Scan of print (10×15 cm; photo G.J. v.d. Poort) of oil- acrylic painting (60x50cm) by Hans Dudart.
Hans Dudart (1955-2003) worked as an artist in Holland, and as a valued co-worker in our Experimental Surgical Laboratory of the Leiden University Hospital (AZL, now LUMC) in Leiden, Holland. He died too soon from type 1 diabetes.
Citation info : No Title • Michel van der Burg • Miracles.Media • @1MEMO 20250112 • URL michelvanderburg.com/2025/01/12/
Slide film of May 11, 1989 developed by Audio Visual Center Leiden University.
Purified islets after using UW solution as the isolation medium, immuno-stained for insulin.
Citation info : Pure Islets • Michel van der Burg • Miracles.Media • @1MEMO 20250111 • URL michelvanderburg.com/2025/01/11/
Poster (1) presented at the 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991. The poster was originally created on a Macintosh computer Oct 6, 1991, using the MacDraw II application, with graphs from Cricket Graph. That 1991 poster was remastered today in LibreOffice.
We presented 2 posters at the 5th ESOT, this poster (1), and another one on the effects of denervation with pancreas transplantation (2).
Two years after the 4th ESOT (in Barcelona, Nov 1989) where we introduced the UW-Solution as an islet isolation solution before density gradient purification, we now had a talk at this 5th ESOT on the use of the UW-Solution as the islet isolation solution, both before purification, and also during the purification in an innovative density gradient (3) — the foundation for current clinical purification methods. The talk was recorded on tape (except for the intro), and will be posted here soon too.
1) Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.
2) Guicherit OR, Van der Burg MPM, Lemkes HHPJ, Frölich M, Gooszen HG. Pancreatic
denervation causes reduced insulin sensitivity. 5 th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) Oct 7–10 1991.
3) Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.
Citation info : Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109 • URL michelvanderburg.com/2025/01/09/
I started an archive at Archive.org November 11, 2010 with this slideshow (pdf) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).
The presented results of this innovative islet purification method — developed over nearly 10 years (from 1989) in our Leiden islet laboratory (LUMC) — were the outcome of work I had done spring 1998 with the human islet transplantation group of Camillo Ricordi at the Diabetes Research Institute in Miami , Florida, USA.
Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999
Slides oral presentation Michel van der Burg, Aidspit meeting 25 Jan 1999, in Igls, Austria
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification. 18th Workshop Aidspit, Igls (Austria) Jan. 24-26, 1999
Program :
18TH WORKSHOP OF THE AIDSPIT STUDY GROUP – JANUARY 24-26, 1999 – IGLS/AUSTRIA . MONDAY, JAN 25, 1999 – KONGRESSHAUS IGLS, MAIN LECTURE HALL
SESSION 2 – ISLET ISOLATION AND TRANSPLANTATION
CHAIRMEN:K. FEDERLIN, GIESSEN, GERMANY – G. POZZA, MILAN, ITALY 9.00 – 10.30 FREE COMMUNICATIONS
9.45- Efficacy of the novel iodixanol-UWS density gradient for human islet purification.
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C; Leiden, The Netherlands and Miami, USA
File : mvdb19990118_1534_AIDSPIT.pdf | Uploaded 20101111 and replaced by the same pdf with updated metadata of the document on 20101112 (see Notes section).
Citation info (Archive file) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL https://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet • TakeNode 54cc8b66-672e-4b13-913c-449038ed0521
The slideshow video embedded below, was first posted at 1-memo.com in 2018 as Human Islet Purification Miami 1998 • @1MEMO 20181102
The slideshow pdf file is available for download at Archive.org. I advise against the other download options presented by Archive.org , which do not correctly represent the slideshow.
The file was also uploaded to Scribd at the same time – URL https://www.scribd.com/document/42197150/Efficacy-of-the-novel-iodixanol-UWS-density-gradient-for-human-islet-purification-michelvanderburg-19990125
Abstract
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Abstract). Acta Diabetol 1998; 35: 247. View or download abstract below .
Updates
20251101 – Vimeo video replaced by YouTube copy
20251101 – Added PDF copy abstract – file 1MEMO_20251102_1.pdf
Citation info (this post) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL michelvanderburg.com/2024/12/02/
Sinclair’s ZX81 computer (1KB on-board memory , with a 64K memory expansion module inserted at the back) – my first PC – that I started using from 1983 for automation of work in our experimental pancreas and islet transplantation research project – for creating (‘coding’) and using the entire suit of apps, needed for the raw data processing and the (statistical and pharmacokinetic) analysis.
The hardware set-up included a TV (B/W) as monitor, (mono audio) tape recorder for data storage (apps), and a small thermal printer.
Citation info : ZX81 • Michel van der Burg • Miracles•Media • 20241018 • TakeNode 9c595df8-8091-497c-9c9c-ff85fe4de397 • URL michelvanderburg•com
While searching my archive today, I encountered the Press release Sep 27, 2001 of the dutch National Health Care Institute (CVZ), advicing the Ministry of Health, Welfare and Sport (VWS) to grant permission for Transplantation of Islets of Langerhans in the Leiden University Medical Center (LUMC).
That press release is included now (in note 6) in the recent post on the Leiden Islet Laboratory History at the site – https://michelvanderburg.com/2024/08/03/leiden-islet-laboratory-history-20240803/
Citation info : Press Release • Michel van der Burg • Miracles•Media • 20241010 • URL michelvanderburg.com/2024/10/10/
Funny. LibreOffice Vanilla Issue.
Greek characters appeared in this poster originally designed with english text and produced on a Macintosh computer and Imagewriter dotmatrix printer, September 3, 1990, using the MacDraw II application, with graphs from Cricket Graph.
That 1990 Mac file was remastered today in LibreOffice Vanilla (other minor issues in graphs not corrected). Generally LibreOffice does a great job with these ancient files. I recently posted several of such posters at site michelvanderburg.com.
However, some of the posters retrieved from my archive show up with Greek characters when opened in LibreOffice, like this poster produced in English (with roman characters).
Those problematic posters have in common that the Apple Chicago font was used – the interface font in Apple’s early versions of Mac OS from 1984 to 1997. Clearly LibreOffice cannot handle this Chicago font correctly. I have not found a solution for this.
Text in other posters, produced with Helvetica, had only minor issues in LibreOffice, Helvetica italics text appeared as regular Times.
The original poster and abstract (below) were presented at the 8th International Symposium on Gastrointestinal Hormones, in Timmendorfer Strand Baltic Sea (FRG) September 4–8, 1990.
Contribution of gut factors to canine isolated islet function
M.P.M. van der Burg, O.R. Guicherit, J.B.M.J. Jansen, C.B.H.W. Lamers, J.P. Scherft, M. Frölich, H.G. Gooszen.
Departments of Surgery, Gastroenterology. Endocrinology and Cel Biology. University Hospital, Leiden, The Netherlands.
Techniques for isolating islets of Langerhans from the large mammalian pancreas have improved to the point where islet transplantation as a therapeutical approach to human diabetes has become more realistic. The quality of metabolic control is however largely unknown. We studied canine isolated-islet function both in vivo after autotransplantation, as well as in vitro by perifusion.
Five normal dogs underwent total pancreatectomy. Islets were isolated from the excised pancreas by collagenase digestion, dispersion and purification with filtration and density gradients. Isolated islets were autotransplanted into the spleen of the dog by retrograde venous infusion. Graft function was assessed up to 3 mo by determining the glucose and insulin response to an intravenous glucose injection (IVGTT), i.v. arginine injection during 35 mM glucose clamp (AT), and a meal. In addition (n = 4) the in vitro insulin response of overnight cultured canine islets was studied by perifusion with cholecystokinin (CCK-33) and glucose-dependent insulinotropic polypeptide (GIP).
The islet dose at transplantation ranged from 3500 – 13000 islets/kg b.w. One animal became overtly hyperglycemic (fasting glucose 18 mM) within 7 days after receiving 3500 islets/kg b.w. The other grafts (> 6000 islets/kg b.w.) were successful (fasting glucose <7mM) but demonstrated, compared to pre-operative values, a 50% reduced glucose clearance and insulin response at IVGTT, and a 90% reduced insulin secreting capacity at AT. Postprandially hyperglycemia (~ 10 mM) and in contrast to i.v. glucose and arginine, a normal insulin response was observed. The in vitro insulin response at 7.5 mM glucose to equimolar (0.1-1- 10 nM) concentrations of either CCK, GIP or both, demonstrated a sustained dose-response to GIP (up to 5x basal values) and a significant albeit small and transient effect of CCK as of 1 nM. No synergistic effect was observed.
In dogs ‘one-to-one’ transplantation was successful in 4/5 recipients of isolated islets. The difference in the effect of islet transplantation on the insulin response to intravenous glucose or – arginine and a meal, may be related to the postprandial, hyperglycemia enhanced, activation of the entero-insular axis, especially GIP.
Van der Burg MPM, Guicherit OR, Jansen JBMJ, Lamers CBHW, Scherft JP, Frölich M, Gooszen HG. Contribution of gut factors to canine isolated islet function (Abstract). Digestion 1990; 46 (Suppl 1): 14–15. https://doi.org/10.1159/000200356
PDF abstract shown below – file 20241009_1_Digestion.pdf
Citation info : Greek Poster • Michel van der Burg • Miracles•Media • 20241009 • URL michelvanderburg.com/2024/10/09 | TakeNode 10afaddd-08b7-4dcd-ad7a-43228420ad59
