Transplants of UW-Isolated Islets • Transplantation Society 1990 Paper • @1MEMO 20250118

Congress of The Transplantation Society, Lobby of the Fairmont Hotel, San Francisco, California, USA, August 1990 • Michel van der Burg • Miracles.Media • @1MEMO 20250118_1 • TakeNode 4af71bca-edaa-4adb-9dce-556534844f5b

Following papers presented in 1989 demonstrating that over 90% pure islets are isolated from pancreases when using the UW organ preservation solution (1), the outcome of transplantation of highly purified UW-isolated islets was reported in a proceedings paper presented August 1990 at the XIII International Congress of The Transplantation Society in San Francisco, USA (2,3).
The Transplantation Proceedings paper, published Feb 1991 is now available online below (3).

Notes

1) Pancreatic Islet Isolation With UW Solution • A New Concept • Michel van der Burg • Miracles.Media • @1MEMO 20241224 • URL michelvanderburg.com/2024/12/24/

2) Van der Burg MPM, Guicherit OR, Ploeg RJ, Frölich M, Bruijn JA, Scherft JP, Gooszen HG.
Metabolic control after autotransplantation of highly purified canine pancreatic islets isolated in UW-solution. XIII International Congress of The Transplantation Society, San Francisco (USA) August 19–24, 1990.

3) van der Burg MPM, Guicherit OR, Ploeg RJ, Frölich M, Bruijn JA, Scherft JP, Gooszen HG. Metabolic control after autotransplantation of highly purified canine pancreatic islets isolated in UW solution. Transplant Proc. 1991 Feb;23(1 Pt 1):785-6. PMID: 1990691. PDF available below

PMID_1990691🔗Download

Citation info : Transplants of UW-Isolated Islets • Transplantation Society 1990 Paper • Michel van der Burg • Miracles.Media • @1MEMO 20250118 • URL michelvanderburg.com/2025/01/18/

Leiden Islet Isolation Chamber • @1MEMO 20250116

Leiden Islet Isolation Chamber • Michel van der Burg • Miracles.Media • @1MEMO 20250116 • TakeNode 398303fe-928a-4c53-839e-9dde0e7d8ec6

Following a working visit February ’92 studying pig islet isolation at the ‘Fondation Transplantation’ in Strasbourg -Hautepierre, France (1) , I designed this islet isolation chamber March 1992 for use in our Leiden Islet Laboratory , together with Gerard Verschagen , head of the precision mechanics workshop of the Laboratory of Physiology of the University Hospital Leiden (AZL , now LUMC), who manufactured this and other of our islet isolation devices.

The chamber was designed with support for use together with the Retsch Vibro Sieving Machine , purchased earlier for our Leiden Islet Laboratory, a vibrating sieving machine I had used in the 70s for isolation cell nuclei (2). The design was based on the Ricordi chamber published 4 years earlier (3).

The drawing was originally designed and produced on a Macintosh computer March 1992, using the MacDraw II application. That Mac file was remastered today in LibreOffice Vanilla, by straightening most of the lines that are slanted after opening in LibreOffice and correcting the font changes. So , not accurate now probably … just for illustration the history of progress in our Leiden Islet Laboratory, and love for art 😉

Notes

1) Xeno Islets • Michel van der Burg • Miracles.Media • @1MEMO 20250113 • URL michelvanderburg.com/2025/01/13/

2) Mennes AM, Bouman H, Van der Burg MPM, Libbenga KR. RNA synthesis in isolated tobacco callus nuclei, and the influence of phytohormones. Plant Sci Lett 1978; 13: 329– 339, ISSN 0304-4211, https://doi.org/10.1016/0304-4211(78)90210-9.

3) Piemonti L, Pileggi A. 25 Years Of The Ricordi Automated Method For Islet Isolation. CellR4 Repair Replace Regen Reprogram. 2013;1(1):e128. PMID: 30505878; PMCID: PMC6267808. URL https://www.cellr4.org/article/128

Citation info : Leiden Islet Isolation Chamber • Michel van der Burg • Miracles.Media • @1MEMO 20250116 • URL michelvanderburg.com/2025/01/16/

Xeno Islets • @1MEMO 20250113

Pig Islet Isolation, Fondation Transplantation, Strasbourg 1992 • Michel van der Burg • Miracles.Media • @1MEMO 20250113_1 • TakeNode b1f66781-cf63-4e64-a7b3-24cbdf5c107f

For the xenotransplantation research we started in 1991 at our Leiden Surgery Department, I studied pig islet isolation during a working visit February 1992, at the ‘Fondation Transplantation’ in Strasbourg-Hautepierre, France — here watching our student Vincent Nieuwenhuijs (left) at work in the international team – with Agnaldo Soares I guess – using the Ricordi method for islet isolation — and presented the recent Leiden islet work (1).

Notes

1) Van der Burg MPM, Gooszen HG. Islet preservation using UW, and islet function after
autotransplantation in dogs. Fondation Transplantation, CHU HautePierre Strasbourg
(France) February 18, 1992.

Update 20250124 – Paper reference

Citation info : Xeno Islets • Michel van der Burg • Miracles.Media • @1MEMO 20250113 • URL michelvanderburg.com/2025/01/13/

No Title • @1MEMO 20250112

No Title • Oil- Acrylic painting by Hans Dudart • Miracles.Media • @1MEMO 20250112_1 • TakeNode cbd20789-ebf5-4ede-925b-9b861c6719a4

Scan of print (10×15 cm; photo G.J. v.d. Poort) of oil- acrylic painting (60x50cm) by Hans Dudart.

Hans Dudart (1955-2003) worked as an artist in Holland, and as a valued co-worker in our Experimental Surgical Laboratory of the Leiden University Hospital (AZL, now LUMC) in Leiden, Holland. He died too soon from type 1 diabetes.

Citation info : No Title • Michel van der Burg • Miracles.Media • @1MEMO 20250112 • URL michelvanderburg.com/2025/01/12/

Pure Islets • @1MEMO 20250111

Pure Islets • Michel van der Burg • Miracles.Media • @1MEMO 20250111_1 • TakeNode a14f79f7-b04a-41ff-ab27-1de482a9fe4c

Slide film of May 11, 1989 developed by Audio Visual Center Leiden University.

Purified islets after using UW solution as the isolation medium, immuno-stained for insulin.

Citation info : Pure Islets • Michel van der Burg • Miracles.Media • @1MEMO 20250111 • URL michelvanderburg.com/2025/01/11/

Islet Transplants Performance 5th ESOT 1991 • @1MEMO 20250109

Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109_1 • TakeNode 75edbb4e-4360-43b8-8783-363ffd34c0ef

Poster (1) presented at the 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991. The poster was originally created on a Macintosh computer Oct 6, 1991, using the MacDraw II application, with graphs from Cricket Graph. That 1991 poster was remastered today in LibreOffice.

We presented 2 posters at the 5th ESOT, this poster (1), and another one on the effects of denervation with pancreas transplantation (2).

Two years after the 4th ESOT (in Barcelona, Nov 1989) where we introduced the UW-Solution as an islet isolation solution before density gradient purification, we now had a talk at this 5th ESOT on the use of the UW-Solution as the islet isolation solution, both before purification, and also during the purification in an innovative density gradient (3) — the foundation for current clinical purification methods. The talk was recorded on tape (except for the intro), and will be posted here soon too.

Notes

1) Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.

2) Guicherit OR, Van der Burg MPM, Lemkes HHPJ, Frölich M, Gooszen HG. Pancreatic
denervation causes reduced insulin sensitivity. 5 th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) Oct 7–10 1991.

3) Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.

Citation info : Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109 • URL michelvanderburg.com/2025/01/09/

DRI Dream Team • @1MEMO 20250105

DRI Dream Team • Miami 1998 • Michel van der Burg • Miracles.Media • 20250105_1 • TakeNode e6258505-55a8-4365-bb70-7a1dcd7b5426

Team up with researchers at the Diabetes Research Institute in Miami , working on human islet transplantation (1).

“For National Geographic…” announced Camillo Ricordi for fun, that Monday , April 27th in 1998 at the Diabetes Research Institute, when the DRI islet isolation team posed in GMP outfit for this picture (2) — with Elina Linetsky on the left , and me standing second from right — taken after finishing our human islet isolation work, including islet purification in the iodixanol gradient in UW-Solution (3).

Notes

1) Working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA)

2) Blown up image (from small GIF online in the early DRInet days), taken through a reflecting window.

3) Human Islet Purification Miami 1998 • Michel van der Burg • Miracles.Media • 20181102 • URL michelvanderburg.com/2018/11/02/

Citation info : DRI Dream Team • Michel van der Burg • Miracles.Media • @1MEMO 20250105 • URL michelvanderburg.com/2025/01/05/