Pittsburgh (USA) May 31–June 3, 1992. Founding meeting, and 1st Int. Congress of the Cell Transplant Society.
Michel van der Burg, Co-chair ‘Methods’ Poster Discussion CTS , Pittsburgh June 3, 1992 • 20240812_2 • michelvanderburg•com • TakeNode 509ce973-d218-4f9c-84ef-ffa7c4fdb0d1
Poster creation
The poster was designed and produced May 1992 on a Macintosh computer using MacDraw II (with bar graphs from Cricket Graph) and printed landscape across a chain of several sheets of paper using the Imagewriter dotmatrix printer.
For this 2024 remastered poster below, the 1992 MacDraw II file was converted and edited using LibreOffice Vanilla, and next Pixelmator Pro was used for additional ‘repair’ (conversion issues with the many layers used for ‘tubes’ creation) and the insertion of the photo scans.
Van der Burg MPM, Guicherit OR, Scherft JP, Bruijn JA, Frölich M, Gooszen HG. Islet preservation during isolation: a new concept in cell transplantation. First International Congress of the Cell Transplant Society, Pittsburgh (USA) May 31 – June 3, 1992.
Van der Burg MPM, Guicherit OR, Frölich M, Bruijn JA, Gooszen HG. Islet preservation during isolation: a new concept in cell transplantation. Transplant Proc. 1992 Dec;24(6):2840-1. PMID: 1465965. PDF available below PMID_1465965 🔗
Update dd 20250115 – proceedings paper PMID: 1465965 added
Citation info : Pittsburgh 1992 Cell Transplant Society • 20240812 • Michel van der Burg • michelvanderburg•com
Shortly after starting our Islet Lab end 1986, I hand-picked and photographed this special big heart-shaped isolated islet of Langerhans with Uncle Scrooge McDuck’s ‘lucky #1 dime’ in mind.
(dutch : Dagobert Duck’s geluksdubbeltje). This ‘First Islet’ poster was pinned to the wall in my study in the (small) Islet Lab ‘wing’ in the Cell Biology building of the Leiden University Hospital.
It’s an Islet of Langerhans isolated from a rat pancreas. For pilot work, the first months while developing our islet project plan, islets were isolated from rat pancreases.
I had started developing our basic lab methods… like eg. measuring the insulin secretion capacity and insulin content of isolated islets. A custom-made glass pipet was used to hand-pick and transfer the islets to little polypropyleen ‘Eppendorf’ tubes for insulin secretion tests with glucose stimulation.
Citation info : Lucky #1 Islet • 20240811 • Michel van der Burg • michelvanderburg•com | TakeNode 5234a6e0-4565-4167-bb46-0f922c0b1d2a
Friday, September 2, 1983 talk by Hein Gooszen, on experimental pancreas transplantation (1), at the International Symposium on Organ Transplantation in Diabetics (2) in the Hall of Knights (dutch : Ridderzaal), Binnenhof (English: Inner Court), The Hague, Netherlands.
Notes
1. Hein Gooszen, Reinout van Schilfgaarde, Marijke Frölich, Michel van der Burg (Leiden, The Netherlands). Long-term endocrine function of ductobliterated in situ and autografted left canine pancreatic segments. International Symposium on Organ Transplantation in Diabetics, The Hague, The Netherlands, September 1–2, 1983.
2. Reinout van Schilfgaarde (Leiden, The Netherlands) chaired the organizing and scientific committee.
Citation info : Organ Transplantation 1983 Symposium • 20240810 • Michel van der Burg • michelvanderburg•com • TakeNode (20240810_1) 37e1dc78-b59f-4305-8441-55f8d02cce6b • TakeNode (20240810_2) 57e8de9e-639b-422b-a567-bc1399dd1b4e
September 1989 at a symposium of two international societies in Minneapolis, USA (1) , I presented this first poster on the ‘ invention ‘ of using the UW solution ( the University of Wisconsin organ preservation solution) during pancreatic islet isolation, with the exciting discovery of near-complete purification of islets of Langerhans in the laboratory for transplantation in diabetic patients.
Here the remastered 20240804 edition of that Minneapolis 1989 poster presentation.
A previous edition of the remastered poster was published here two years ago (2) but that edition had a different photo print (higher magnification, and without the bar indicator) of the purified islet preparation inserted, not the original image. This has been corrected now. In addition the original line spacing is now restored.
Notes
1) Van der Burg MPM, Gooszen HG, Ploeg RJ, Scherft JP, Field MJ, Guicherit OR, Terpstra JL, Van de Woude FT, Frölich M, Bruijn JA. Comparison of islet isolation techniques in dogs: Over 90% purified islets using UW solution. 4th International Symposium on Organ Procurement and Preservation and 2nd International Congress on Pancreatic and Islet Transplantation, Minneapolis (USA) September 17–22, 1989.
2) Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2022/08/22
Updates
20241211 : Minor text updates
Citation info : Minneapolis 1989 Poster | 20240804 | Michel van der Burg | michelvanderburg•com | TakeNode 91d70715-3fb3-4380-906a-0daf1b5ddd6a
Leiden Islet Laboratory History • 20240803 | Michel van der Burg | michelvanderburg•com | TakeNode fad1be86-34f2-453f-b477-2abc62e467be
Summer 1985… start of our Leiden Islet Transplant Laboratory by Paul van Suylichem (right) and Michel van der Burg (middle), both from the Surgery department — wondering how to start here our islet lab in this space – a former weighing chamber (left : stable weighing table) made available by the Cell Biology department of the Leiden University, headed by Prof J.P. Scherft. Where to put our equipment… the laminar flow cabinet, large centrifuge, fridge, freezer, shaking waterbath, microscopes, and a lot of disposables coming ?
I had started working in 1981 for the Pancreas project. Pancreas transplantation research with the surgeon Hein Gooszen , working on his PhD (1), and surgeon Reinout van Schilfgaarde – who headed the project – and in 1985 also started this islet project.
Summer 1986, when Reinout van Schilfgaarde together with Paul van Suylichem moved to the Groningen University Hospital (2), Hein Gooszen and I decided to make a fresh start with the now operational Leiden Islet Lab…
Fifteen years of research later we were ready to roll with the isolation of human islets of Langerhans, in our new GMP facility in the Leiden Univerity Medical Center (LUMC). September 2001, the National Health Care Institute positively assessed our permit application for Transplantation of Islets of Langerhans in the LUMC (3,6), followed by approval May 2003 of the islet bank by the Health and Youth Care Inspectorate of the Ministry of Health, Welfare and Sport (VWS).
Notes
Post last update : 20241010-1611
Gooszen HG. Canine segmental pancreatic autotransplantation. Analysis of the effects of ductobliteration. Thesis, Leiden, 1984
Advies vergunningsaanvraag transplantatie Eilandjes van Langerhans | Op 27 september 2001 uitgebracht aan de minister van Volksgezondheid, Welzijn en Sport. | Rapport 01/71 | C.R. Smand, J.P. Verduijn / College voor Zorgverzekeringen. URL https://data.bibliotheken.nl/id/nbt/p229940978
Update 20240807. Added some history, and made minor changes.
Update 20240828 minor correction date (Fresh start lab ‘At the end of 1986’ changed to Summer 1986.)
Update 20241010, added : Press release Sep 27, 2001 by National Health Care Institute (CVZ). Transplantatie Eilandjes van Langerhans naar LUMC 27 september 2001. CVZ adviseert over vergunning. John van der Pas, persvoorlichter CVZ. Source : Razende Robot Reporter | Nieuwsbank Interactief Nederlands persbureau . Retrieved 20090313 from Nieuwsbank.nl (link expired, domain has new owner). 20241010 PDF (Ads , and author phone number redacted by M.P.M. van der Burg | Miracles•Media) file 20241010_CVZ • URL michelvanderburg.com/2024/10/10
Citation info : Leiden Islet Laboratory History • 20240803 | Michel van der Burg | michelvanderburg•com | TakeNode fad1be86-34f2-453f-b477-2abc62e467be
Following first introduction of the use UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options.
One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS (7) . Note that Pentastarch is a component of UWS.
The Percoll-UWS poster was posted recently (8)
Here the remastered poster presentation on Pentastarch-UWS :
Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 3 strips) were then glued on site along the perforation, creating the 36/24 inch poster.
Finally the photo print of the purified islets was pasted in the poster at the spot at the venue in Lyon. In Lyon a photo of a dithizone stained Pentastarch purified islet sample was shown. To save time the archive was not searched for that image, and another image of Pentastarch purified islets was inserted in this remastered edition, a reflection contrast micrograph of an ultrathin section of Pentastarch purified islets immuno-stained with gold on insulin in the islet cells.
Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla and now learned to use the app to further correct most conversion issues. Pixelmator Pro was used for additional ‘repair’ and insertion of the photo scan.
2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
7. Pentastarch is the synthetic colloid component of UWS (ViaSpan / Belzer solution), a purified fraction (250 kD) of hydroxyethyl starch. Pentastarch powder was used here to increase the Pentastarch concentration and density of the UWS.
8. Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com
Citation info : PentaStarch UWS Pancreatic Islet Purification • 20240802 | Michel van der Burg | michelvanderburg•com | TakeNode d11f1cef-a343-4466-8814-3e52a0261cae
Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e
Following first introduction of the use of UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options. One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS . Note that Pentastarch is a component of UWS.
Here one of these poster presentations, on Percoll-UWS : Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application, inserted with a bar graph prepared in the Cricket Graph app. The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 5 strips) were then glued on site along the perforation, creating a 36/41 inch poster. Finally the RCM photo print of the purified human islets was pasted in the poster at the spot at the venue in Lyon.
Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla (open source app) with nice results. Only minor issues appeared with text spacing of the top row of the table , and the caption in the photo section. Some color is showing in the graphs , not evident on my grayscale equipment in the 90s. …. The poster image of 20240731 has been replaced by a remastered image , see Note Update 20240808 below.
2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
Following the first publication here last month (20240731) of the original 1991 MacDraw poster opened (converted) in LibreOffice Vanilla (1), I learned how to do some editing in the LibreOffice app to further restore poster layout details such as line spacing, and font style. An archive photo of another poster presentation in Pittsburgh in 1992 (that will be posted here soon) guided this restoration.
Current workflow : 1) opening the MacDraw II file in LibreOffice Vanilla 2) editing in in LibreOffice Vanilla , saving as LibreOffice Vanilla file (OpenDocument Drawing – extension odg). 3) opening the photo tiff image in the Mac’s Preview app, and copying the displayed image to the clipboard 4) pasting the clipboard image in the LibreOffice doc , and dragging to adjust size and location 5) exporting the file as PDF (image resolution 300 DPI ; PDF/A-1b ; PDF/UA). 6) Converting the PDF in the Preview app to TIFF 144 pixels/inch. 7) Adding caption , metadata etc with JPEG conversion in PhotoMill.
This 20240731 post now has this updated 20240808 – poster image.
Citation info : Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com
