Author: michelvanderburg

PentaStarch UWS Pancreatic Islet Purification • 20240802

Following first introduction of the use UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options.
One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS (7) . Note that Pentastarch is a component of UWS.

The Percoll-UWS poster was posted recently (8)
Here the remastered poster presentation on Pentastarch-UWS :
Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 3 strips) were then glued on site along the perforation, creating the 36/24 inch poster.
Finally the photo print of the purified islets was pasted in the poster at the spot at the venue in Lyon. In Lyon a photo of a dithizone stained Pentastarch purified islet sample was shown. To save time the archive was not searched for that image, and another image of Pentastarch purified islets was inserted in this remastered edition, a reflection contrast micrograph of an ultrathin section of Pentastarch purified islets immuno-stained with gold on insulin in the islet cells.

Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla and now learned to use the app to further correct most conversion issues. Pixelmator Pro was used for additional ‘repair’ and insertion of the photo scan.

Notes

1. Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2022/08/22/minneapolis-1989-poster-uw-solution-islet-isolation-20220822/

2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

6. Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media | URL https://michelvanderburg.com/2022/11/25/pancreatic-islet-transplantation-thesis-repository-leiden-university-20221125/

7. Pentastarch is the synthetic colloid component of UWS (ViaSpan / Belzer solution), a purified fraction (250 kD) of hydroxyethyl starch. Pentastarch powder was used here to increase the Pentastarch concentration and density of the UWS.

8. Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com

Citation info : PentaStarch UWS Pancreatic Islet Purification • 20240802 | Michel van der Burg | michelvanderburg•com | TakeNode d11f1cef-a343-4466-8814-3e52a0261cae

Percoll UWS Pancreatic Islet Purification • 20240731

Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e

Following first introduction of the use of UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options.
One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS . Note that Pentastarch is a component of UWS.

Here one of these poster presentations, on Percoll-UWS :
Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application, inserted with a bar graph prepared in the Cricket Graph app.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 5 strips) were then glued on site along the perforation, creating a 36/41 inch poster.
Finally the RCM photo print of the purified human islets was pasted in the poster at the spot at the venue in Lyon.

Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla (open source app) with nice results. Only minor issues appeared with text spacing of the top row of the table , and the caption in the photo section. Some color is showing in the graphs , not evident on my grayscale equipment in the 90s. …. The poster image of 20240731 has been replaced by a remastered image , see Note Update 20240808 below.

Notes

1. Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2022/08/22/minneapolis-1989-poster-uw-solution-islet-isolation-20220822/

2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

6. Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media | URL https://michelvanderburg.com/2022/11/25/pancreatic-islet-transplantation-thesis-repository-leiden-university-20221125/

Update 20240808

Remastered poster ‘Lyon 1991 Percoll Poster | 20240731 • 20240808’

Following the first publication here last month (20240731) of the original 1991 MacDraw poster opened (converted) in LibreOffice Vanilla (1), I learned how to do some editing in the LibreOffice app to further restore poster layout details such as line spacing, and font style. An archive photo of another poster presentation in Pittsburgh in 1992 (that will be posted here soon) guided this restoration.

Current workflow :
1) opening the MacDraw II file in LibreOffice Vanilla
2) editing in in LibreOffice Vanilla , saving as LibreOffice Vanilla file (OpenDocument Drawing – extension odg).
3) opening the photo tiff image in the Mac’s Preview app, and copying the displayed image to the clipboard
4) pasting the clipboard image in the LibreOffice doc , and dragging to adjust size and location
5) exporting the file as PDF (image resolution 300 DPI ; PDF/A-1b ; PDF/UA).
6) Converting the PDF in the Preview app to TIFF 144 pixels/inch.
7) Adding caption , metadata etc with JPEG conversion in PhotoMill.

This 20240731 post now has this updated 20240808 – poster image.

Citation info : Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com

Berlingen • 20240712

Berlingen, Belgium. May 20, 2011.

After running through the woods all night, after his escape from Transport XX, the 11-year-old Simon Gronowski went to this house – a simple house, he trusted – at early dawn on Tuesday April 20th 1943. At the time, the number 14 in the Langstraat street [now renumbered 42] in Berlingen, Belgium.


Mrs Reynders answered the door when Simon rang the bell, and was the first to help Simon to get back to Brussels.

Photo (edited) by Michel van der Burg, taken close to midnight on May 20, 2011.

 Citation info : Berlingen | Miracles•Media | 20240712 | TakeNode deacfa40-7fc2-47e3-b1d6-af487a54bff7

Kuttekoven Stop Transport XX | Miracles Docs #5 | 20240708

Mrs Mia Valkeneers recounts her childhood memories, how she and her brother Marcel – both circa Simon Gronowski’s age – were living in Kuttekoven in a house at the railroad track at the time, some 50 meters from the site Simon Gronowski escaped from the 20th deportation train to Auschwitz, April 19, 1943.
That night they woke up, when the train had stopped, hearing the hissing of the locomotive, the Nazis shooting and shouting, and people crying during the escapes from the train, and…the next day, brother Marcel watched an open (flat) car passing by at the track with dead bodies…

Podcast at Miracles•Media – link
https://miracles.media/2024/07/08/kuttekoven-stop-transport-xx-miracles-docs-5-20240708/

Kuttekoven 2014 • Miracles Making • 20240707

Newspaper image Jan 28, 2014 | André Rouvrois | Het Belang van Limburg | URL https://www.hbvl.be/cnt/eid201382# | Miracles•Media | 20240707_01

Miracles Making… behind the scenes : Kuttekoven (Borgloon, Belgium), Jan 26, 2014. Filming Simon Gronowski’s talk in Clee Castle, and afterwards interviewing Mrs. Mia Valkeneers who recounted her childhood experience with the escapes from Transport XX in Kuttekoven.

Filming Simon Gronowski’s talk in Clee Castle, Kuttekoven, Jan 26, 2014 | Miracles•Media | 20240707_02 | TakeNode 2ecf8775-017b-4ea4-a019-a7b47aaf1a8a

Then, with a select company of some 10 residents of Kuttekoven, we went to the former train track, and while it started to rain — and one of the women bravely assisted me by holding my umbrella while I was recording on video – Simon spoke about his escape at that very spot at the track – the spot we had discovered two years earlier, Jan. 2012.

Still film Simon Gronowski’s talk in Clee Castle, Kuttekoven, Jan 26, 2014 | Miracles•Media | 20240707_03 | TakeNode b72f0ee1-0524-408a-9852-d06c2d4e090f

Later, that night while driving home (a ca 2 hour drive to Holland) – I was no longer thinking about perhaps paying Max De Vries a visit – the former resistance fighter, who had become 100 y old that month. But, by serendipity, when I crossed the town, Wellen, were Max lived, I briefly noticed , through the pouring rain, the road sign were Max lived, and in a reflex turned into that street, stopped somewhere – it was raining too hard for me to be able to look up Max’s house number in my papers – so I just rang a bell of one of the houses, and this turned out to be a neighbour, living 2 houses away from Max’s place. For 15 minutes (I really needed to continue that difficult ca 2 hours rainy night drive home) I was in the cosy kitchen talking with Max at the kitchn table and his wife Nicole, and congratulated him being 100 (no images made). Max passed away October 2014.

Citation info : Kuttekoven 2014 | Miracles Making | Miracles•Media | 20240707