Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109_1 • TakeNode 75edbb4e-4360-43b8-8783-363ffd34c0ef
Poster (1) presented at the 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991. The poster was originally created on a Macintosh computer Oct 6, 1991, using the MacDraw II application, with graphs from Cricket Graph. That 1991 poster was remastered today in LibreOffice.
We presented 2 posters at the 5th ESOT, this poster (1), and another one on the effects of denervation with pancreas transplantation (2).
Two years after the 4th ESOT (in Barcelona, Nov 1989) where we introduced the UW-Solution as an islet isolation solution before density gradient purification, we now had a talk at this 5th ESOT on the use of the UW-Solution as the islet isolation solution, both before purification, and also during the purification in an innovative density gradient (3) — the foundation for current clinical purification methods. The talk was recorded on tape (except for the intro), and will be posted here soon too.
Notes
1) Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.
2) Guicherit OR, Van der Burg MPM, Lemkes HHPJ, Frölich M, Gooszen HG. Pancreatic denervation causes reduced insulin sensitivity. 5 th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) Oct 7–10 1991.
3) Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.
Citation info : Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109 • URL michelvanderburg.com/2025/01/09/
Greek Poster • Michel van der Burg • Miracles•Media • 20241009
Funny. LibreOffice Vanilla Issue.
Greek characters appeared in this poster originally designed with english text and produced on a Macintosh computer and Imagewriter dotmatrix printer, September 3, 1990, using the MacDraw II application, with graphs from Cricket Graph. That 1990 Mac file was remastered today in LibreOffice Vanilla (other minor issues in graphs not corrected). Generally LibreOffice does a great job with these ancient files. I recently posted several of such posters at site michelvanderburg.com. However, some of the posters retrieved from my archive show up with Greek characters when opened in LibreOffice, like this poster produced in English (with roman characters). Those problematic posters have in common that the Apple Chicago font was used – the interface font in Apple’s early versions of Mac OS from 1984 to 1997. Clearly LibreOffice cannot handle this Chicago font correctly. I have not found a solution for this. Text in other posters, produced with Helvetica, had only minor issues in LibreOffice, Helvetica italics text appeared as regular Times.
The original poster and abstract (below) were presented at the 8th International Symposium on Gastrointestinal Hormones, in Timmendorfer Strand Baltic Sea (FRG) September 4–8, 1990.
Abstract
Contribution of gut factors to canine isolated islet function M.P.M. van der Burg, O.R. Guicherit, J.B.M.J. Jansen, C.B.H.W. Lamers, J.P. Scherft, M. Frölich, H.G. Gooszen. Departments of Surgery, Gastroenterology. Endocrinology and Cel Biology. University Hospital, Leiden, The Netherlands. Techniques for isolating islets of Langerhans from the large mammalian pancreas have improved to the point where islet transplantation as a therapeutical approach to human diabetes has become more realistic. The quality of metabolic control is however largely unknown. We studied canine isolated-islet function both in vivo after autotransplantation, as well as in vitro by perifusion. Five normal dogs underwent total pancreatectomy. Islets were isolated from the excised pancreas by collagenase digestion, dispersion and purification with filtration and density gradients. Isolated islets were autotransplanted into the spleen of the dog by retrograde venous infusion. Graft function was assessed up to 3 mo by determining the glucose and insulin response to an intravenous glucose injection (IVGTT), i.v. arginine injection during 35 mM glucose clamp (AT), and a meal. In addition (n = 4) the in vitro insulin response of overnight cultured canine islets was studied by perifusion with cholecystokinin (CCK-33) and glucose-dependent insulinotropic polypeptide (GIP). The islet dose at transplantation ranged from 3500 – 13000 islets/kg b.w. One animal became overtly hyperglycemic (fasting glucose 18 mM) within 7 days after receiving 3500 islets/kg b.w. The other grafts (> 6000 islets/kg b.w.) were successful (fasting glucose <7mM) but demonstrated, compared to pre-operative values, a 50% reduced glucose clearance and insulin response at IVGTT, and a 90% reduced insulin secreting capacity at AT. Postprandially hyperglycemia (~ 10 mM) and in contrast to i.v. glucose and arginine, a normal insulin response was observed. The in vitro insulin response at 7.5 mM glucose to equimolar (0.1-1- 10 nM) concentrations of either CCK, GIP or both, demonstrated a sustained dose-response to GIP (up to 5x basal values) and a significant albeit small and transient effect of CCK as of 1 nM. No synergistic effect was observed. In dogs ‘one-to-one’ transplantation was successful in 4/5 recipients of isolated islets. The difference in the effect of islet transplantation on the insulin response to intravenous glucose or – arginine and a meal, may be related to the postprandial, hyperglycemia enhanced, activation of the entero-insular axis, especially GIP.
Van der Burg MPM, Guicherit OR, Jansen JBMJ, Lamers CBHW, Scherft JP, Frölich M, Gooszen HG. Contribution of gut factors to canine isolated islet function (Abstract). Digestion 1990; 46 (Suppl 1): 14–15. https://doi.org/10.1159/000200356
PDF abstract shown below – file 20241009_1_Digestion.pdf
Citation info : Greek Poster • Michel van der Burg • Miracles•Media • 20241009 • URL michelvanderburg.com/2024/10/09 | TakeNode 10afaddd-08b7-4dcd-ad7a-43228420ad59
Function after intrasplenic transplantation of islets of Langerhans in dogs.
The effect of gut hormones GLP-1 , GIP , and CCK-33 together with glucose on the insulin secretion capacity of isolated islets tested during storage of the islets in the laboratory (in vitro by perifusion), and after transplantation (in vivo).
Dutch poster (1) presented at the Surgeon Days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992.
An English edition of this presentation was presented in 1991, without the GLP-1 data (2) and posted again Sep 6, 2024 (3).
The poster was originally designed and produced on a Macintosh computer and Imagewriter dotmatrix printer, May 1992, using the MacDraw II application, with graphs from Cricket Graph. That 1992 Mac file was remastered today in LibreOffice Vanilla.
Notes
1. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Functie na intralienale transplantatie van eilandjes van Langerhans in de hond ( Function after intrasplenic transplantation of islets of Langerhans in dogs ). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992.
2. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
4. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide, and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Peptides 1995; 60: 61–67.
Citation info : Gut factors controlling pancreatic islets • 20240910 | Michel van der Burg | michelvanderburg•com | TakeNode 56ddc057-ffe9-4b70-bd33-180ea4b07877
The effect of gut hormones together with glucose on the insulin secretion capacity of isolated islets tested during storage of the islets in the laboratory, and after transplantation.
CCK and GIP control of canine isolated islet function.
Poster (1) presented at the 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
The poster was originally designed and produced on a Macintosh computer and Imagewriter dotmatrix printer, June 3, 1991, using the MacDraw II application, with graphs from Cricket Graph. That 1991 Mac file was remastered today in LibreOffice Vanilla.
Notes
1. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
Citation info : Gut feelings of pancreatic islets • 20240906 | Michel van der Burg | michelvanderburg•com | TakeNode 5e52cddc-469d-4f91-bd23-e833566be41e
Following first introduction of the use UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options.
One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS (7) . Note that Pentastarch is a component of UWS.
The Percoll-UWS poster was posted recently (8)
Here the remastered poster presentation on Pentastarch-UWS :
Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 3 strips) were then glued on site along the perforation, creating the 36/24 inch poster.
Finally the photo print of the purified islets was pasted in the poster at the spot at the venue in Lyon. In Lyon a photo of a dithizone stained Pentastarch purified islet sample was shown. To save time the archive was not searched for that image, and another image of Pentastarch purified islets was inserted in this remastered edition, a reflection contrast micrograph of an ultrathin section of Pentastarch purified islets immuno-stained with gold on insulin in the islet cells.
Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla and now learned to use the app to further correct most conversion issues. Pixelmator Pro was used for additional ‘repair’ and insertion of the photo scan.
2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
7. Pentastarch is the synthetic colloid component of UWS (ViaSpan / Belzer solution), a purified fraction (250 kD) of hydroxyethyl starch. Pentastarch powder was used here to increase the Pentastarch concentration and density of the UWS.
8. Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com
Citation info : PentaStarch UWS Pancreatic Islet Purification • 20240802 | Michel van der Burg | michelvanderburg•com | TakeNode d11f1cef-a343-4466-8814-3e52a0261cae
Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e
Following first introduction of the use of UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options. One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS . Note that Pentastarch is a component of UWS.
Here one of these poster presentations, on Percoll-UWS : Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application, inserted with a bar graph prepared in the Cricket Graph app. The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 5 strips) were then glued on site along the perforation, creating a 36/41 inch poster. Finally the RCM photo print of the purified human islets was pasted in the poster at the spot at the venue in Lyon.
Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla (open source app) with nice results. Only minor issues appeared with text spacing of the top row of the table , and the caption in the photo section. Some color is showing in the graphs , not evident on my grayscale equipment in the 90s. …. The poster image of 20240731 has been replaced by a remastered image , see Note Update 20240808 below.
2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
Following the first publication here last month (20240731) of the original 1991 MacDraw poster opened (converted) in LibreOffice Vanilla (1), I learned how to do some editing in the LibreOffice app to further restore poster layout details such as line spacing, and font style. An archive photo of another poster presentation in Pittsburgh in 1992 (that will be posted here soon) guided this restoration.
Current workflow : 1) opening the MacDraw II file in LibreOffice Vanilla 2) editing in in LibreOffice Vanilla , saving as LibreOffice Vanilla file (OpenDocument Drawing – extension odg). 3) opening the photo tiff image in the Mac’s Preview app, and copying the displayed image to the clipboard 4) pasting the clipboard image in the LibreOffice doc , and dragging to adjust size and location 5) exporting the file as PDF (image resolution 300 DPI ; PDF/A-1b ; PDF/UA). 6) Converting the PDF in the Preview app to TIFF 144 pixels/inch. 7) Adding caption , metadata etc with JPEG conversion in PhotoMill.
This 20240731 post now has this updated 20240808 – poster image.
Citation info : Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com
Lancet Letter 1989 UW Solution Islet Isolation | 20220823 | Michel van der Burg | michelvanderburg•com | June 1989 preparing first submission for publication as letter in the Lancet medical journal the new concept of using the UW solution ( the University of Wisconsin organ preservation solution) for isolation and complete purification of islets of Langerhans in the laboratory for transplantation in diabetic patients. Later that year that exciting discovery was presented at meetings in Minneapolis, USA, and Barcelona, Spain. Details in yesterday’s post. Submission for publication as a letter in the Lancet had had no succes 😉
Final draft text produced on my personal Macintosh ED and Imagewriter-II matrix printer with Geneva font.
