Tag: incretin

Glycemic control mechanisms after canine islet autografting • IPITA 1995• @1MEMO 20251204

Glycemic control mechanisms after canine islet autografting • IPITA 1995 • Michel van der Burg • Miracles.Media • @1MEMO 20251204_1 • Talk, Tuesday June 20, 1995 at 10:30, 5th IPITA, Eden Roc Resort Hotel, Miami Beach, FL (USA) • Digitally remastered by author, publisher, Michel van der Burg • TakeNode 5227c200-e029-4406-b344-15350fe3b93d • #GLP1 #GIP #diabetes #islets #transplant

Pioneer work on GLP-1 and GIP incretin hormones

Slideshow (digitally remastered) of my talk Tuesday June 20, 1995 at 10:30, at the 5th International Congress on Pancreas and Islet Transplantation (IPITA), in Miami Beach, FL (USA). My first talk at a world congress presenting the outcome of all our detailed metabolic studies in our preclinical model of canine islet autotransplantation , including our pioneer work on the incretin effects of the gut hormones GLP-1 (glucagon-like peptide-1) and GIP (Glucose-dependent Insulinotropic Polypeptide) — now, in 2025, well-known from the drugs that mimic the action of these natural hormones, to treat type 2 diabetes and obesity, by stimulating insulin release, improving blood sugar control, and reducing appetite.

May 1992 we were the first to show direct potentiation of the insulin response from isolated islets by (near-)physiological doses of both the gut hormones GLP-1 and GIP during perifusion (in vitro) of canine islets at 7.5-10 mM glucose levels (1,2), and January 1994 we next confirmed the incretin effect of a (near-)physiological dose of GLP-1 after canine islet transplantation , in a poster presented at the 13th Workshop of the AIDSPIT (aka AIDPIT) Study Group , Igls, Austria, January 23–25, 1994 (3).

Background of the work was posted earlier this year (4) and the work was published Nov 1994 in my PhD thesis Pancreatic islet transplantation , available online (5).


Welcome Reception 5th IPITA on Sunday 18 June 1995, at the congress venue , the Eden Roc Resort Hotel, in Miami Beach, FL (USA) • Michel van der Burg • Miracles.Media • @1MEMO 20251204_2 • TakeNode 8eb04df1-8280-4de3-a206-4b63413c3025

June 1995, 5th IPITA Talk

Tuesday June 20, 1995 at 10:30, at the 5th International Congress on Pancreas and Islet Transplantation (IPITA), in Miami Beach, FL (USA), was my first talk at a world congress presenting the outcome of all our detailed metabolic studies in the canine islet transplants , including the incretin effect of GLP-1, and also our findings with isolated canine islets during perifusion, in vitro, of the direct insulinotropic effects of the gut hormones GIP and GLP-1 (6) . That slideshow presentation (digitally remastered*) is posted here (1MEMO 20251204_1).

The proceedings paper delivered at the 5th IPITA is included in the Notes (7) , as well as the referenced previous papers (8,9)

Notes

1. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Functie na intralienale transplantatie van eilandjes van Langerhans in de hond ( Function after intrasplenic transplantation of islets of Langerhans in dogs ). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992. In : Gut factors controlling pancreatic islets • 20240910 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/09/10/gut-factors-controlling-pancreatic-islets-20240910/

2. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

3. Metabolic control after canine islet transplantation. M.P.M. van der Burg, P.T.R. van Suylichem, O.R. Guicherit, J.H.M. van Deijnen, M. Frölich, and H.G. Gooszen (Universities of Leiden & Groningen, The Netherlands). Poster presented at the 13th Workshop of the AIDSPIT Study Group , Igls, Austria, January 23–25, 1994 • In : GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • Michel van der Burg • Miracles.Media • @1MEMO 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • URL https://michelvanderburg.com/2025/11/14

4. Background of this work was posted in Dutch Islets Transplant Team • @1MEMO 20250125 • URL https://michelvanderburg.com/2025/01/25/dutch-islets-transplant-team-1memo-20250125/

5. Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Dec 4th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .

6. Oral presentation : Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. 5th International Congress on Pancreas and Islet Transplantation (IPITA), Miami Beach (USA) June 20, 1995.

7. 5th IPITA Proceedings publication : Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. Transplant Proc. 1995 Dec;27(6):3187-8. PMID: 8539903. PDF available below PMID_8539903

PMID_8539903Download
Referenced previous publications :

8. Van der Burg MPM, Gooszen HG. The metabolic efficiency of islet grafts: an overview. In: Islet transplantation — current status of clinical application and experimental results. Hesse UJ , Pichlmaier H, Eds., Lengerich Germany, Wolfgang Pabst Verlag, 1992, p.93–99. Pabst hash code 0383b3abad3ae774b146ea0425c2227a PDF available below 1MEMO_20251204_4

1MEMO_20251204_4Download

9. Van der Burg MPM, Guicherit OR, Scherft JP, Lemkes HHPJ, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors (Abstract). Neth J Med 1992; 41: A25. PDF available below 1MEMO_20251204_3

1MEMO_20251204_3Download
Other previous publications :

10. Van der Burg MPM, Guicherit OR, Van Suylichem PTR, Frölich M, Van Deijnen JHM, Gooszen HG. Importance of the entero-insular axis for islet graft function (Abstract). Cell Transplant 1994; 3: 257. Abstract – Second International Congress of the Cell Transplant Society May 1–4, 1994 Hyatt Regency Hotel • Minneapolis, Minnesota . https://doi.org/10.1177/096368979400300302 (Abstracts published , posters not presented). PDF available below 10.1177_096368979400300302

10.1177_096368979400300302Download

11. Chapter 8, Doctoral Thesis, Leiden University, November 1994 : Metabolic control after intrasplenic islet autotransplantation in dogs: ß-cell secretory capacity, insulin action, and the enteroinsular axis. Michael P.M. van der Burg, Paul T.R. van Suylichem, Onna R. Guicherit, Marijke Frölich, Herman H.P.J. Lemkes, and Hein G. Gooszen. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 8). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .
Direct link to file Chapter 8 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486615/view .

12. Doctoral Thesis, Leiden University, November 1994 (Chapter 6) : lnsulinotropic properties of cholecystokinin, gastric inhibitory polypeptide, and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Michael P.M. van der Burg, Onna R. Guicherit, Marijke Frölich and Hein G. Gooszen. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 6). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .
Direct link to file Chapter 6 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486613/view

Full papers

13. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

14. Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glucoregulation after canine islet transplantation: contribution of insulin secretory capacity, insulin action, and the entero-insular axis. Cell Transplant. 1997 Sep-Oct;6(5):497-503. doi: 10.1177/096368979700600509. PMID: 9331501. PDF link https://pure.rug.nl/ws/portalfiles/portal/58359942/096368979700600509.pdf

* 1MEMO 20251204_1 . Digitally remastered presentation, using the images from the original slides handout, and the handout of the text spoken during the original presentation , now read using my personal synthesized voice.

#GLP1 #GIP #diabetes #islets #transplant

Citation info : Glycemic control mechanisms after canine islet autografting • IPITA 1995 • Michel van der Burg • Miracles.Media • @1MEMO 20251204 • URL https://michelvanderburg.com/2025/12/04

GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • @1MEMO 20251114

Poster 13th Workshop of the AIDSPIT Study Group , Igls (Austria) January 23–25, 1994• Digitally remastered by author, publisher, M.P.M. van der Burg • Miracles•Media • 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • #GLP1

GLP-1 blood sugar regulation in islet transplants

GLP-1 (glucagon-like peptide-1) is now well-known from the anti-diabetic drugs that mimic the action of this natural hormone GLP-1, to treat type 2 diabetes and obesity. They work by stimulating insulin release, reducing glucagon secretion, slowing stomach emptying, and suppressing appetite, which leads to lower blood sugar and weight loss.

May 1992 we were the first to show direct potentiation of the insulin response from isolated islets by a (near-)physiological dose of the gut hormone GLP-1 during perifusion (in vitro) of canine islets at 7.5-10 mM glucose levels (1,2).

Jan 1994 we next showed this incretin effect of GLP-1 also after islet transplantation (in vivo, in dogs) in this poster (3) and abstract (4) presented at the 13th Workshop of the AIDSPIT (aka AIDPIT) Study Group , Igls, Austria, January 23–25, 1994.

Background of the work was posted earlier this year (5) and the work was published Nov 1994 in my PhD thesis Pancreatic islet transplantation , available online (6).

Notes

1) Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Function after intrasplenic transplantation of islets of Langerhans in dogs (translated from dutch: Functie na intralienale transplantatie van eilandjes van Langerhans in de hond). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992. In : Gut factors controlling pancreatic islets • 20240910 | Michel van der Burg | michelvanderburg•com URL https://michelvanderburg.com/2024/09/10/gut-factors-controlling-pancreatic-islets-20240910/

2) van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

3) Metabolic control after canine islet transplantation. M.P.M. van der Burg, P.T.R. van Suylichem, O.R. Guicherit, J.H.M. van Deijnen, M. Frölich, and H.G. Gooszen (Universities of Leiden & Groningen, The Netherlands). Poster presented at the 13th Workshop of the AIDSPIT Study Group , Igls, Austria, January 23–25, 1994 •

4) Metabolic control after canine islet transplantation. Van der Burg M.P.M., Guicherit O.R., van Suylichem P.T.R., Frölich M., van Deijnen J.H.M. , Gooszen H.G. (Leiden, The Netherlands; Groningen, The Netherlands). Horm metab Res 1994; 26: 63. (Abstract). View/Download file 1MEMO_20251113_2

1MEMO_20251113_2Download

5) Background of this work was posted in Dutch Islets Transplant Team • @1MEMO 20250125 • URL https://michelvanderburg.com/2025/01/25/dutch-islets-transplant-team-1memo-20250125/

6) Michael P.M. van der Burg. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Faculty of Medicine, Leiden University Medical Center (LUMC) , Leiden University), Boskoop: M.P.M. van der Burg | Miracles.Media (ISBN electronic, pdf, 9789080216402 ; ISBN print 9789080216419, 9080216410), 1994: 192 p. URL Thesis Repository Leiden University https://hdl.handle.net/1887/3486604

PM – Recording of the 13th AIDSPIT seminar on lessons from clinical islet transplantation was posted yesterday (7).

7) Clinical Islet Transplantation 13th AIDSPIT • @1MEMO 20251113 • URL https://michelvanderburg.com/2025/11/13/clinical-islet-transplantation-13th-aidspit-1memo-20251113/

#GLP1 #diabetes #islets #transplant #AIDSPIT

Updates

20251127 title updated – insert : AIDSPIT 1994

Citation info : GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • Michel van der Burg • Miracles.Media • @1MEMO 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • URL https://michelvanderburg.com/2025/11/14

Dutch Islets Transplant Team • @1MEMO 20250125

Islets isolated April 18, 1989 using the UW-Solution • Miracles.Media • 20250103_1 • TakeNode eeb55729-c02b-4f73-ab0e-9f71b65bf85e

Rightaway, April 1989, when we obtained these highly purified canine islets of Langerhans by introducing the UW-Solution for isolating the islets from pancreases (1,2,3), we started using these islets to study the importance of gut factors for islet transplants — the capacity of the isolated islets to secrete insulin during stimulation with glucose, other nutrients, and potentially gut hormones ( ‘incretins’).

Isolated islets in perifusion chambers

Isolated islets, not transplanted, were examined in the Leiden Islet Laboratory. The insulin secretion of these islets was measured, while the islets were housed in plastic micro-chambers , that were flushed with solutions containing various levels of glucose and gut hormones , mimicking the blood levels as found following meals in islet transplants (4).

Monitoring pancreatic islets in perifusion • Michel van der Burg • Miracles.Media • @1MEMO 20240911

Autotransplantation of islets

A few months later , Summer 1989, we performed a series of transplants of isolated canine islets (5) , and likewise focussed our research on the importance of gut factors in metabolic control by the islet grafts, and therefore introduced meal tests — apart from a series of intravenous tests. Both simple and convenient, such meal tests proofed to be also an excellent way to study both the capacity of the transplanted islets to secrete insulin, as well as the contribution of gut hormones – in the so-called ‘entero-insular axis’ (6).

Autotransplantation of islets in the spleen • Michel van der Burg • Miracles.Media • @1MEMO 20250125_1 • Left : Canine pancreas with two cannulas in the pancreatic ducts (for infusion of collagenase solution) , with the blood vessels clamped at the time of removal of the gland. Not shown here: After removal of the gland the collagenase solution is infused , and the pancreatic islets are isolated within a few hours. Right : Next the purified islets are returned by infusion in the spleen of the same animal (auto-transplantation). Drawings by medical artist J. Wetselaar-Whittaker — with my hand posing for the injection drawing 😉

Contribution of gut factors to canine isolated islet function ?

Spring 1990 our first results could be presented of these sophisticated islet function tests upto 3 months after islet transplantation, together with our first findings of the direct effects of the gut hormones CCK and GIP on the isolated islets in the perifusion system in the Leiden Islet Lab.

Spring meeting 1990 of the Dutch Society for Gastroenterology, Veldhoven, Netherlands • Michel van der Burg • Miracles.Media • @1MEMO 20250125_2

Here the proceedings presented at the 1990 spring meeting of the Dutch Society for Gastroenterology (7).

Dutch Islets Transplant Team

October 8, 1990, Paul van Suylichem, from the Groningen islet research group, visited his alma mater again, the Leiden University on the occasion of the 3rd Symposium Experimental Surgical Research (SEOHS).
I was presenting results at the symposium on metabolic control upto 3 mo after the succesful canine islet transplants – islets isolated using the UW-Solution – known as Viaspan for the cold storage of donor organs (8,9).
Paul had started together with me in 1985 building the Leiden Islet Lab (10), that I continued from 1986 after Paul left together with project leader Reinout van Schilfgaarde starting the Groningen islet group (11).
I took the chance to invite Paul for a visit together that day in the nearby Leiden Islet Lab , for a closer look at our latest work on canine islet isolation, transplantation, and the new Percoll-UWS purification gradients (12) — and took this picture of Paul examining an islet prep at the microscope in the lab , with the Acusyst perifusion system in the background.
In late 1990, the Groningen islet team with Paul would also start this preclinical model of canine islet isolation and transplantation.

Paul van Suylichem (Groningen Islet Team) in Leiden Islet Laboratory, October 8, 1990 • Photo by Michel van der Burg • Miracles.Media • @1MEMO 20250125_3

A year later, Oct 1991, during the 5th Congress of the European Society for Organ Transplantation Congress (5th ESOT) in Maastricht (12), I learned from Paul’s co-worker Jan-Erik van Deijnen that the Groningen team, after initially several failed transplant attempts, had finally succeeded in the last 5 transplants in a row. They had mastered the technique and had started to complete this work with histology of the transplants.

We, in Leiden, had made plans earlier that summer to expand our small group of islet transplants, with a second series of transplants with a view to expanding our research on metabolic control in islet transplantation, with new sophisticated research on the effect on insulin secretion of the newly discovered gut hormone GLP-1. At the same time we would also be able to test our efficient new technique of isolation and purification of the islets in that transplantation setting.

A few days after this 5th ESOT congress it seemed better to me to refrain from new transplants in more animals, and I proposed to expand our sophisticated, functional research with research on the Groningen canine islet transplants.

Later that month, Oct 1991, islet perifusion experiments with the gut hormone GLP-1 were started (13,14,15).

And… November 1991, Paul van Suylichem’s research associate Annemiek Weemaes brought the Groningen canine transplants to Leiden, for our very successful collaborative project , with the planned additional studies in the combined Leiden and Groningen group of transplants, examining additionally insulin action (sensitivity) and the ‘incretin’ effect (stimulation of insulin secretion) of the gut hormone GLP-1. Experiments we completed in 1992 (14,16,17,18).

Notes

IMAGE 20250103_1
Islets isolated April 18, 1989 using the UW-Solution • Miracles.Media • 20250103_1 • TakeNode eeb55729-c02b-4f73-ab0e-9f71b65bf85e

1) Pancreatic Islet Isolation With UW Solution • A New Concept • Michel van der Burg • Miracles.Media • @1MEMO 20241224 • URL https://michelvanderburg.com/2024/12/24/pancreatic-islet-isolation-with-uw-solution-a-new-concept-20241224/

2) Pancreatic Islet Isolation in UW Solution & Transplantation • ESOT 1989 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20241229 • URL michelvanderburg.com/2024/12/29/

3) Pancreatic Islets UWS Series 1989 • Kodak PhotoCD • Michel van der Burg • Miracles.Media • @1MEMO 20250103 • URL michelvanderburg.com/2025/01/03/

4) Monitoring pancreatic islets in perifusion • Michel van der Burg • Miracles.Media • @1MEMO 20240911 • TakeNode fdf6f65c-28b7-4936-8c23-83225e6f2ff0 • URL https://michelvanderburg.com/2024/09/11/

IMAGE 20240911
Monitoring pancreatic islets in perifusion • Michel van der Burg • Miracles.Media • @1MEMO 20240911 • TakeNode fdf6f65c-28b7-4936-8c23-83225e6f2ff0

5) Islet Transplantation Breakthrough in Leiden University Hospital • 20240830 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/08/30/

6) Entero Insular Axis • Michel van der Burg • Miracles.Media • @1MEMO 20250123 • URL michelvanderburg.com/2025/01/23/

IMAGE 20250125_1
Autotransplantation of islets in the spleen • Michel van der Burg • Miracles.Media • @1MEMO 20250125_1 • Left : Canine pancreas with two cannulas in the pancreatic ducts (for infusion of collagenase solution) , with the blood vessels clamped at the time of removal of the gland. Not shown here : After removal of the gland the collagenase solution is infused , and the pancreatic islets are isolated within a few hours. Right : Next the purified islets are returned by infusion in the spleen of the same animal (auto-transplantation). Drawings by medical artist J. Wetselaar-Whittaker — with my hand posing for the injection drawing 😉 • TakeNode da74e6a2-e21d-4690-afe5-356949221658

IMAGE 20250125_2
Spring meeting 1990 of the Dutch Society for Gastroenterology, Veldhoven, Netherlands • Michel van der Burg • Miracles.Media • @1MEMO 20250125_2 • TakeNode 65cb9de6-a111-4636-8766-d586b001d73e

7) Van der Burg MPM, Guicherit OR, Jansen JBMJ, Lamers CBHW, Scherft JP, Gooszen HG.
Contribution of gut factors to canine isolated islet function? Spring meeting of the Dutch Society for Gastroenterology (NVGE), Veldhoven (The Netherlands) March 23–24, 1990. The proceedings pdf (1MEMO_20250125_2) is available for download below.

1MEMO_20250125_2🔗Download

Five years later the Dutch Society for Gastroenterology awarded the Glaxo Wellcome Gastrointestinal Research Award for this research (Ph.D. thesis “Pancreatic islet transplantation”) at the Fall meeting of the Dutch Society for Gastroenterology (NVGE), Veldhoven, The Netherlands, October 5, 1995).

Glaxo Wellcome Gastroenterology Research Award, Oct 5, 1995, Dutch Society for Gastroenterology, Veldhoven, Netherlands • Michel van der Burg • Miracles.Media • @1MEMO 20250125_4 • TakeNode 3152bca6-c98e-4900-89bf-d3e241f14405

8) Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Bruijn JA, Frölich M, Prins FA, Gooszen HG. Successful canine pancreatic islet transplantation using ViaSpan. 3rd Symposium Experimental Surgical Research (SEOHS), Leiden (The Netherlands) October 8, 1990.

9) Van der Burg MPM, Gooszen HG, Ploeg RJ, Guicherit OR, Scherft JP, Terpstra JL, Frölich M. Over 90% purified canine isolated pancreatic islets using UW solution (Abstract). Neth J Surg 1990; 42: 30. Pdf abstract (1MEMO_20250125_5) is available for download below.

1MEMO_20250125_5🔗Download

10) Leiden Islet Laboratory History • 20240803 | Michel van der Burg | URL michelvanderburg.com/2024/08/03/

11) Cradle of Islet Transplants • 20240829 • Michel van der Burg • URL https://michelvanderburg.com/2024/08/29/

12) Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/01/14/

IMAGE 20250125_3
Paul van Suylichem (Groningen Islet Team) in Leiden Islet Laboratory, October 8, 1990 • Photo by Michel van der Burg • Miracles.Media • @1MEMO 20250125_3 • TakeNode e8ce7630-4e75-402b-a4c2-9b007a8dd61b

13) Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Functie na intralienale transplantatie van eilandjes van Langerhans in de hond ( Function after intrasplenic transplantation of islets of Langerhans in dogs ). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992. In : Gut factors controlling pancreatic islets • 20240910 | Michel van der Burg | michelvanderburg•com

14) Michael P.M. van der Burg. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Faculty of Medicine, Leiden University Medical Center (LUMC) , Leiden University), Boskoop: M.P.M. van der Burg | Miracles.Media (ISBN electronic, pdf, 9789080216402 ; ISBN print 9789080216419, 9080216410), 1994: 192 p. URL Thesis Repository Leiden University https://hdl.handle.net/1887/3486604

15) van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

16) Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. 5th International Congress on Pancreas and Islet Transplantation (IPITA), Miami Beach (USA) June 18–22, 1995.

17) van der Burg MPM, van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. Transplant Proc. 1995 Dec;27(6):3187-8. PMID: 8539903. Download (PMID_8539903) below .

PMID_8539903🔗Download

18) van der Burg MPM, van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glucoregulation after canine islet transplantation: contribution of insulin secretory capacity, insulin action, and the entero-insular axis. Cell Transplant. 1997 Sep-Oct;6(5):497-503. doi: 10.1177/096368979700600509. PMID: 9331501. Download Free PDF https://hdl.handle.net/11370/59231eab-6091-415d-8b2b-4e5e6508a978

Citation info : Dutch Islets Transplant Team • Michel van der Burg • Miracles.Media • @1MEMO 20250125 • URL michelvanderburg.com/2025/01/25/

Islet Transplants Performance 5th ESOT 1991 • @1MEMO 20250109

Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109_1 • TakeNode 75edbb4e-4360-43b8-8783-363ffd34c0ef

Poster (1) presented at the 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991. The poster was originally created on a Macintosh computer Oct 6, 1991, using the MacDraw II application, with graphs from Cricket Graph. That 1991 poster was remastered today in LibreOffice.

We presented 2 posters at the 5th ESOT, this poster (1), and another one on the effects of denervation with pancreas transplantation (2).

Two years after the 4th ESOT (in Barcelona, Nov 1989) where we introduced the UW-Solution as an islet isolation solution before density gradient purification, we now had a talk at this 5th ESOT on the use of the UW-Solution as the islet isolation solution, both before purification, and also during the purification in an innovative density gradient (3) — the foundation for current clinical purification methods. The talk was recorded on tape (except for the intro), and will be posted here soon too.

Notes

1) Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.

2) Guicherit OR, Van der Burg MPM, Lemkes HHPJ, Frölich M, Gooszen HG. Pancreatic
denervation causes reduced insulin sensitivity. 5 th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) Oct 7–10 1991.

3) Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.

Citation info : Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109 • URL michelvanderburg.com/2025/01/09/

Greek Poster • 20241009

Greek Poster • Michel van der Burg • Miracles•Media • 20241009

Funny. LibreOffice Vanilla Issue.

Greek characters appeared in this poster originally designed with english text and produced on a Macintosh computer and Imagewriter dotmatrix printer, September 3, 1990, using the MacDraw II application, with graphs from Cricket Graph.
That 1990 Mac file was remastered today in LibreOffice Vanilla (other minor issues in graphs not corrected). Generally LibreOffice does a great job with these ancient files. I recently posted several of such posters at site michelvanderburg.com.
However, some of the posters retrieved from my archive show up with Greek characters when opened in LibreOffice, like this poster produced in English (with roman characters).
Those problematic posters have in common that the Apple Chicago font was used – the interface font in Apple’s early versions of Mac OS from 1984 to 1997. Clearly LibreOffice cannot handle this Chicago font correctly. I have not found a solution for this.
Text in other posters, produced with Helvetica, had only minor issues in LibreOffice, Helvetica italics text appeared as regular Times.

The original poster and abstract (below) were presented at the 8th International Symposium on Gastrointestinal Hormones, in Timmendorfer Strand Baltic Sea (FRG) September 4–8, 1990.

Abstract

Contribution of gut factors to canine isolated islet function
M.P.M. van der Burg, O.R. Guicherit, J.B.M.J. Jansen, C.B.H.W. Lamers, J.P. Scherft, M. Frölich, H.G. Gooszen.
Departments of Surgery, Gastroenterology. Endocrinology and Cel Biology. University Hospital, Leiden, The Netherlands.
Techniques for isolating islets of Langerhans from the large mammalian pancreas have improved to the point where islet transplantation as a therapeutical approach to human diabetes has become more realistic. The quality of metabolic control is however largely unknown. We studied canine isolated-islet function both in vivo after autotransplantation, as well as in vitro by perifusion.
Five normal dogs underwent total pancreatectomy. Islets were isolated from the excised pancreas by collagenase digestion, dispersion and purification with filtration and density gradients. Isolated islets were autotransplanted into the spleen of the dog by retrograde venous infusion. Graft function was assessed up to 3 mo by determining the glucose and insulin response to an intravenous glucose injection (IVGTT), i.v. arginine injection during 35 mM glucose clamp (AT), and a meal. In addition (n = 4) the in vitro insulin response of overnight cultured canine islets was studied by perifusion with cholecystokinin (CCK-33) and glucose-dependent insulinotropic polypeptide (GIP).
The islet dose at transplantation ranged from 3500 – 13000 islets/kg b.w. One animal became overtly hyperglycemic (fasting glucose 18 mM) within 7 days after receiving 3500 islets/kg b.w. The other grafts (> 6000 islets/kg b.w.) were successful (fasting glucose <7mM) but demonstrated, compared to pre-operative values, a 50% reduced glucose clearance and insulin response at IVGTT, and a 90% reduced insulin secreting capacity at AT. Postprandially hyperglycemia (~ 10 mM) and in contrast to i.v. glucose and arginine, a normal insulin response was observed. The in vitro insulin response at 7.5 mM glucose to equimolar (0.1-1- 10 nM) concentrations of either CCK, GIP or both, demonstrated a sustained dose-response to GIP (up to 5x basal values) and a significant albeit small and transient effect of CCK as of 1 nM. No synergistic effect was observed.
In dogs ‘one-to-one’ transplantation was successful in 4/5 recipients of isolated islets. The difference in the effect of islet transplantation on the insulin response to intravenous glucose or – arginine and a meal, may be related to the postprandial, hyperglycemia enhanced, activation of the entero-insular axis, especially GIP.


Van der Burg MPM, Guicherit OR, Jansen JBMJ, Lamers CBHW, Scherft JP, Frölich M, Gooszen HG. Contribution of gut factors to canine isolated islet function (Abstract). Digestion 1990; 46 (Suppl 1): 14–15. https://doi.org/10.1159/000200356

PDF abstract shown below – file 20241009_1_Digestion.pdf

20241009_1_Digestion

Citation info : Greek Poster • Michel van der Burg • Miracles•Media • 20241009 • URL michelvanderburg.com/2024/10/09 | TakeNode 10afaddd-08b7-4dcd-ad7a-43228420ad59

Monitoring pancreatic islets in perifusion • 20240911

Perifusion system using the Acusyst-S in the Islet Transplant Lab started in 1987 in the University Hospital Leiden.

In this ‘perifusion’ device (6), the timeline of insulin secretion by the isolated islets is monitored in the laboratory.

Under the transparent cover, on the right side, hang 4 brown cylindrical plastic chambers (inside volume of 0.5 ml). In each of those chambers is the same portion of the isolated islets, with on average a total number of 453 islets, calculated as IEQ (4,5).

The test fluid – a simple salt solution containing glucose and, for example, gut hormones – flows via a small connecting tube from below into the chamber, where it flows around the islets (that’s why it’s called ‘peri’ fusion) and captures the sereted insulin, then exits the chamber from the top through an outlet tube into a cup in the rows of the collection cups on the rack on the right. Every 5 minutes, the outlet tube is manually moved to the next cup. Later, the amount of insulin in those cups is measured.

To the left of the block with the 4 chambers is the roller pump that pumps the various test fluids from the supply on the left in plastic tubes and bottles. The flow is switched using taps in those tubes.

Between the roller pump and the chambers, thin tubes run in loops over the black-metal heating block, which heats the fluids in the tubes and also keeps the entire space under the transparent cover at body temperature.

Incidentally, the walls of these thin tubes are gas-permeable, and the entire space under the cover is continuously ventilated with medical-grade air.

A maximum of 6 chambers can be used simultaneously, allowing up to 3 different tests to be conducted in parallel in duplicate.

Perifusion pilots had started in 1987 (1) in our Islet Transplant Lab of the University Hospital Leiden, and were followed in 1988, with large scale perifusion studies with isolated islets in our new project (more on this project here soon), in which the insulin secretion of isolated islets in perifusion experiments (in vitro) was compared with the function of the isolated islets after transplantation and also in concommitant pancreas transplantation experiments (in vivo), in support of the Leiden clinical pancreas transplantation program.

Some posters of this perifusion work have recently been posted online (2,3).

The scientific description of materials & methods of the perifusion work has been published in chapter 6 of the 1994 dissertation Pancreatic islet transplantation (4) and the similar paper (5).

Notes

1. Cradle of Islet Transplants • 20240829 • Michel van der Burg • michelvanderburg•com | URL https://michelvanderburg.com/2024/08/29/cradle-of-islet-transplants-20240829/

2. Gut factors controlling pancreatic islets • 20240910 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2024/09/10/gut-factors-controlling-pancreatic-islets-20240910/

3. Gut feelings of pancreatic islets • 20240906 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2024/09/06/gut-feelings-of-pancreatic-islets-20240906/

4. Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media | URL https://michelvanderburg.com/2022/11/25/pancreatic-islet-transplantation-thesis-repository-leiden-university-20221125/

5. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide, and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Peptides 1995; 60: 61–67.

6. Acusyst-S system (Endotronics, Coon Rapids, MO)

Citation info : Monitoring pancreatic islets in perifusion • 20240911 | Michel van der Burg | michelvanderburg•com | TakeNode fdf6f65c-28b7-4936-8c23-83225e6f2ff0

Gut factors controlling pancreatic islets • 20240910

Function after intrasplenic transplantation of islets of Langerhans in dogs.

The effect of gut hormones GLP-1 , GIP , and CCK-33 together with glucose on the insulin secretion capacity of isolated islets tested during storage of the islets in the laboratory (in vitro by perifusion), and after transplantation (in vivo).

Dutch poster (1) presented at the Surgeon Days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992.

An English edition of this presentation was presented in 1991, without the GLP-1 data (2) and posted again Sep 6, 2024 (3).

The poster was originally designed and produced on a Macintosh computer and Imagewriter dotmatrix printer, May 1992, using the MacDraw II application, with graphs from Cricket Graph. That 1992 Mac file was remastered today in LibreOffice Vanilla.

Notes

1. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Functie na intralienale transplantatie van eilandjes van Langerhans in de hond ( Function after intrasplenic transplantation of islets of Langerhans in dogs ). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992.

2. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

3. Gut feelings of pancreatic islets • 20240906 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2024/09/06/gut-feelings-of-pancreatic-islets-20240906/

4. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide, and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Peptides 1995; 60: 61–67.

5. Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media | URL https://michelvanderburg.com/2022/11/25/pancreatic-islet-transplantation-thesis-repository-leiden-university-20221125/

Citation info : Gut factors controlling pancreatic islets • 20240910 | Michel van der Burg | michelvanderburg•com | TakeNode 56ddc057-ffe9-4b70-bd33-180ea4b07877