Slides oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia
A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model. The pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.
Slideshow (pdf) oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia
Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241203_2 • TakeNode 5f53ae49-6fb8-4bef-a434-ac2ce8151c2a • File 1MEMO_20241203_2
Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999
Updates
Update 20260123 : Vimeo video replaced by YouTube copy
Citation info : Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • 1MEMO 20241203 • TakeNode ea1f1abd-6227-4ae6-985b-f8188310cd24 • URL michelvanderburg.com/2024/12/03/
Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202_1
I started an archive at Archive.org November 11, 2010 with this slideshow (pdf) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).
The presented results of this innovative islet purification method — developed over nearly 10 years (from 1989) in our Leiden islet laboratory (LUMC) — were the outcome of work I had done spring 1998 with the human islet transplantation group of Camillo Ricordi at the Diabetes Research Institute in Miami , Florida, USA.
Slideshow (pdf) Archive.org
Description
Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999
Slides oral presentation Michel van der Burg, Aidspit meeting 25 Jan 1999, in Igls, Austria
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification. 18th Workshop Aidspit, Igls (Austria) Jan. 24-26, 1999
Program : 18TH WORKSHOP OF THE AIDSPIT STUDY GROUP – JANUARY 24-26, 1999 – IGLS/AUSTRIA . MONDAY, JAN 25, 1999 – KONGRESSHAUS IGLS, MAIN LECTURE HALL SESSION 2 – ISLET ISOLATION AND TRANSPLANTATION CHAIRMEN:K. FEDERLIN, GIESSEN, GERMANY – G. POZZA, MILAN, ITALY 9.00 – 10.30 FREE COMMUNICATIONS 9.45- Efficacy of the novel iodixanol-UWS density gradient for human islet purification. Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C; Leiden, The Netherlands and Miami, USA
File : mvdb19990118_1534_AIDSPIT.pdf | Uploaded 20101111 and replaced by the same pdf with updated metadata of the document on 20101112 (see Notes section).
The slideshow video embedded below, was first posted at 1-memo.com in 2018 as Human Islet Purification Miami 1998 • @1MEMO 20181102
Notes
The slideshow pdf file is available for download at Archive.org. I advise against the other download options presented by Archive.org , which do not correctly represent the slideshow.
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Abstract). Acta Diabetol 1998; 35: 247. View or download abstract below .
20251101 – Added PDF copy abstract – file 1MEMO_20251102_1.pdf
Citation info (this post) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL michelvanderburg.com/2024/12/02/
The unique 1935 sound film of the Amsterdam’s Great Synagogue Choir (1,2,3) was made by the popular Polygoon journalist Jo Levi.
Wondering who Jo Levi was , I searched online, and found this picture below (20241107_1) with Jo Levi. Jo Levi is the second man from the left in this postwar photo of Polygoon men, posted by former Polygoon editor Fredo Krooshof at Cinecentrum Hilversum (4).
Polygoon Men • Miracles•Media • 20241107_1 | Source Cinecentrum Hilversum : Left to Right : Cees v.d. Wilden – Dir. Polygoon Haarlem; Jo Levi – Red. Pol.jnl; Philip Bloemendal – Red. and comm. Pol.jnl; Man in the background – name unknown; Gé Bresser – Procurator Polygoon; RVD man – name unknown (4).
Jo Levi together with Philip Bloemendal directed the Dutch cinema news for Polygoon. Philip Bloemendal , nicknamed “The voice of the Netherlands” , was for 40 years (from 1946 to 1986) the Dutch cinema newsreel announcer, voice-over, and editor . His voice-overs are The Voice of the postwar Dutch Polygoon news reels.
“Where they are not, nothing is happening” was the motto (5) of the cameramen of the Polygoon newsreel company in Holland.
May 1931 , two dutch newsreel companies, Polygoon in the city of Haarlem, and Profilti in The Hague, were competing to have the premiere of the first Dutch cinema newsreel with sound. Below a May 17, 1931 Polygoon interview (6) with former cyclist and sportsman W.W. van der Vet on the occasion of his 85th birthday (20241107_2).
6. Opa Vet (sportopa) voor de microfoon. Published 17 May 1931. Polygoon Hollands Nieuws (producer) / Nederlands Instituut voor Beeld en Geluid (Open Beelden archive)
Citation info : Polygoon Journalist Jo Levi • Michel van der Burg • Miracles•Media • 20241107
St Edmund Hall, University of Oxford, July 1989 • Michel van der Burg • Miracles•Media • 20241019_1 • TakeNode af426345-4fb3-48a3-8d5e-4368c56826aa
Venue of the Seventh Annual Meeting of the Anglo–Danish–Dutch Diabetes Group*, Oxford (UK) July 5–9, 1989.
The first international meeting were our new concept was presented, of pancreatic islet isolation in the University of Wisconsin Solution (UW-Solution or UWS) a solution designed recently for preservation of a donor pancreas during cold storage prior to transplantation.
Quote :
“…A few months ago, we adopted an entirely new approach to islet isolation. Recently a new cold storage organ preservation solution was developed at the University of Wisconsin, the UW solution, allowing long term, 24 to 72 h, cold storage of the canine pancreas. Since islet isolation is largely performed in the cold, we tested this preservation solution as the isolation medium throughout the isolation procedure. This slide shows that using the UW solution, purity has increased to over 90%…”
In addition our first successful pre-clinical transplant, we had performed the last month, was reported as breaking news at the end of the talk.
Quote :
“Three weeks ago we did our first autotransplantation of isolated islets from the whole gland by retrograde infusion into the spleen. The dog is doing fine: fasting glucose amounts to 5-6 mM and postprandial glucose between 6-8 mM ; postprandial insulin increases 6-7 fold. Thank you.”
St Edmund Hall, Oxford, 1989, 7th Meeting ADDD Group • Michel van der Burg • Miracles•Media • 20241019_2 • TakeNode ca40adf0-3b86-4ada-93a8-126c6768d420
Notes
Seventh Annual Meeting Anglo Danish Dutch Diabetes Group • Michel van der Burg • Miracles•Media • 20241019_3 • TakeNode bffdea47-9464-4f7b-8299-ec299d41ca4d
* Group supported through the Nordisk UK Professional Programme.
Van der Burg MPM, Gooszen HG, Scherft JP, Terpstra JL, Frölich M, Guicherit OR, Lemkes HHPJ, Bruijn JA. The isolation of canine pancreatic islets for transplantation. Seventh Annual Meeting Anglo Danish Dutch Diabetes Group, Oxford (UK) July 5–9, 1989.
Van der Burg MPM, Gooszen HG, Guicherit OR, Jansen JBMJ, Frölich M, Lamers CBHW. The mechanism of pancreatic duct obliteration induced hyperglycemia. Seventh Annual Meeting Anglo Danish Dutch Diabetes Group, Oxford (UK) July 5–9, 1989.
Citation info : ADD Diabetes Group 1989, St Edmund Hall, Oxford • Michel van der Burg • Miracles•Media • 20241019 • URL michelvanderburg•com
Sinclair’s ZX81 computer (1KB on-board memory , with a 64K memory expansion module inserted at the back) – my first PC – that I started using from 1983 for automation of work in our experimental pancreas and islet transplantation research project – for creating (‘coding’) and using the entire suit of apps, needed for the raw data processing and the (statistical and pharmacokinetic) analysis.
The hardware set-up included a TV (B/W) as monitor, (mono audio) tape recorder for data storage (apps), and a small thermal printer.
Citation info : ZX81 • Michel van der Burg • Miracles•Media • 20241018 • TakeNode 9c595df8-8091-497c-9c9c-ff85fe4de397 • URL michelvanderburg•com
Miracles•Media • 20241011_1 • Harzburger Front founding field service with preacher Bruno Doehring on the Kaltental-Wiese (meadow) in Bad Harzburg, October 11, 1931.
Founding of the ‘Harzburger Front’ in Bad Harzburg, October 11, 1931 – an alliance between right-wing, nationalist, conservative elements and Hitler, to attempt to topple the Weimar government of chancellor Heinrich Brüning. The field service on the Kaltental-Wiese (meadow) in Bad Harzburg, with the Berlin Protestant preacher Bruno Doehring, on the platform (pulpit).
Miracles•Media • 20241011 2 • Nazi Party at the founding of the ‘Harzburger Front’ in Bad Harzburg, October 11, 1931. Bundesarchiv image 102 02134 • CC BY SA 3.0
The Nazi Party delegation during the field service on the Kaltental-Wiese (meadow) in Bad Harzburg. First row , left to right: Gerret Korsemann (SA-leader), Heinrich Himmler (SS Chief), Ernst Röhm (SA Chief), Franz Ritter von Hörauf, Hermann Göring (Reichsmarschall, Commander-in-Chief of the Luftwaffe, Prime Minister of Prussia), Bernhard Rust (Prussian Minister of Culture).
Notes
Last update 20241011-2228
Update 20241011-2228 : Facebook does not allow me to share todays post with images from my website michelvanderburg•com or michelvanderburg.substack (posts are removed)
Citation info : Radical Right Front • Miracles•Media • 20241011 • URL michelvanderburg.com/2024/10/11/
LUMC, 11 June 2003, Leiden, Holland • Michel van der Burg • Miracles•Media • 20241010_1 • TakeNode 2d46097b-ca09-442d-9cf5-fc347a27fc46 | Image shot using the Konica Revio C2 – my first digital camera.
While searching my archive today, I encountered the Press release Sep 27, 2001 of the dutch National Health Care Institute (CVZ), advicing the Ministry of Health, Welfare and Sport (VWS) to grant permission for Transplantation of Islets of Langerhans in the Leiden University Medical Center (LUMC).
