Category: image

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • 20241202

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202_1

I started an archive at Archive.org November 11, 2010 with this slideshow (pdf) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).

The presented results of this innovative islet purification method — developed over nearly 10 years (from 1989) in our Leiden islet laboratory (LUMC) — were the outcome of work I had done spring 1998 with the human islet transplantation group of Camillo Ricordi at the Diabetes Research Institute in Miami , Florida, USA.

Slideshow (pdf) Archive.org

Description

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999

Slides oral presentation Michel van der Burg, Aidspit meeting 25 Jan 1999, in Igls, Austria

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification. 18th Workshop Aidspit, Igls (Austria) Jan. 24-26, 1999

Program :
18TH WORKSHOP OF THE AIDSPIT STUDY GROUP – JANUARY 24-26, 1999 – IGLS/AUSTRIA . MONDAY, JAN 25, 1999 – KONGRESSHAUS IGLS, MAIN LECTURE HALL
SESSION 2 – ISLET ISOLATION AND TRANSPLANTATION
CHAIRMEN:K. FEDERLIN, GIESSEN, GERMANY – G. POZZA, MILAN, ITALY 9.00 – 10.30 FREE COMMUNICATIONS
9.45- Efficacy of the novel iodixanol-UWS density gradient for human islet purification.
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C; Leiden, The Netherlands and Miami, USA

File : mvdb19990118_1534_AIDSPIT.pdf | Uploaded 20101111 and replaced by the same pdf with updated metadata of the document on 20101112 (see Notes section).

Citation info (Archive file) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL https://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet • TakeNode 54cc8b66-672e-4b13-913c-449038ed0521

Video Slideshow

The slideshow video embedded below, was first posted at 1-memo.com in 2018 as Human Islet Purification Miami 1998 • @1MEMO 20181102

Notes

The slideshow pdf file is available for download at Archive.org. I advise against the other download options presented by Archive.org , which do not correctly represent the slideshow.

The file was also uploaded to Scribd at the same time – URL https://www.scribd.com/document/42197150/Efficacy-of-the-novel-iodixanol-UWS-density-gradient-for-human-islet-purification-michelvanderburg-19990125

Abstract

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Abstract). Acta Diabetol 1998; 35: 247. View or download abstract below .

1MEMO_20251102_1Download

Updates

20251101 – Vimeo video replaced by YouTube copy

20251101 – Added PDF copy abstract – file 1MEMO_20251102_1.pdf

Citation info (this post) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL michelvanderburg.com/2024/12/02/

Polygoon Journalist Jo Levi • 20241107

The unique 1935 sound film of the Amsterdam’s Great Synagogue Choir (1,2,3) was made by the popular Polygoon journalist Jo Levi.

Wondering who Jo Levi was , I searched online, and found this picture below (20241107_1) with Jo Levi.
Jo Levi is the second man from the left in this postwar photo of Polygoon men, posted by former Polygoon editor Fredo Krooshof at Cinecentrum Hilversum (4).


Polygoon Men • Miracles•Media • 20241107_1 | Source Cinecentrum Hilversum : Left to Right : Cees v.d. Wilden – Dir. Polygoon Haarlem; Jo Levi – Red. Pol.jnl; Philip Bloemendal – Red. and comm. Pol.jnl; Man in the background – name unknown; Gé Bresser – Procurator Polygoon; RVD man – name unknown (4).

Jo Levi together with Philip Bloemendal directed the Dutch cinema news for Polygoon. Philip Bloemendal , nicknamed “The voice of the Netherlands” , was for 40 years (from 1946 to 1986) the Dutch cinema newsreel announcer, voice-over, and editor . His voice-overs are The Voice of the postwar Dutch Polygoon news reels.

“Where they are not, nothing is happening” was the motto (5) of the cameramen of the Polygoon newsreel company in Holland.

May 1931 , two dutch newsreel companies, Polygoon in the city of Haarlem, and Profilti in The Hague, were competing to have the premiere of the first Dutch cinema newsreel with sound.
Below a May 17, 1931 Polygoon interview (6) with former cyclist and sportsman W.W. van der Vet on the occasion of his 85th birthday (20241107_2).

Polygoon News Reel May 17, 1931 • Miracles•Media • 20241107_2 • TakeNode 1967b92c-e090-40b3-84fb-d713aebddcc2

The interviewer, most likely Jo Levi, looks at the camera for a moment , captured in this film still shown below ( 20241107_3).


Polygoon May 17, 1931 Interview • Miracles•Media • 20241107_3 . TakeNode 9d87a032-0112-4e8d-822e-d805c5fdc168

Notes

1. Amsterdam’s Choir of the Great Synagogue 1935 • Michel van der Burg • Miracles•Media • 20190830 • URL https://michelvanderburg.com/2019/08/30/amsterdams-choir-of-the-great-synagogue-1935/

2. 1935 Sound Film Amsterdam’s Great Synagogue Choir • Michel van der Burg • Miracles•Media • 20241025 • URL https://michelvanderburg.com/2024/10/25/1935-sound-film-amsterdams-great-synagogue-choir-20241025/

3. 1935 Sound Film Amsterdam’s Great Synagogue Choir • Michel van der Burg • Miracles•Media • 20241025 • URL https://michelvanderburg.substack.com/p/1935-sound-film-amsterdams-great

4. Cinecentrum Hilversum. URL https://www.cinecentrum-hilversum.nl (Retrieved Oct. 25, 2024).

5. Polygoon (newsreel). Wikipedia (Retrieved 20241107). URL https://en.wikipedia.org/wiki/Polygoon_(newsreel)

6. Opa Vet (sportopa) voor de microfoon. Published 17 May 1931. Polygoon Hollands Nieuws (producer) / Nederlands Instituut voor Beeld en Geluid (Open Beelden archive)

Citation info : Polygoon Journalist Jo Levi • Michel van der Burg • Miracles•Media • 20241107

ADD Diabetes Group 1989, St Edmund Hall, Oxford • 20241019

St Edmund Hall, University of Oxford, July 1989 • Michel van der Burg • Miracles•Media • 20241019_1 • TakeNode af426345-4fb3-48a3-8d5e-4368c56826aa

Venue of the Seventh Annual Meeting of the Anglo–Danish–Dutch Diabetes Group*, Oxford (UK) July 5–9, 1989.

The first international meeting were our new concept was presented, of pancreatic islet isolation in the University of Wisconsin Solution (UW-Solution or UWS) a solution designed recently for preservation of a donor pancreas during cold storage prior to transplantation.

Quote :


“…A few months ago, we adopted an entirely new approach to islet isolation.
Recently a new cold storage organ preservation solution was developed at the University of Wisconsin, the UW solution, allowing long term, 24 to 72 h, cold storage of the canine pancreas.
Since islet isolation is largely performed in the cold, we tested this preservation solution as the isolation medium throughout the isolation procedure. This slide shows that using the UW solution, purity has increased to over 90%…”

In addition our first successful pre-clinical transplant, we had performed the last month, was reported as breaking news at the end of the talk.

Quote :

“Three weeks ago we did our first autotransplantation of isolated islets from the whole gland by retrograde infusion into the spleen. The dog is doing fine: fasting glucose amounts to 5-6 mM and postprandial glucose between 6-8 mM ; postprandial insulin increases 6-7 fold. Thank you.”


St Edmund Hall, Oxford, 1989, 7th Meeting ADDD Group • Michel van der Burg • Miracles•Media • 20241019_2 • TakeNode ca40adf0-3b86-4ada-93a8-126c6768d420

Notes


Seventh Annual Meeting Anglo Danish Dutch Diabetes Group • Michel van der Burg • Miracles•Media • 20241019_3 • TakeNode bffdea47-9464-4f7b-8299-ec299d41ca4d

* Group supported through the Nordisk UK Professional Programme.

Van der Burg MPM, Gooszen HG, Scherft JP, Terpstra JL, Frölich M, Guicherit OR, Lemkes HHPJ, Bruijn JA. The isolation of canine pancreatic islets for transplantation. Seventh Annual Meeting Anglo Danish Dutch Diabetes Group, Oxford (UK) July 5–9, 1989.

Van der Burg MPM, Gooszen HG, Guicherit OR, Jansen JBMJ, Frölich M, Lamers CBHW. The mechanism of pancreatic duct obliteration induced hyperglycemia. Seventh Annual Meeting Anglo Danish Dutch Diabetes Group, Oxford (UK) July 5–9, 1989.

Citation info : ADD Diabetes Group 1989, St Edmund Hall, Oxford • Michel van der Burg • Miracles•Media • 20241019 • URL michelvanderburg•com

ZX81 • 20241018

Sinclair’s ZX81 computer (1KB on-board memory , with a 64K memory expansion module inserted at the back) – my first PC – that I started using from 1983 for automation of work in our experimental pancreas and islet transplantation research project – for creating (‘coding’) and using the entire suit of apps, needed for the raw data processing and the (statistical and pharmacokinetic) analysis.
The hardware set-up included a TV (B/W) as monitor, (mono audio) tape recorder for data storage (apps), and a small thermal printer.

Citation info : ZX81 • Michel van der Burg • Miracles•Media • 20241018 • TakeNode 9c595df8-8091-497c-9c9c-ff85fe4de397 • URL michelvanderburg•com

Radical Right Front • 20241011

Miracles•Media • 20241011_1 • Harzburger Front founding field service with preacher Bruno Doehring on the Kaltental-Wiese (meadow) in Bad Harzburg, October 11, 1931.

Founding of the ‘Harzburger Front’ in Bad Harzburg, October 11, 1931 – an alliance between right-wing, nationalist, conservative elements and Hitler, to attempt to topple the Weimar government of chancellor Heinrich Brüning. The field service on the Kaltental-Wiese (meadow) in Bad Harzburg, with the Berlin Protestant preacher Bruno Doehring, on the platform (pulpit).


Miracles•Media • 20241011 2 • Nazi Party at the founding of the ‘Harzburger Front’ in Bad Harzburg, October 11, 1931. Bundesarchiv image 102 02134 • CC BY SA 3.0

The Nazi Party delegation during the field service on the Kaltental-Wiese (meadow) in Bad Harzburg. First row , left to right:
Gerret Korsemann (SA-leader), Heinrich Himmler (SS Chief), Ernst Röhm (SA Chief), Franz Ritter von Hörauf, Hermann Göring (Reichsmarschall, Commander-in-Chief of the Luftwaffe, Prime Minister of Prussia), Bernhard Rust (Prussian Minister of Culture).

Notes

Last update 20241011-2228

Update 20241011-2228 : Facebook does not allow me to share todays post with images from my website michelvanderburg•com or michelvanderburg.substack (posts are removed)

Citation info : Radical Right Front • Miracles•Media • 20241011 • URL michelvanderburg.com/2024/10/11/

Press Release • 20241010

LUMC, 11 June 2003, Leiden, Holland • Michel van der Burg • Miracles•Media • 20241010_1 • TakeNode 2d46097b-ca09-442d-9cf5-fc347a27fc46 | Image shot using the Konica Revio C2 – my first digital camera.

While searching my archive today, I encountered the Press release Sep 27, 2001 of the dutch National Health Care Institute (CVZ), advicing the Ministry of Health, Welfare and Sport (VWS) to grant permission for Transplantation of Islets of Langerhans in the Leiden University Medical Center (LUMC).

That press release is included now (in note 6) in the recent post on the Leiden Islet Laboratory History at the site – https://michelvanderburg.com/2024/08/03/leiden-islet-laboratory-history-20240803/

Citation info : Press Release • Michel van der Burg • Miracles•Media • 20241010 • URL michelvanderburg.com/2024/10/10/

Greek Poster • 20241009

Greek Poster • Michel van der Burg • Miracles•Media • 20241009

Funny. LibreOffice Vanilla Issue.

Greek characters appeared in this poster originally designed with english text and produced on a Macintosh computer and Imagewriter dotmatrix printer, September 3, 1990, using the MacDraw II application, with graphs from Cricket Graph.
That 1990 Mac file was remastered today in LibreOffice Vanilla (other minor issues in graphs not corrected). Generally LibreOffice does a great job with these ancient files. I recently posted several of such posters at site michelvanderburg.com.
However, some of the posters retrieved from my archive show up with Greek characters when opened in LibreOffice, like this poster produced in English (with roman characters).
Those problematic posters have in common that the Apple Chicago font was used – the interface font in Apple’s early versions of Mac OS from 1984 to 1997. Clearly LibreOffice cannot handle this Chicago font correctly. I have not found a solution for this.
Text in other posters, produced with Helvetica, had only minor issues in LibreOffice, Helvetica italics text appeared as regular Times.

The original poster and abstract (below) were presented at the 8th International Symposium on Gastrointestinal Hormones, in Timmendorfer Strand Baltic Sea (FRG) September 4–8, 1990.

Abstract

Contribution of gut factors to canine isolated islet function
M.P.M. van der Burg, O.R. Guicherit, J.B.M.J. Jansen, C.B.H.W. Lamers, J.P. Scherft, M. Frölich, H.G. Gooszen.
Departments of Surgery, Gastroenterology. Endocrinology and Cel Biology. University Hospital, Leiden, The Netherlands.
Techniques for isolating islets of Langerhans from the large mammalian pancreas have improved to the point where islet transplantation as a therapeutical approach to human diabetes has become more realistic. The quality of metabolic control is however largely unknown. We studied canine isolated-islet function both in vivo after autotransplantation, as well as in vitro by perifusion.
Five normal dogs underwent total pancreatectomy. Islets were isolated from the excised pancreas by collagenase digestion, dispersion and purification with filtration and density gradients. Isolated islets were autotransplanted into the spleen of the dog by retrograde venous infusion. Graft function was assessed up to 3 mo by determining the glucose and insulin response to an intravenous glucose injection (IVGTT), i.v. arginine injection during 35 mM glucose clamp (AT), and a meal. In addition (n = 4) the in vitro insulin response of overnight cultured canine islets was studied by perifusion with cholecystokinin (CCK-33) and glucose-dependent insulinotropic polypeptide (GIP).
The islet dose at transplantation ranged from 3500 – 13000 islets/kg b.w. One animal became overtly hyperglycemic (fasting glucose 18 mM) within 7 days after receiving 3500 islets/kg b.w. The other grafts (> 6000 islets/kg b.w.) were successful (fasting glucose <7mM) but demonstrated, compared to pre-operative values, a 50% reduced glucose clearance and insulin response at IVGTT, and a 90% reduced insulin secreting capacity at AT. Postprandially hyperglycemia (~ 10 mM) and in contrast to i.v. glucose and arginine, a normal insulin response was observed. The in vitro insulin response at 7.5 mM glucose to equimolar (0.1-1- 10 nM) concentrations of either CCK, GIP or both, demonstrated a sustained dose-response to GIP (up to 5x basal values) and a significant albeit small and transient effect of CCK as of 1 nM. No synergistic effect was observed.
In dogs ‘one-to-one’ transplantation was successful in 4/5 recipients of isolated islets. The difference in the effect of islet transplantation on the insulin response to intravenous glucose or – arginine and a meal, may be related to the postprandial, hyperglycemia enhanced, activation of the entero-insular axis, especially GIP.


Van der Burg MPM, Guicherit OR, Jansen JBMJ, Lamers CBHW, Scherft JP, Frölich M, Gooszen HG. Contribution of gut factors to canine isolated islet function (Abstract). Digestion 1990; 46 (Suppl 1): 14–15. https://doi.org/10.1159/000200356

PDF abstract shown below – file 20241009_1_Digestion.pdf

20241009_1_Digestion

Citation info : Greek Poster • Michel van der Burg • Miracles•Media • 20241009 • URL michelvanderburg.com/2024/10/09 | TakeNode 10afaddd-08b7-4dcd-ad7a-43228420ad59