Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • 20241203

Slides oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model. The pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.


Slideshow (pdf) oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241203_2 • TakeNode 5f53ae49-6fb8-4bef-a434-ac2ce8151c2a • File 1MEMO_20241203_2

Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999

Updates

Update 20260123 : Vimeo video replaced by YouTube copy

Citation info : Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • 1MEMO 20241203 • TakeNode ea1f1abd-6227-4ae6-985b-f8188310cd24 • URL michelvanderburg.com/2024/12/03/

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • 20241202

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202_1

I started an archive at Archive.org November 11, 2010 with this slideshow (pdf) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).

The presented results of this innovative islet purification method — developed over nearly 10 years (from 1989) in our Leiden islet laboratory (LUMC) — were the outcome of work I had done spring 1998 with the human islet transplantation group of Camillo Ricordi at the Diabetes Research Institute in Miami , Florida, USA.

Slideshow (pdf) Archive.org

Description

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999

Slides oral presentation Michel van der Burg, Aidspit meeting 25 Jan 1999, in Igls, Austria

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification. 18th Workshop Aidspit, Igls (Austria) Jan. 24-26, 1999

Program :
18TH WORKSHOP OF THE AIDSPIT STUDY GROUP – JANUARY 24-26, 1999 – IGLS/AUSTRIA . MONDAY, JAN 25, 1999 – KONGRESSHAUS IGLS, MAIN LECTURE HALL
SESSION 2 – ISLET ISOLATION AND TRANSPLANTATION
CHAIRMEN:K. FEDERLIN, GIESSEN, GERMANY – G. POZZA, MILAN, ITALY 9.00 – 10.30 FREE COMMUNICATIONS
9.45- Efficacy of the novel iodixanol-UWS density gradient for human islet purification.
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C; Leiden, The Netherlands and Miami, USA

File : mvdb19990118_1534_AIDSPIT.pdf | Uploaded 20101111 and replaced by the same pdf with updated metadata of the document on 20101112 (see Notes section).

Citation info (Archive file) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL https://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet • TakeNode 54cc8b66-672e-4b13-913c-449038ed0521

Video Slideshow

The slideshow video embedded below, was first posted at 1-memo.com in 2018 as Human Islet Purification Miami 1998 • @1MEMO 20181102

Notes

The slideshow pdf file is available for download at Archive.org. I advise against the other download options presented by Archive.org , which do not correctly represent the slideshow.

The file was also uploaded to Scribd at the same time – URL https://www.scribd.com/document/42197150/Efficacy-of-the-novel-iodixanol-UWS-density-gradient-for-human-islet-purification-michelvanderburg-19990125

Abstract

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Abstract). Acta Diabetol 1998; 35: 247. View or download abstract below .

Updates

20251101 – Vimeo video replaced by YouTube copy

20251101 – Added PDF copy abstract – file 1MEMO_20251102_1.pdf

Citation info (this post) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL michelvanderburg.com/2024/12/02/

Polygoon Journalist Jo Levi • 20241107

The unique 1935 sound film of the Amsterdam’s Great Synagogue Choir (1,2,3) was made by the popular Polygoon journalist Jo Levi.

Wondering who Jo Levi was , I searched online, and found this picture below (20241107_1) with Jo Levi.
Jo Levi is the second man from the left in this postwar photo of Polygoon men, posted by former Polygoon editor Fredo Krooshof at Cinecentrum Hilversum (4).


Polygoon Men • Miracles•Media • 20241107_1 | Source Cinecentrum Hilversum : Left to Right : Cees v.d. Wilden – Dir. Polygoon Haarlem; Jo Levi – Red. Pol.jnl; Philip Bloemendal – Red. and comm. Pol.jnl; Man in the background – name unknown; Gé Bresser – Procurator Polygoon; RVD man – name unknown (4).

Jo Levi together with Philip Bloemendal directed the Dutch cinema news for Polygoon. Philip Bloemendal , nicknamed “The voice of the Netherlands” , was for 40 years (from 1946 to 1986) the Dutch cinema newsreel announcer, voice-over, and editor . His voice-overs are The Voice of the postwar Dutch Polygoon news reels.

“Where they are not, nothing is happening” was the motto (5) of the cameramen of the Polygoon newsreel company in Holland.

May 1931 , two dutch newsreel companies, Polygoon in the city of Haarlem, and Profilti in The Hague, were competing to have the premiere of the first Dutch cinema newsreel with sound.
Below a May 17, 1931 Polygoon interview (6) with former cyclist and sportsman W.W. van der Vet on the occasion of his 85th birthday (20241107_2).

Polygoon News Reel May 17, 1931 • Miracles•Media • 20241107_2 • TakeNode 1967b92c-e090-40b3-84fb-d713aebddcc2

The interviewer, most likely Jo Levi, looks at the camera for a moment , captured in this film still shown below ( 20241107_3).


Polygoon May 17, 1931 Interview • Miracles•Media • 20241107_3 . TakeNode 9d87a032-0112-4e8d-822e-d805c5fdc168

Notes

1. Amsterdam’s Choir of the Great Synagogue 1935 • Michel van der Burg • Miracles•Media • 20190830 • URL https://michelvanderburg.com/2019/08/30/amsterdams-choir-of-the-great-synagogue-1935/

2. 1935 Sound Film Amsterdam’s Great Synagogue Choir • Michel van der Burg • Miracles•Media • 20241025 • URL https://michelvanderburg.com/2024/10/25/1935-sound-film-amsterdams-great-synagogue-choir-20241025/

3. 1935 Sound Film Amsterdam’s Great Synagogue Choir • Michel van der Burg • Miracles•Media • 20241025 • URL https://michelvanderburg.substack.com/p/1935-sound-film-amsterdams-great

4. Cinecentrum Hilversum. URL https://www.cinecentrum-hilversum.nl (Retrieved Oct. 25, 2024).

5. Polygoon (newsreel). Wikipedia (Retrieved 20241107). URL https://en.wikipedia.org/wiki/Polygoon_(newsreel)

6. Opa Vet (sportopa) voor de microfoon. Published 17 May 1931. Polygoon Hollands Nieuws (producer) / Nederlands Instituut voor Beeld en Geluid (Open Beelden archive)

Citation info : Polygoon Journalist Jo Levi • Michel van der Burg • Miracles•Media • 20241107

Wind in the Willows • Via #7 • 20241102

Poetry of motion…

‘Glorious, stirring sight!’ murmured Toad, never offering to move. ‘The poetry of motion! The real way to travel! The
only way to travel! Here to-day—in next week to-morrow!
Villages skipped, towns and cities jumped—always
somebody else’s horizon! O bliss! O poop-poop! O my! O
my!’*

Singing in the wind cycling on the willow lane in the Green Heart of Holland around noon, November 16, 2016.

Notes

* From The Wind in the Willows (1908) by Kenneth Grahame. Charles Scribner’s Sons (New York). Wikisource, Oct 31, 2024.

First edition posted that day as a 1 minute film at 1-memo•com (20161116) via Vimeo.

Citation info : Wind in the Willows • Via #7 • Michel van der Burg • Miracles•Media • @1MEMO 20241102 • TakeNode d50e4265-8a0c-4148-acc4-314a0880ebd5

1935 Sound Film Amsterdam’s Great Synagogue Choir • 20241025

Geluidsopname ter Groote Synagoge • NIW 15 Nov 1935 • Miracles•Media • 20241025

Friday, 15 Nov 1935 • NIW (1)

Sound recording at the Great Synagogue

The jubilee of the N.I.H.S., which has attracted interest in our community in many circles, has also attracted the attention of Polygoon, the well-known sound journalists. They wanted to record something specific of this jubilee and in honor of the jubilee on the sound film and insert it as part of the cinema newsreels this week.
Chief cantor I. E. Maroko and the synagogue choir, led by his well-known conductor S. H. Englander, were prepared to perform a synagogue song. And so the recording took place last Wednesday evening in the Great Synagogue. When we came to take a look at the Synagogue, which was already decorated for the occasion, at 8 o’clock, all preparations had been made. A number of lamps, together 25,000 candles strong, provided sufficient lighting. The choir sang Psalm 118, while Mr. Maroko sang the solos. The recording, which was made under the direction of the popular Polygoon man Jo Levi, and in which Max Tak was present, will certainly attract the interest of the cinema-loving public and for those who do not regularly go to the cinemas, we can certainly recommend a visit this time.”


This NIW 1935 news (1) describes the ‘sound film’ was made Wednesday 13 Nov 1935 under the direction of Polygoon man Jo Levi. Below the ‘sound film’ and first part of my description, as posted Aug 20, 2019 (2).

November 1935, on the occasion of the 300th anniversary of the NIHS Jewish Community of Amsterdam (orthodox Ashkenazi congregation started 1635) the dutch film factory Polygoon brought this unique cinema sound newsreel of the Amsterdam Choir of the Great Synagogue led by choirmaster Samuel Henri (Sam) Englander, with a solo perfomance by chazzan (cantor) Izrael Eljasz Maroko in the Great Synagogue (inauguration building 1671) – now home to the Jewish Historical Museum in Amsterdam, Holland (2).

Notes

1. Today I found this article on the Polygoon ‘sound recording’ (2) in the dutch weekly ‘Nieuw Israelietisch weekblad’ (Transl: New Israelite Weekly) of Friday, November 15, 1935. URL Delpher.nl (retrieved 20241025) .

Dutch transcript

Geluidsopname ter Groote Synagoge

Het jubileum der N.I.H.S., dat de belangstelling in vele kringen op onze gemeente heeft gevestigd, heeft ook de aandacht getrokken van Polygoon, de bekende geluidsjournalisten. Men wilde n.l. iets specifieks van dit jubileum en ter eere van het jubileum op de geluidsfilm vastleggen en dit deze week als onderdeel in de bioscoopjournaals inlasschen.
Oppervoorzanger I. E. Maroko en het sjoelkoor, onder leiding van zijn bekenden dirigent S. H. Englander, werden bereid gevonden om een synagogaal gezang ten gehoore te brengen. En zoo vond Woensdagavond j.I. de opname plaats in de Groote Synagoge. Toen wij om 8 uur een kijkje kwamen nemen in de reeds in feesttooi gestoken Synagoge, waren alle toebereidselen getroffen. Een aantal lampen, tezamen 25.000 kaarsen sterk, zorgde voor voldoende belichting.
Het koor zong Psalm 118, terwijl de heer Maroko de soli zong. De opname, welke onder leiding van den populairen Polygoonman Jo Levi geschiedde, werd vervaardigd en waarbij o.a. Max Tak tegenwoordig was, zal zeer zeker de belangstelling van het bioscoopminnend publiek trekken en zij, die niet geregeld in de cinema’s komen, kunnen wij ditmaal zeker een gang daarheen aanbevelen.

2. Amsterdam’s Choir of the Great Synagogue 1935 • Michel van der Burg • Miracles•Media • 20190830 • URL https://michelvanderburg.com/2019/08/30/amsterdams-choir-of-the-great-synagogue-1935/

Citation info : 1935 Sound Film Amsterdam’s Great Synagogue Choir • Michel van der Burg • Miracles•Media • 20241025

ADD Diabetes Group 1989, St Edmund Hall, Oxford • 20241019

St Edmund Hall, University of Oxford, July 1989 • Michel van der Burg • Miracles•Media • 20241019_1 • TakeNode af426345-4fb3-48a3-8d5e-4368c56826aa

Venue of the Seventh Annual Meeting of the Anglo–Danish–Dutch Diabetes Group*, Oxford (UK) July 5–9, 1989.

The first international meeting were our new concept was presented, of pancreatic islet isolation in the University of Wisconsin Solution (UW-Solution or UWS) a solution designed recently for preservation of a donor pancreas during cold storage prior to transplantation.

Quote :


“…A few months ago, we adopted an entirely new approach to islet isolation.
Recently a new cold storage organ preservation solution was developed at the University of Wisconsin, the UW solution, allowing long term, 24 to 72 h, cold storage of the canine pancreas.
Since islet isolation is largely performed in the cold, we tested this preservation solution as the isolation medium throughout the isolation procedure. This slide shows that using the UW solution, purity has increased to over 90%…”

In addition our first successful pre-clinical transplant, we had performed the last month, was reported as breaking news at the end of the talk.

Quote :

“Three weeks ago we did our first autotransplantation of isolated islets from the whole gland by retrograde infusion into the spleen. The dog is doing fine: fasting glucose amounts to 5-6 mM and postprandial glucose between 6-8 mM ; postprandial insulin increases 6-7 fold. Thank you.”


St Edmund Hall, Oxford, 1989, 7th Meeting ADDD Group • Michel van der Burg • Miracles•Media • 20241019_2 • TakeNode ca40adf0-3b86-4ada-93a8-126c6768d420

Notes


Seventh Annual Meeting Anglo Danish Dutch Diabetes Group • Michel van der Burg • Miracles•Media • 20241019_3 • TakeNode bffdea47-9464-4f7b-8299-ec299d41ca4d

* Group supported through the Nordisk UK Professional Programme.

Van der Burg MPM, Gooszen HG, Scherft JP, Terpstra JL, Frölich M, Guicherit OR, Lemkes HHPJ, Bruijn JA. The isolation of canine pancreatic islets for transplantation. Seventh Annual Meeting Anglo Danish Dutch Diabetes Group, Oxford (UK) July 5–9, 1989.

Van der Burg MPM, Gooszen HG, Guicherit OR, Jansen JBMJ, Frölich M, Lamers CBHW. The mechanism of pancreatic duct obliteration induced hyperglycemia. Seventh Annual Meeting Anglo Danish Dutch Diabetes Group, Oxford (UK) July 5–9, 1989.

Citation info : ADD Diabetes Group 1989, St Edmund Hall, Oxford • Michel van der Burg • Miracles•Media • 20241019 • URL michelvanderburg•com

Review RESCUE RUN • 20240927

RESCUE RUN : Capt. Jake Rogers’ Daring Return to Occupied Europe • John Winn Miller

After escaping the treacherous waters of WWII, Captain Jake Rogers leads his crew on a daring mission across Nazi-occupied Europe to rescue the father of his beloved, entangled in a web of espionage, betrayal, and relentless pursuit.

Review of John Winn Miller’s novel ‘Rescue Run’

John Winn Miller, a veteran of investigative journalism for decades, masterfully weaves historical detail into his World War II novel Rescue Run. The story follows American ship’s captain Jake Rogers, who, after his U.S. Liberty ship carrying war supplies is wrecked in the North Atlantic, first lands in Ireland. He then sets out with a few of his loyal men on a perilous mission to rescue the father of Miriam Maduro, the love of his life, from the Westerbork transit camp in Nazi-occupied Holland.

A gruelling journey follows from Amsterdam across Nazi-occupied Europe to Spain – on foot, by train, and by boat – via a long series of hiding places, historical locations and events, aided by well-known and lesser-known resistance organisations and historical figures, besieged by con men, double agents, gangsters and pursued by a ruthless Dutch bounty hunter.

As a non-native English speaker, I initially struggled a bit with the first few chapters, particularly the maritime terms and rich language used in the sea adventure with the Liberty ship, besides the introduction of the many characters. However, once past those pages, the novel became a true page-turner. The gripping, almost cinematic narrative had me finishing the book in just two or three days, despite also spending some time online searching for even more historical context, for example when Rogers is helped by the Dutch resistance group ‘Groep 2000’ led by Jacoba van Tongeren, and when characters like Etty Hillesum and Audrey Hepburn appear in the story.

Blending Fiction and Nonfiction

I rarely read fiction these days — almost exclusively occupied with non-fiction — but John Miller’s work intrigued me. I was curious to see how he managed to incorporate the reality of Nazi-occupied Europe, and in particular the Westerbork transit camp, into fiction. The result is exceptional. The blend of fiction and nonfiction strengthens the narrative, bringing the past vividly to life. Miller also provides an extensive set of notes at the end of the book, offering in-depth background information on the events and historical figures featured in the story, detailing also what happened to them after the events of the novel.

I especially appreciate how John Winn Miller brings the wartime past to life in Rescue Run, with accuracy and rich detail, from multiple perspectives. His cinematic storytelling draws readers into a narrative that inspires further exploration of this history.

Michel van der Burg, filmmaker, editor of Settela•Com


Notes

Additional Information:

  • Title: Rescue Run: Capt. Jake Rogers’ Daring Return to Occupied Europe
  • Author: John Winn Miller
  • Publisher: Bancroft Press
  • ISBN: 9781610886437 (HC), 9781610886451 (Ebook), 9781610886475 (Audiobook)
  • Release Date: Expected March 4, 2025
  • URL: Bancroft Press – Rescue Run

John Winn Miller is an award-winning investigative reporter, foreign correspondent, editor, publisher, screenwriter, indie movie producer and novelist. | Photo Bancroft Press. | More info at Miller’s website URL https://www.johnwinnmiller.com

John Winn Miller


Though Rescue Run is a sequel to Miller’s first novel, The Hunt for the Peggy C, no prior knowledge of the first book is required. A summary of the prequel is included for new readers or those needing a refresher.

Westerbork Film Frame

This review was prompted by my contribution of a still (image below) from the Westerbork film for Rescue Run’s jacket/cover, in collaboration with designer Christine Van Bree , and author John Winn Miller.
John kindly provided me with a link for a free download of the Advance Reader Copy on the BookSirens’ platform. Since I’m documenting the Westerbork film through the online magazine Settela•Com, I happily accepted BookSiren’s invitation to join the review team.


Deportation | 20240225 | Settela•Com | Commander Albert Gemmeker oversees the deportation of Jews, Sinti, and Roma from the Westerbork transit camp May 19, 1944 | Frame 7426 from Deportation Westerbork Film | 20210719 | Settela•Com | ISSN 2949 9313
Citation info : Review RESCUE RUN • 20240927 • Michel van der Burg • Settela•Com • ISSN 2949-9313