan RP, and Bouwman E, Leiden University Medical Centre, Leiden, The Netherlands.

Pre-transplant culture of isolated islets reduces the graft’s immunogenicity in various models. Little is known, however, on the effect of culture in pig islet xenotransplantation — probably, because porcine islets have been found difficult to culture. Because pilot transplants of freshly-isolated pig islets in strongly immunosuppressed rats resulted in primary non function, we first studied 1-7 day culture of the isolated pig islets at 37°C in RPMI plus 10% porcine serum. Islets were isolated from large sows (n =6) by an improved method, resulting in intact islets (size 185±16 µm) and no loss during purification, yielding 2448 islets (IEQs)/g with a >95% purity and 90±2% viability as assessed by acridine orange – propidium iodide (AOPI) staining. During culture, however, islet recovery was 24±9% at day 1 and 17±6% at day 7 (NS vs day 1). In order to delineate whether the culture conditions or the quality of freshly isolated islets caused the islet loss during culture, we compared graft survival in nude mice at 1 mo after transplantation under the kidney capsule of ~2500 fresh islets in normoglycemic recipients or ~1000 cultured islets in STZ-diabetic (>20 mM) recipients. After transplanting fresh islets, histology of the kidneys — sectioned every 500 µm — demonstrated substantial scarring and (near-)absence of islets. Cultured islet transplants, by contrast, rendered 5/6 recipients normoglycemic, and showed a substantial mass of well-preserved islets with little scarring at the grafts’ site. Thus, viability by AOPI-staining of fresh islets poorly predicts the survival in vivo and in vitro. A non-immune mediated disintegration of part of the fresh islets may substantially reduce the functional capacity of the graft, both direct by lowering the effective islet dose, and indirect first because scarring may hamper the engraftment of viable tissue, and second because the cellular debris most probably will attract macrophages and induce the release of harmful cytokines.

Citation info : Fresh vs Cultured Pig Islets for Xenografts • NTV Bootcongres 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250201 • URL michelvanderburg.com/2025/02/01/

Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • @1MEMO 20250131

Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250131

Slideshow (digitally remastered) of first talk on pancreatic islet xeno-transplantation following adult pig islet purification in our novel Iodixanol-UWS density gradient. Presented at the 17th Workshop of the AIDSPIT Study Group in Igls, Austria, Tuesday, Jan 27, 1998 (1, 2).

Isolation of porcine pancreatic islets (from the pancreas of large sows) was markedly improved by keeping the islets in the UW donor organ preservation solution (UWS) both during the isolation of islets (using the Liberase enzyme blend) and purification of the islets using a novel Iodixanol-UWS density gradient.
Transplantation of the pig islets (after 1 day storage in culture solution) restored normal blood sugar levels in diabetic mice.


20250131 1

Notes

1) Van der Burg MPM, Zwaan RP, Bouwman E. Markedly improved outcome of adult porcine islet isolation, purification, and culture using Liberase-PI versus collagenase-P, and a novel gradient of Optiprep in University of Wisconsin solution. 17th Workshop of the Study Group on Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation (AIDSPIT) of the European Association for the Study of Diabetes (EASD), Igls (Austria) January 25—27,1998

2) Van der Burg MPM, Zwaan RP, Bouwman E. Markedly improved outcome of adult porcine islet isolation, purification, and culture using Liberase-PI versus collagenase-P, and a novel gradient of Optiprep in University of Wisconsin solution (Abstract). Horm Metab Res 1998; 30(1): A23 . DOI: 10.1055/s-2007-978834 .
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Citation info : Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250131 • TakeNode 1f185515-7aeb-44ac-b721-2cec76df67b7 • URL michelvanderburg.com/2025/01/31/

Viability of Fresh vs Cultured Pig Islets for Transplants • Cell Transplant Society 1999 Montreux • 20241204


Oral presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland

A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model of islet isolation. Viability of freshly isolated islets is not guaranteed.
After short-term storage of the islets in culture, pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.

1-Day Cultered Islets (extra slide) • @1MEMO 20241204_1

Slideshow (pdf)

Viability of Fresh vs Cultured Pig Islets for Transplants • Montreux Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241204_2 • TakeNode e745d625-27c2-4eb8-b4c2-4300623c3303 • File 1MEMO_20241204_2

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Rijkelijkhuizen JKRA, Bouwman E, Van der Burg MPM. Viability of fresh vs cultured pig islets for transplants. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999

Other Presentations in Montreux

Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Adult pig islet recovery during Liberase isolation, OptiPrep purification and culture for transplantation in nude mice. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 . The Sydney 1999 presentation of this work has been posted – @1MEMO 20241203

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 . The Igls 1999 presentation of this work has been posted – @1MEMO 20241202

Citation info : Viability of Fresh vs Cultured Pig Islets for Transplants • Cell Transplant Society 1999 Montreux • Michel van der Burg • Miracles.Media • @1MEMO 20241204 • TakeNode 3ba1c894-eb71-4c3f-89aa-198c48cd480c • URL michelvanderburg.com/2024/12/04/

Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • 20241203

Slides oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model. The pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.

Slideshow (pdf) oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241203_2 • TakeNode 5f53ae49-6fb8-4bef-a434-ac2ce8151c2a • File 1MEMO_20241203_2

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Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999

Citation info : Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • 1MEMO 20241203 • TakeNode ea1f1abd-6227-4ae6-985b-f8188310cd24 • URL michelvanderburg.com/2024/12/03/